{"title":"研究百日草提取物的抗菌特性以及从 Fe-MOF 增强明胶基水凝胶中的持续释放性能","authors":"Sepideh Hezari, Ali Olad, Azita Dilmaghani","doi":"10.1007/s00289-024-05416-5","DOIUrl":null,"url":null,"abstract":"<p>The goal of this study is to investigate the antibacterial activity and sustained release of Centella Asiatica (CA) extract loaded in gelatin-based hydrogels, reinforced with MIL-53(Fe) (Fe-MOF) for the potential use as wound dressing. To induce antibacterial activity to the designed wound dressing, CA-extract was incorporated within the nanocomposite hydrogels. FT-IR analysis showed the distinctive peaks related to the Fe-MOF and CA-extract, with little shift, for hydrogel samples, which confirm the successful synthesis of nanocomposite hydrogels containing Fe-MOF and CA-extract. GC-mass analysis showed the antibacterial potential for CA-extract because of the presence of phenol, caryophyllene, and propanoic acid. SEM micrograph demonstrates the presence of Fe-MOF enhancing the porous of hydrogels. The antibacterial activity of prepared nanocomposite hydrogels was investigated by disk diffusion method revealing the significant inhibition zone growth for <i>Basillus</i>, <i>Staphylococcus</i>, and <i>Streptococcus</i>, as gram-positive and <i>E. coli</i>, <i>Klebsiella</i>, and <i>P. aeruginosa</i> as gram-negative bacteria and the \"<i>Candida albicans</i> as fungi. Also, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by the micro-broth dilution method. The results showed that the nanocomposite hydrogel has a MIC values of 3.125 mg/ml and 6.25 mg/ml against both groups of bacteria. Cytotoxicity results obtained from MTT tests showed that the prepared hydrogels are biocompatible. 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To induce antibacterial activity to the designed wound dressing, CA-extract was incorporated within the nanocomposite hydrogels. FT-IR analysis showed the distinctive peaks related to the Fe-MOF and CA-extract, with little shift, for hydrogel samples, which confirm the successful synthesis of nanocomposite hydrogels containing Fe-MOF and CA-extract. GC-mass analysis showed the antibacterial potential for CA-extract because of the presence of phenol, caryophyllene, and propanoic acid. SEM micrograph demonstrates the presence of Fe-MOF enhancing the porous of hydrogels. The antibacterial activity of prepared nanocomposite hydrogels was investigated by disk diffusion method revealing the significant inhibition zone growth for <i>Basillus</i>, <i>Staphylococcus</i>, and <i>Streptococcus</i>, as gram-positive and <i>E. coli</i>, <i>Klebsiella</i>, and <i>P. aeruginosa</i> as gram-negative bacteria and the \\\"<i>Candida albicans</i> as fungi. 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引用次数: 0
摘要
本研究的目的是调查积雪草(CA)提取物在明胶基水凝胶中的抗菌活性和持续释放情况,该水凝胶由 MIL-53(Fe)(Fe-MOF)增强,可用作伤口敷料。为了使设计的伤口敷料具有抗菌活性,CA 提取物被加入到纳米复合水凝胶中。傅立叶变换红外光谱分析显示,在水凝胶样品中,Fe-MOF 和 CA 提取物的相关峰值变化很小,这证实了含有 Fe-MOF 和 CA 提取物的纳米复合水凝胶的成功合成。气相色谱-质谱分析表明 CA 提取物具有抗菌潜力,因为其中含有苯酚、茶碱和丙酸。SEM 显微照片显示,Fe-MOF 的存在增强了水凝胶的多孔性。通过磁盘扩散法研究了制备的纳米复合水凝胶的抗菌活性,结果表明其对革兰氏阳性菌巴希氏菌、葡萄球菌和链球菌,革兰氏阴性菌大肠杆菌、克雷伯氏菌和铜绿假单胞菌以及真菌白色念珠菌都有显著的抑制作用。此外,还采用微滴稀释法测定了最低抑菌浓度(MIC)和最低杀菌浓度(MBC)。结果显示,纳米复合水凝胶对两类细菌的 MIC 值分别为 3.125 毫克/毫升和 6.25 毫克/毫升。MTT 试验的细胞毒性结果表明,制备的水凝胶具有生物相容性。免疫组化染色的水凝胶图像显示,与纯水凝胶相比,细胞在含有 CA 提取物的水凝胶上的生长速度明显加快。
Investigation of antibacterial properties and sustained release of Centella Asiatica extract from Fe-MOF-reinforced gelatin-based hydrogels
The goal of this study is to investigate the antibacterial activity and sustained release of Centella Asiatica (CA) extract loaded in gelatin-based hydrogels, reinforced with MIL-53(Fe) (Fe-MOF) for the potential use as wound dressing. To induce antibacterial activity to the designed wound dressing, CA-extract was incorporated within the nanocomposite hydrogels. FT-IR analysis showed the distinctive peaks related to the Fe-MOF and CA-extract, with little shift, for hydrogel samples, which confirm the successful synthesis of nanocomposite hydrogels containing Fe-MOF and CA-extract. GC-mass analysis showed the antibacterial potential for CA-extract because of the presence of phenol, caryophyllene, and propanoic acid. SEM micrograph demonstrates the presence of Fe-MOF enhancing the porous of hydrogels. The antibacterial activity of prepared nanocomposite hydrogels was investigated by disk diffusion method revealing the significant inhibition zone growth for Basillus, Staphylococcus, and Streptococcus, as gram-positive and E. coli, Klebsiella, and P. aeruginosa as gram-negative bacteria and the "Candida albicans as fungi. Also, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by the micro-broth dilution method. The results showed that the nanocomposite hydrogel has a MIC values of 3.125 mg/ml and 6.25 mg/ml against both groups of bacteria. Cytotoxicity results obtained from MTT tests showed that the prepared hydrogels are biocompatible. The immunohistochemistry-stained hydrogel images revealed that the growth of cells on the CA-extract containing hydrogels was significantly increased in comparison to neat hydrogels.
期刊介绍:
"Polymer Bulletin" is a comprehensive academic journal on polymer science founded in 1988. It was founded under the initiative of the late Mr. Wang Baoren, a famous Chinese chemist and educator. This journal is co-sponsored by the Chinese Chemical Society, the Institute of Chemistry, and the Chinese Academy of Sciences and is supervised by the China Association for Science and Technology. It is a core journal and is publicly distributed at home and abroad.
"Polymer Bulletin" is a monthly magazine with multiple columns, including a project application guide, outlook, review, research papers, highlight reviews, polymer education and teaching, information sharing, interviews, polymer science popularization, etc. The journal is included in the CSCD Chinese Science Citation Database. It serves as the source journal for Chinese scientific and technological paper statistics and the source journal of Peking University's "Overview of Chinese Core Journals."