基于超高效液相色谱-二极管阵列检测器指纹图谱和多组分超高效液相色谱-串联质谱技术的藿香正气丸质量控制综合策略。

IF 2.8 3区 工程技术 Q2 CHEMISTRY, ANALYTICAL
Yang Lu, Ting Lei, Xinyang Chen, Na Ning, Qiuling Huang, Xu Wu, Shengpeng Wang, Peng Li, Li Wan, Jiliang Cao
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引用次数: 0

摘要

加味藿香正气丸(JHZP)是临床治疗风寒感冒引起的头痛、头晕、胸闷、腹胀、疼痛的常用中成药。本研究建立了超高效液相色谱-二极管阵列检测器(UHPLC-DAD)指纹图谱和多组分定量分析相结合的综合策略,并对其进行了质量评价和验证。在色谱指纹图谱研究中,共选择了 49 个特征共用峰,以评估 15 批 JHZP 的相似性。此外,通过与先进的混合线性离子阱-轨道阱质谱仪联用,从 JHZP 中鉴定或初步鉴定出 109 种化合物。在定量分析方面,采用优化的超高效液相色谱-串联质谱(UPLC-MS/MS)方法,在12分钟内同时测定了13种目标化合物。该方法的灵敏度、精密度、重现性和准确度均令人满意。该UPLC-MS/MS方法已成功应用于15批次JHZP的分析。该方法结合了超高效液相色谱-DAD指纹图谱和多组分UPLC-MS/MS分析技术,对金银花的质量评价具有高效、准确、可靠的特点,可为其他中药配方的质量评价提供参考。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A comprehensive strategy based on ultra-high performance liquid chromatography with diode array detector fingerprinting and multi-component ultra-performance liquid chromatography with tandem mass spectrometry technology for quality control of Jiawei Huoxiang Zhengqi Pill

Jiawei Huoxiang Zhengqi Pill (JHZP) is a commonly used Chinese patent medicine for the clinical treatment of headache, dizziness, chest tightness as well as abdominal distension, and pain caused by wind-cold flu. In this study, a comprehensive strategy combining ultra-high performance liquid chromatography with diode array detector (UHPLC-DAD) fingerprinting and multi-component quantitative analysis was established and validated for quality evaluation of JHZP. A total of 49 characteristic common peaks were selected in a chromatographic fingerprinting study to assess the similarity of 15 batches of JHZP. Furthermore, 109 compounds were identified or preliminarily identified from JHZP by coupling with an advanced hybrid linear ion trap-Orbitrap mass spectrometer. For quantification, the optimized ultra-performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS) method was employed for the simultaneous determination of 13 target compounds within 12 min. The sensitivity, precision, reproducibility, and accuracy of the method were satisfactory. This validated UPLC-MS/MS method was successfully applied to analyzing 15 batches of JHZP. The proposed comprehensive strategy combining UHPLC-DAD fingerprinting and multi-component UPLC-MS/MS analysis proved to be highly efficient, accurate, and reliable for the quality evaluation of JHZP, which can be considered as a reference for the overall quality evaluation of other Chinese herbal formulations.

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来源期刊
Journal of separation science
Journal of separation science 化学-分析化学
CiteScore
6.30
自引率
16.10%
发文量
408
审稿时长
1.8 months
期刊介绍: The Journal of Separation Science (JSS) is the most comprehensive source in separation science, since it covers all areas of chromatographic and electrophoretic separation methods in theory and practice, both in the analytical and in the preparative mode, solid phase extraction, sample preparation, and related techniques. Manuscripts on methodological or instrumental developments, including detection aspects, in particular mass spectrometry, as well as on innovative applications will also be published. Manuscripts on hyphenation, automation, and miniaturization are particularly welcome. Pre- and post-separation facets of a total analysis may be covered as well as the underlying logic of the development or application of a method.
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