{"title":"利用液相色谱-高分辨质谱法和多元分析估算指甲花产品的原料植物。","authors":"Naohiro Oshima, Maiko Tahara, Tsuyoshi Kawakami, Akiko Yagami, Takumi Akiyama, Nahoko Uchiyama, Yoshiaki Ikarashi","doi":"10.1248/cpb.c24-00278","DOIUrl":null,"url":null,"abstract":"<p><p>Henna is a plant-based dye obtained from the powdered leaf of the pigmented plant Lawsonia inermis, and has often been used for grey hair dyeing, treatment, and body painting. As a henna product, the leaves of Indigofera tinctoria and Cassia auriculata can be blended to produce different colour variations. Although allergy from henna products attributed to p-phenylenediamine, which is added to enhance the dye, is reported occasionally, raw material plants of henna products could also contribute to the allergy. In this study, we reported that raw material plants of commercial henna products distributed in Japan can be estimated by LC-high resolution MS (LC-HRMS) and multivariate analysis. Principal Component Analysis (PCA) score plot clearly separated 17 samples into three groups [I; henna, II; blended henna primarily comprising Indigofera tinctoria, III; Cassia auriculata]. This grouping was consistent with the ingredient lists of products except that one sample listed as henna was classified as Group III, indicating that its ingredient label may differ from the actual formulation. The ingredients characteristic to Groups I, II, and III by PCA were lawsone (1), indirubin (2), and rutin (3), respectively, which were reported to be contained in each plant as ingredients. Therefore, henna products can be considered to have been manufactured from these plants. 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The ingredients characteristic to Groups I, II, and III by PCA were lawsone (1), indirubin (2), and rutin (3), respectively, which were reported to be contained in each plant as ingredients. Therefore, henna products can be considered to have been manufactured from these plants. 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引用次数: 0
摘要
指甲花是一种植物染料,取自色素植物 Lawsonia inermis 的叶粉,常用于染白发、治疗和身体彩绘。作为指甲花产品,Indigofera tinctoria 和 Cassia auriculata 的叶子可以混合产生不同的颜色变化。虽然偶尔有报道称指甲花产品过敏是由于添加了对苯二胺来增强染料的效果,但指甲花产品的原料植物也可能导致过敏。在这项研究中,我们报告了通过液相色谱-高分辨质谱(LC-HRMS)和多元分析可以估算出在日本销售的商业指甲花产品的原料植物。主成分分析(PCA)得分图清楚地将 17 种样品分为三组[I;指甲花;II;主要由靛蓝组成的混合指甲花;III;决明子]。这种分组与产品的成分表一致,只有一个列为指甲花的样品被归为第 III 组,表明其成分标签可能与实际配方不同。根据 PCA,第 I 组、第 II 组和第 III 组的特征成分分别是律酮(1)、靛红素(2)和芦丁(3),据报告每种植物都含有这些成分。因此,可以认为指甲花产品是由这些植物制成的。本研究首次通过 LC-HRMS 和多元分析估算了用于商业植物染料的原料植物。
Estimation for Raw Material Plants of a Henna Product Using LC-High Resolution MS and Multivariate Analysis.
Henna is a plant-based dye obtained from the powdered leaf of the pigmented plant Lawsonia inermis, and has often been used for grey hair dyeing, treatment, and body painting. As a henna product, the leaves of Indigofera tinctoria and Cassia auriculata can be blended to produce different colour variations. Although allergy from henna products attributed to p-phenylenediamine, which is added to enhance the dye, is reported occasionally, raw material plants of henna products could also contribute to the allergy. In this study, we reported that raw material plants of commercial henna products distributed in Japan can be estimated by LC-high resolution MS (LC-HRMS) and multivariate analysis. Principal Component Analysis (PCA) score plot clearly separated 17 samples into three groups [I; henna, II; blended henna primarily comprising Indigofera tinctoria, III; Cassia auriculata]. This grouping was consistent with the ingredient lists of products except that one sample listed as henna was classified as Group III, indicating that its ingredient label may differ from the actual formulation. The ingredients characteristic to Groups I, II, and III by PCA were lawsone (1), indirubin (2), and rutin (3), respectively, which were reported to be contained in each plant as ingredients. Therefore, henna products can be considered to have been manufactured from these plants. This study is the first to estimate raw material plants used in commercial plant-based dye by LC-HRMS and multivariate analysis.
期刊介绍:
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