分析块鱼中的 Mx 家族成员:分子特征、系统发育和基因表达分析

IF 2.7 3区 农林科学 Q1 FISHERIES
{"title":"分析块鱼中的 Mx 家族成员:分子特征、系统发育和基因表达分析","authors":"","doi":"10.1016/j.dci.2024.105225","DOIUrl":null,"url":null,"abstract":"<div><p>Members of the myxovirus resistance (Mx) protein family play an essential role in antiviral immunity. They are Dynamin-like GTPases, induced by interferons. In the current study, we have characterized two predicted <em>MX</em> genes (<em>MX</em>1 and <em>MX</em>2) from lumpfish (<em>Cyclopterus lumpus</em> L.), having 12 and 13 exons, respectively. Mx2 has two isoforms (Mx2-X1 and Mx2-X2) which differ in exon 1. The lumpfish Mx proteins contain an N-terminal Dynamin-like GTPase domain, the middle domain (MD) and GTPase effector domain (GED) characteristic for Mx proteins. Phylogenetic analyses grouped all the lumpfish Mx sequences in group 1, and synteny analyses showed that both genes were localized at chromosome 5 in proximity to the genes Tohc7, Atxn7 and Psmd6. <em>In vitro</em> stimulation experiment showed that both <em>MX1</em> and <em>MX2</em>-X2 were highly upregulated upon exposure to poly(I:C), but not bacteria, 24 h post exposure, indicating their role in antiviral immunity.</p></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"159 ","pages":"Article 105225"},"PeriodicalIF":2.7000,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Analyses of the Mx family members in lumpfish: Molecular characterization, phylogeny, and gene expression analyses\",\"authors\":\"\",\"doi\":\"10.1016/j.dci.2024.105225\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Members of the myxovirus resistance (Mx) protein family play an essential role in antiviral immunity. They are Dynamin-like GTPases, induced by interferons. In the current study, we have characterized two predicted <em>MX</em> genes (<em>MX</em>1 and <em>MX</em>2) from lumpfish (<em>Cyclopterus lumpus</em> L.), having 12 and 13 exons, respectively. Mx2 has two isoforms (Mx2-X1 and Mx2-X2) which differ in exon 1. The lumpfish Mx proteins contain an N-terminal Dynamin-like GTPase domain, the middle domain (MD) and GTPase effector domain (GED) characteristic for Mx proteins. Phylogenetic analyses grouped all the lumpfish Mx sequences in group 1, and synteny analyses showed that both genes were localized at chromosome 5 in proximity to the genes Tohc7, Atxn7 and Psmd6. <em>In vitro</em> stimulation experiment showed that both <em>MX1</em> and <em>MX2</em>-X2 were highly upregulated upon exposure to poly(I:C), but not bacteria, 24 h post exposure, indicating their role in antiviral immunity.</p></div>\",\"PeriodicalId\":11228,\"journal\":{\"name\":\"Developmental and comparative immunology\",\"volume\":\"159 \",\"pages\":\"Article 105225\"},\"PeriodicalIF\":2.7000,\"publicationDate\":\"2024-07-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Developmental and comparative immunology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0145305X24000971\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"FISHERIES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Developmental and comparative immunology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0145305X24000971","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"FISHERIES","Score":null,"Total":0}
引用次数: 0

摘要

肌瘤病毒抗性(Mx)蛋白家族成员在抗病毒免疫中发挥着重要作用。它们是类似 Dynamin 的 GTP 酶,由干扰素诱导。在目前的研究中,我们鉴定了两种预测的囫囵鱼(Cyclopterus lumpus L.)MX 基因(MX1 和 MX2),它们分别有 12 和 13 个外显子。Mx2 有两种异构体(Mx2-X1 和 Mx2-X2),它们在外显子 1 上存在差异。块鱼 Mx 蛋白含有一个 N 端 Dynamin 样 GTPase 结构域、Mx 蛋白特有的中间结构域(MD)和 GTPase 效应结构域(GED)。系统进化分析将所有块鱼 Mx 序列归为第 1 组,同源分析表明这两个基因都位于 5 号染色体上,与 Tohc7、Atxn7 和 Psmd6 基因相邻。体外刺激实验表明,MX1和MX2-X2在暴露于poly(I:C)后24小时高度上调,而在暴露于细菌后24小时不上调,表明它们在抗病毒免疫中的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Analyses of the Mx family members in lumpfish: Molecular characterization, phylogeny, and gene expression analyses

Members of the myxovirus resistance (Mx) protein family play an essential role in antiviral immunity. They are Dynamin-like GTPases, induced by interferons. In the current study, we have characterized two predicted MX genes (MX1 and MX2) from lumpfish (Cyclopterus lumpus L.), having 12 and 13 exons, respectively. Mx2 has two isoforms (Mx2-X1 and Mx2-X2) which differ in exon 1. The lumpfish Mx proteins contain an N-terminal Dynamin-like GTPase domain, the middle domain (MD) and GTPase effector domain (GED) characteristic for Mx proteins. Phylogenetic analyses grouped all the lumpfish Mx sequences in group 1, and synteny analyses showed that both genes were localized at chromosome 5 in proximity to the genes Tohc7, Atxn7 and Psmd6. In vitro stimulation experiment showed that both MX1 and MX2-X2 were highly upregulated upon exposure to poly(I:C), but not bacteria, 24 h post exposure, indicating their role in antiviral immunity.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
6.20
自引率
6.90%
发文量
206
审稿时长
49 days
期刊介绍: Developmental and Comparative Immunology (DCI) is an international journal that publishes articles describing original research in all areas of immunology, including comparative aspects of immunity and the evolution and development of the immune system. Manuscripts describing studies of immune systems in both vertebrates and invertebrates are welcome. All levels of immunological investigations are appropriate: organismal, cellular, biochemical and molecular genetics, extending to such fields as aging of the immune system, interaction between the immune and neuroendocrine system and intestinal immunity.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信