R. Sridharan, P. S. Kumar, K. Veenagayathri, G. Rangasamy
{"title":"利用 Winogradsky 柱和城市固体废物中的细菌菌株预测低密度聚乙烯降解途径","authors":"R. Sridharan, P. S. Kumar, K. Veenagayathri, G. Rangasamy","doi":"10.1007/s13762-024-05866-4","DOIUrl":null,"url":null,"abstract":"<p>Plastic waste, considered a great threat to the environment, requires an effective treatment process. The ability of the microbes to oxidize the polymeric chain (C–C bonds), hydrolyze and produce carbon dioxide and water as final products of degradation was studied. The process becomes complicated due to structural complexity of the polymer. The present study is the continuation of the LDPE degradation using the Winogradsky Column. The determination of metabolites formed on degradation is discussed. The FTIR analysis indicated the reduction in the intensity of the C-H, confirming the cleavage of the alkane chains in LDPE. The metabolites produced during the degradation resulted in the formation of smaller alkanes, which contain C32, C22, C16, C18 and aromatic compounds such as phenols and benzene dicarboxylic acid. The occurrence of terminal oxidation of the polymeric chain, cleavage, fragmentation and cyclization of the alkanes confirm the biodegradation process. The current research also focuses on the biodegradation of LDPE using bacterial strains isolated from dumpsite soil samples. The degraded LDPE was analyzed for its metabolite production using GC–MS. It enabled us to understand and hypothesize an overview pathway of LDPE degradation by bacterial strains. The hypothesized pathway indicated that bacterial strains performed fragmentation and cyclization of the long polymeric chain, followed by hydrogenation and oxidation, resulting in the formation of alcohols, aldehydes, ketones and carboxylic acid compounds leading to ester formation. The esters are then understood to enter the ꞵ-oxidation pathway or TCA cycle, producing carbon dioxide and water molecules.</p>","PeriodicalId":589,"journal":{"name":"International Journal of Environmental Science and Technology","volume":null,"pages":null},"PeriodicalIF":3.0000,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Pathway prediction of LDPE degradation using Winogradsky column and bacterial strains from municipal solid wastes\",\"authors\":\"R. Sridharan, P. S. Kumar, K. Veenagayathri, G. Rangasamy\",\"doi\":\"10.1007/s13762-024-05866-4\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Plastic waste, considered a great threat to the environment, requires an effective treatment process. The ability of the microbes to oxidize the polymeric chain (C–C bonds), hydrolyze and produce carbon dioxide and water as final products of degradation was studied. The process becomes complicated due to structural complexity of the polymer. The present study is the continuation of the LDPE degradation using the Winogradsky Column. The determination of metabolites formed on degradation is discussed. The FTIR analysis indicated the reduction in the intensity of the C-H, confirming the cleavage of the alkane chains in LDPE. The metabolites produced during the degradation resulted in the formation of smaller alkanes, which contain C32, C22, C16, C18 and aromatic compounds such as phenols and benzene dicarboxylic acid. The occurrence of terminal oxidation of the polymeric chain, cleavage, fragmentation and cyclization of the alkanes confirm the biodegradation process. The current research also focuses on the biodegradation of LDPE using bacterial strains isolated from dumpsite soil samples. The degraded LDPE was analyzed for its metabolite production using GC–MS. It enabled us to understand and hypothesize an overview pathway of LDPE degradation by bacterial strains. The hypothesized pathway indicated that bacterial strains performed fragmentation and cyclization of the long polymeric chain, followed by hydrogenation and oxidation, resulting in the formation of alcohols, aldehydes, ketones and carboxylic acid compounds leading to ester formation. 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Pathway prediction of LDPE degradation using Winogradsky column and bacterial strains from municipal solid wastes
Plastic waste, considered a great threat to the environment, requires an effective treatment process. The ability of the microbes to oxidize the polymeric chain (C–C bonds), hydrolyze and produce carbon dioxide and water as final products of degradation was studied. The process becomes complicated due to structural complexity of the polymer. The present study is the continuation of the LDPE degradation using the Winogradsky Column. The determination of metabolites formed on degradation is discussed. The FTIR analysis indicated the reduction in the intensity of the C-H, confirming the cleavage of the alkane chains in LDPE. The metabolites produced during the degradation resulted in the formation of smaller alkanes, which contain C32, C22, C16, C18 and aromatic compounds such as phenols and benzene dicarboxylic acid. The occurrence of terminal oxidation of the polymeric chain, cleavage, fragmentation and cyclization of the alkanes confirm the biodegradation process. The current research also focuses on the biodegradation of LDPE using bacterial strains isolated from dumpsite soil samples. The degraded LDPE was analyzed for its metabolite production using GC–MS. It enabled us to understand and hypothesize an overview pathway of LDPE degradation by bacterial strains. The hypothesized pathway indicated that bacterial strains performed fragmentation and cyclization of the long polymeric chain, followed by hydrogenation and oxidation, resulting in the formation of alcohols, aldehydes, ketones and carboxylic acid compounds leading to ester formation. The esters are then understood to enter the ꞵ-oxidation pathway or TCA cycle, producing carbon dioxide and water molecules.
期刊介绍:
International Journal of Environmental Science and Technology (IJEST) is an international scholarly refereed research journal which aims to promote the theory and practice of environmental science and technology, innovation, engineering and management.
A broad outline of the journal''s scope includes: peer reviewed original research articles, case and technical reports, reviews and analyses papers, short communications and notes to the editor, in interdisciplinary information on the practice and status of research in environmental science and technology, both natural and man made.
The main aspects of research areas include, but are not exclusive to; environmental chemistry and biology, environments pollution control and abatement technology, transport and fate of pollutants in the environment, concentrations and dispersion of wastes in air, water, and soil, point and non-point sources pollution, heavy metals and organic compounds in the environment, atmospheric pollutants and trace gases, solid and hazardous waste management; soil biodegradation and bioremediation of contaminated sites; environmental impact assessment, industrial ecology, ecological and human risk assessment; improved energy management and auditing efficiency and environmental standards and criteria.