对Alhagi sparsifolia幼苗在氮添加条件下适应干旱胁迫的深入研究:糖代谢、激素和抗氧化潜力的调控

IF 3.4 3区 农林科学 Q2 ENVIRONMENTAL SCIENCES
Abd Ullah, Akash Tariq, Fanjiang Zeng, Muhammad Ahsan Asghar, Jordi Sardans, Josep Peñuelas
{"title":"对Alhagi sparsifolia幼苗在氮添加条件下适应干旱胁迫的深入研究:糖代谢、激素和抗氧化潜力的调控","authors":"Abd Ullah, Akash Tariq, Fanjiang Zeng, Muhammad Ahsan Asghar, Jordi Sardans, Josep Peñuelas","doi":"10.1007/s42729-024-01814-w","DOIUrl":null,"url":null,"abstract":"<p>Mature xerophytes access groundwater and minimize the risk of water and nutrient deficits in arid environments. However, how their young seedlings respond to the availability of water and nutrients before they reach groundwater is largely unknown. We investigated the effects of different drought regimes (controlled, medium-drought (MD), and severe-drought (SD)] and nitrogen (N; with or without) addition on biomass and physio-biochemical responses in <i>Alhagi sparsifolia</i> seedlings. Both drought stresses significantly increased superoxide dismutase (O<sub>2</sub><sup>•−</sup>), hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), malondialdehyde, and oxidized-glutathione in leaves and roots, thereby impairing growth and metabolism. Furthermore, there is a significant accumulation of fructose and glucose, but lower sucrose and starch, possibly due to higher sucrose synthase, α-amylase, β-amylase and hexokinase but lower sucrose phosphate synthase and fructokinase. Drought-stressed seedlings also displayed higher abscisic and, jasmonic acids, strigolactones, glucose-6-phosphate dehydrogenase (G-6-PDH), phosphoenolpyruvate carboxylase, O<sub>2</sub><sup>•−</sup>-H<sub>2</sub>O<sub>2</sub>-scavenging enzymes, but lower gibberellin, cytokinin, and indole-acetic acid. However, N-addition quantifies the productivity of drought-stressed seedlings by improving the leaf relative water content (LRWC), biomass, chlorophyll-a, sucrose-synthesizing enzymes (SPP and SPS), and hormones. It also increased the G-6-PDH in stressed seedlings to satisfy the need for NADPH and reduced the sucrose and starch degrading enzymes, leading to higher starch and sucrose levels. Upregulation of O<sub>2</sub><sup>•−</sup>-H<sub>2</sub>O<sub>2</sub> -scavenging enzymes under N-supply reduced lipid peroxidation and improved the ascorbate–glutathione redox states. N addition might be an effective strategy to improve drought resistance in <i>A. sparsifolia</i> seedlings to manage and conserve its vegetation in hyper-arid conditions in the face of future climate change.</p>","PeriodicalId":17042,"journal":{"name":"Journal of Soil Science and Plant Nutrition","volume":null,"pages":null},"PeriodicalIF":3.4000,"publicationDate":"2024-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Insights into Alhagi sparsifolia Seedlings Adaptations to Drought Stress under Nitrogen Addition: Regulation of Sugar Metabolism, Hormones, and Anti-Oxidant Potential\",\"authors\":\"Abd Ullah, Akash Tariq, Fanjiang Zeng, Muhammad Ahsan Asghar, Jordi Sardans, Josep Peñuelas\",\"doi\":\"10.1007/s42729-024-01814-w\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Mature xerophytes access groundwater and minimize the risk of water and nutrient deficits in arid environments. However, how their young seedlings respond to the availability of water and nutrients before they reach groundwater is largely unknown. We investigated the effects of different drought regimes (controlled, medium-drought (MD), and severe-drought (SD)] and nitrogen (N; with or without) addition on biomass and physio-biochemical responses in <i>Alhagi sparsifolia</i> seedlings. Both drought stresses significantly increased superoxide dismutase (O<sub>2</sub><sup>•−</sup>), hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), malondialdehyde, and oxidized-glutathione in leaves and roots, thereby impairing growth and metabolism. Furthermore, there is a significant accumulation of fructose and glucose, but lower sucrose and starch, possibly due to higher sucrose synthase, α-amylase, β-amylase and hexokinase but lower sucrose phosphate synthase and fructokinase. Drought-stressed seedlings also displayed higher abscisic and, jasmonic acids, strigolactones, glucose-6-phosphate dehydrogenase (G-6-PDH), phosphoenolpyruvate carboxylase, O<sub>2</sub><sup>•−</sup>-H<sub>2</sub>O<sub>2</sub>-scavenging enzymes, but lower gibberellin, cytokinin, and indole-acetic acid. However, N-addition quantifies the productivity of drought-stressed seedlings by improving the leaf relative water content (LRWC), biomass, chlorophyll-a, sucrose-synthesizing enzymes (SPP and SPS), and hormones. It also increased the G-6-PDH in stressed seedlings to satisfy the need for NADPH and reduced the sucrose and starch degrading enzymes, leading to higher starch and sucrose levels. Upregulation of O<sub>2</sub><sup>•−</sup>-H<sub>2</sub>O<sub>2</sub> -scavenging enzymes under N-supply reduced lipid peroxidation and improved the ascorbate–glutathione redox states. N addition might be an effective strategy to improve drought resistance in <i>A. sparsifolia</i> seedlings to manage and conserve its vegetation in hyper-arid conditions in the face of future climate change.</p>\",\"PeriodicalId\":17042,\"journal\":{\"name\":\"Journal of Soil Science and Plant Nutrition\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.4000,\"publicationDate\":\"2024-07-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Soil Science and Plant Nutrition\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1007/s42729-024-01814-w\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"ENVIRONMENTAL SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Soil Science and Plant Nutrition","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s42729-024-01814-w","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
引用次数: 0

