Małgorzata Grzyb, Wojciech Tomaszewicz, Monika Cioć, Bożena Pawłowska, Anna Mikuła
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引用次数: 0
摘要
本研究评估了温度以及发光二极管(LED)和荧光灯(Fl)发出的不同光质对蕨类植物桫椤(Cyathea delgadii)微繁殖的影响。在 22 °C 的温度下,节间外植体获得了最有效的体细胞胚胎。获得发育良好植株的最佳温度范围为 24-28 °C。这刺激了第一片叶子的伸长和发育,以及下一片叶子和根系原基的形成。温度低于 24 ℃ 和高于 28 ℃ 会抑制幼孢子体器官的形成并延迟其发育。RBUV(35% 红光、15% 蓝光和 50% 紫外光)和 B(100% 蓝光)光以及 Fl 光有利于节间外植体上孢子体的形成。但在 RBUV 光下获得的植株不发达。白色 LED 光能刺激配子体产生和发育的外植体数量。RB 光(70% 红光和 30% 蓝光)增加了新形成植株的根系数量。与 Fl 光和恒定黑暗相比,大多数测试的 LED 光对根的伸长都有很好的影响。R 光(100% 红光)有利于叶片的发育和伸长。研究表明,温度和 LED 光在 C. delgadii 的形态发生过程中起着重要作用,显著影响体细胞的胚胎发生能力和孢子体的发育。
Temperature and LED lightning affect the regeneration of somatic embryo-derived sporophytes on the internode explants of the tree fern Cyathea delgadii Sternb.
The influence of the temperature and different light qualities emitted by light-emitting diodes (LEDs) and fluorescent lamps (Fl) on the micropropagation of the tree fern Cyathea delgadii was evaluated. The most efficient somatic embryo production was obtained on internode explants at 22 °C. The optimal temperature range for obtaining well-developed plants was 24–28 °C. This stimulated the elongation and development of the first leaf and the formation of the next leaf and roots primordia. Temperatures lower than 24 °C and higher than 28 °C inhibited the formation of young sporophyte organs and delayed their development. The RBUV (35% red, 15% blue, and 50% UV) and B (100% blue) lights and Fl light were beneficial for the sporophyte production on internode explants. However, plants obtained under RBUV light were undeveloped. The white LED light stimulated the number of explants capable of gametophyte production and development. The RB light (70% red and 30% blue) enhanced the number of roots of newly-formed plants. Most of the LED lights tested had a good impact on root elongation compared to Fl light and constant darkness. The R light (100% red) benefits leaf development and elongation. Research shows that temperature and LED lightning play a significant role in the process of morphogenesis in C. delgadii, significantly affecting the embryogenic competence of somatic cells and the development of sporophytes.
期刊介绍:
This journal highlights the myriad breakthrough technologies and discoveries in plant biology and biotechnology. Plant Cell, Tissue and Organ Culture (PCTOC: Journal of Plant Biotechnology) details high-throughput analysis of gene function and expression, gene silencing and overexpression analyses, RNAi, siRNA, and miRNA studies, and much more. It examines the transcriptional and/or translational events involved in gene regulation as well as those molecular controls involved in morphogenesis of plant cells and tissues.
The journal also covers practical and applied plant biotechnology, including regeneration, organogenesis and somatic embryogenesis, gene transfer, gene flow, secondary metabolites, metabolic engineering, and impact of transgene(s) dissemination into managed and unmanaged plant systems.