利用磁化功能化柔性微磁体阵列研究聚苯乙烯微珠在集成微流体系统中的聚焦和分离问题

IF 2.3 4区 工程技术 Q2 INSTRUMENTS & INSTRUMENTATION
Shuang Chen, Jiajia Sun, Zongqian Shi, Xiaofeng Liu, Yuxin Ma, Ruohan Li, Shumin Xin, Nan Wang, Xiaoling Li, Kai Wu
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引用次数: 0

摘要

利用微流体技术聚焦和分离细胞是单细胞分析和疾病诊断等许多应用中的重要步骤。在微流体技术中,无源磁泳作为一种无标记的方式,可以通过磁场操纵样品。目前,磁场大多由体积较大的永久磁铁和电磁铁产生。然而,使用永久磁铁或电磁铁组装磁阵列以优化磁场分布却很困难。为了产生柔性磁场,本文提出了一种由钕铁硼粉末和聚二甲基硅氧烷制成的微型磁体,并根据磁化方向的排列将这些磁化的微型磁体排列成不同的阵列。同时,设计了一种包含磁化微磁体阵列的微流控芯片,用于聚焦和分离不同直径的聚苯乙烯微珠。通过数值和实验研究了微珠在所设计的微流控系统中的聚焦和分离行为。此外,还讨论了流速和磁性微磁体阵列排列对微珠聚焦和分离的影响。最后,设计了一种多级微流控芯片,可在 240 μL/min 的流速下成功地从混合物中分离出直径为 5 μm、10 μm 和 15 μm 的微珠,且分离纯度很高。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Investigation on the focusing and separation of polystyrene microbeads in an integrated microfluidic system using magnetized functionalized flexible micro-magnet arrays

Investigation on the focusing and separation of polystyrene microbeads in an integrated microfluidic system using magnetized functionalized flexible micro-magnet arrays

Investigation on the focusing and separation of polystyrene microbeads in an integrated microfluidic system using magnetized functionalized flexible micro-magnet arrays

Focusing and separation of cells by microfluidic techniques are significant steps in many applications, such as single-cell analysis and disease diagnosis. Among the microfluidic techniques, passive magnetophoresis, as a label-free manner, can manipulate samples by means of magnetic field. Nowadays, most magnetic fields are generated by permanent magnets and electromagnets with large size. However, it is difficult to assemble a magnetic array using permanent magnets or electromagnets to optimize the field distribution. To produce a flexible magnetic field, a micro-magnet made by NdFeB powder and polydimethyl siloxane is proposed in this paper, and those magnetized micro-magnets are arranged into different arrays according to the arrangements of their magnetization directions. Meanwhile, a microfluidic chip containing magnetized micro-magnet arrays is designed for focusing and separating polystyrene microbeads with different diameters. The focusing and separation behaviors of microbeads in the designed microfluidic system are numerical and experimental investigated. In addition, the effects of flow rate and the arrangement of the magnetic micro-magnet array on microbead focusing and separation are discussed. Finally, a multistage microfluidic chip is designed to successfully isolate 5 μm-diameter, 10 μm-diameter, and 15 μm-diameter microbeads from their mixture at a flow rate of 240 μL/min with high purity.

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来源期刊
Microfluidics and Nanofluidics
Microfluidics and Nanofluidics 工程技术-纳米科技
CiteScore
4.80
自引率
3.60%
发文量
97
审稿时长
2 months
期刊介绍: Microfluidics and Nanofluidics is an international peer-reviewed journal that aims to publish papers in all aspects of microfluidics, nanofluidics and lab-on-a-chip science and technology. The objectives of the journal are to (1) provide an overview of the current state of the research and development in microfluidics, nanofluidics and lab-on-a-chip devices, (2) improve the fundamental understanding of microfluidic and nanofluidic phenomena, and (3) discuss applications of microfluidics, nanofluidics and lab-on-a-chip devices. Topics covered in this journal include: 1.000 Fundamental principles of micro- and nanoscale phenomena like, flow, mass transport and reactions 3.000 Theoretical models and numerical simulation with experimental and/or analytical proof 4.000 Novel measurement & characterization technologies 5.000 Devices (actuators and sensors) 6.000 New unit-operations for dedicated microfluidic platforms 7.000 Lab-on-a-Chip applications 8.000 Microfabrication technologies and materials Please note, Microfluidics and Nanofluidics does not publish manuscripts studying pure microscale heat transfer since there are many journals that cover this field of research (Journal of Heat Transfer, Journal of Heat and Mass Transfer, Journal of Heat and Fluid Flow, etc.).
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