摘要

成熟的旱生植物可以利用地下水,最大程度地降低干旱环境中缺水和缺养分的风险。然而,它们的幼苗在到达地下水之前是如何对水分和养分的可用性做出反应的,这在很大程度上还是个未知数。我们研究了不同干旱机制(受控、中度干旱(MD)和严重干旱(SD))]和氮(添加或不添加)对Alhagi sparsifolia幼苗生物量和生理生化反应的影响。两种干旱胁迫都会明显增加叶片和根部的超氧化物歧化酶(O2--)、过氧化氢(H2O2)、丙二醛和氧化谷胱甘肽,从而影响生长和新陈代谢。此外,果糖和葡萄糖显著积累,而蔗糖和淀粉则较低,这可能是由于蔗糖合成酶、α-淀粉酶、β-淀粉酶和己糖激酶较高,而磷酸蔗糖合成酶和果糖激酶较低所致。干旱胁迫秧苗还表现出较高的赤霉酸和茉莉酸、赤霉内酯、葡萄糖-6-磷酸脱氢酶(G-6-PDH)、磷酸烯醇丙酮酸羧化酶、O2--H2O2清除酶,但赤霉素、细胞分裂素和吲哚乙酸较低。然而,通过提高叶片相对含水量(LRWC)、生物量、叶绿素-a、蔗糖合成酶(SPP 和 SPS)和激素,添加氮可以量化干旱胁迫秧苗的生产力。它还增加了受胁迫幼苗中的 G-6-PDH 以满足对 NADPH 的需求,并减少了蔗糖和淀粉降解酶,从而提高了淀粉和蔗糖含量。在氮供应条件下,O2--H2O2清除酶的上调降低了脂质过氧化,改善了抗坏血酸-谷胱甘肽的氧化还原状态。面对未来的气候变化,添加氮可能是提高 A. sparsifolia幼苗抗旱性的有效策略,以管理和保护其在超干旱条件下的植被。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Insights into Alhagi sparsifolia Seedlings Adaptations to Drought Stress under Nitrogen Addition: Regulation of Sugar Metabolism, Hormones, and Anti-Oxidant Potential

Insights into Alhagi sparsifolia Seedlings Adaptations to Drought Stress under Nitrogen Addition: Regulation of Sugar Metabolism, Hormones, and Anti-Oxidant Potential

Mature xerophytes access groundwater and minimize the risk of water and nutrient deficits in arid environments. However, how their young seedlings respond to the availability of water and nutrients before they reach groundwater is largely unknown. We investigated the effects of different drought regimes (controlled, medium-drought (MD), and severe-drought (SD)] and nitrogen (N; with or without) addition on biomass and physio-biochemical responses in Alhagi sparsifolia seedlings. Both drought stresses significantly increased superoxide dismutase (O2•−), hydrogen peroxide (H2O2), malondialdehyde, and oxidized-glutathione in leaves and roots, thereby impairing growth and metabolism. Furthermore, there is a significant accumulation of fructose and glucose, but lower sucrose and starch, possibly due to higher sucrose synthase, α-amylase, β-amylase and hexokinase but lower sucrose phosphate synthase and fructokinase. Drought-stressed seedlings also displayed higher abscisic and, jasmonic acids, strigolactones, glucose-6-phosphate dehydrogenase (G-6-PDH), phosphoenolpyruvate carboxylase, O2•−-H2O2-scavenging enzymes, but lower gibberellin, cytokinin, and indole-acetic acid. However, N-addition quantifies the productivity of drought-stressed seedlings by improving the leaf relative water content (LRWC), biomass, chlorophyll-a, sucrose-synthesizing enzymes (SPP and SPS), and hormones. It also increased the G-6-PDH in stressed seedlings to satisfy the need for NADPH and reduced the sucrose and starch degrading enzymes, leading to higher starch and sucrose levels. Upregulation of O2•−-H2O2 -scavenging enzymes under N-supply reduced lipid peroxidation and improved the ascorbate–glutathione redox states. N addition might be an effective strategy to improve drought resistance in A. sparsifolia seedlings to manage and conserve its vegetation in hyper-arid conditions in the face of future climate change.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Journal of Soil Science and Plant Nutrition
Journal of Soil Science and Plant Nutrition Agricultural and Biological Sciences-Soil Science
CiteScore
5.90
自引率
10.30%
发文量
331
审稿时长
9 months
期刊介绍: The Journal of Soil Science and Plant Nutrition is an international, peer reviewed journal devoted to publishing original research findings in the areas of soil science, plant nutrition, agriculture and environmental science. Soil sciences submissions may cover physics, chemistry, biology, microbiology, mineralogy, ecology, pedology, soil classification and amelioration. Plant nutrition and agriculture submissions may include plant production, physiology and metabolism of plants, plant ecology, diversity and sustainability of agricultural systems, organic and inorganic fertilization in relation to their impact on yields, quality of plants and ecological systems, and agroecosystems studies. Submissions covering soil degradation, environmental pollution, nature conservation, and environmental protection are also welcome. The journal considers for publication original research articles, technical notes, short communication, and reviews (both voluntary and by invitation), and letters to the editor.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信