利用钙培养液培养深海沉积物中的多种新型海洋细菌Bathyarchaeia Enrichment.

IF 3.3 4区 生物学 Q2 MICROBIOLOGY
Journal of Microbiology Pub Date : 2024-08-01 Epub Date: 2024-07-10 DOI:10.1007/s12275-024-00145-w
Sidra Erum Ishaq, Tariq Ahmad, Lewen Liang, Ruize Xie, Tiantian Yu, Yinzhao Wang, Fengping Wang
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引用次数: 0

摘要

大多数微生物都无法在传统实验室条件下进行纯培养。造成这种不可培养性的主要问题之一是,在传统的生长培养基中缺乏由生物产生的生长促进因子。然而,通过在生长培养基中提供关键微生物的废培养上清液来培养微生物能否成为克服这一限制的有效方法,目前仍是一个探索不足的研究领域。在此,我们使用乏培养基(SCM)方法分离了以前未培养过的海洋细菌,并比较了该方法与传统培养(TC)方法的效率。在 SCM 法中,Ca.Bathyarchaeia 富集的上清液(10%)以及木质素、腐殖酸和有机碳混合物等难降解的有机基质。Ca.蝙蝠弓形虫是一类无处不在的古细菌,具有产生代谢物的能力,因此它们的废培养上清液是恢复新细菌染色的关键来源。这两种培养方法都回收了假单胞菌门、类杆菌门、放线菌门和芽孢杆菌门的细菌物种。不过,我们的单片机方法也能从很少培养的类群中回收菌种,如 Planctomycetota、Deinococcota 和 Balneolota。在新类群的分离方面,单克隆法培育出了 80 个潜在的新菌株,其中包括 1 个科、16 个属和 63 个种,新菌株比率约为 35%(80/219)。相比之下,TC 方法只能分离出约 10%(19/171)的种级新菌株。这些研究结果表明,单片机方法提高了新型和多样化细菌的培养效果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Cultivation of Diverse Novel Marine Bacteria from Deep Ocean Sediment Using Spent Culture Supernatant of Ca. Bathyarchaeia Enrichment.

Cultivation of Diverse Novel Marine Bacteria from Deep Ocean Sediment Using Spent Culture Supernatant of Ca. Bathyarchaeia Enrichment.

Most microorganisms resist pure cultivation under conventional laboratory conditions. One of the primary issues for this un-culturability is the absence of biologically produced growth-promoting factors in traditionally defined growth media. However, whether cultivating microbes by providing spent culture supernatant of pivotal microbes in the growth medium can be an effective approach to overcome this limitation is still an under-explored area of research. Here, we used the spent culture medium (SCM) method to isolate previously uncultivated marine bacteria and compared the efficiency of this method with the traditional cultivation (TC) method. In the SCM method, Ca. Bathyarchaeia-enriched supernatant (10%) was used along with recalcitrant organic substrates such as lignin, humic acid, and organic carbon mixture. Ca. Bathyarchaeia, a ubiquitous class of archaea, have the capacity to produce metabolites, making their spent culture supernatant a key source to recover new bacterial stains. Both cultivation methods resulted in the recovery of bacterial species from the phyla Pseudomonadota, Bacteroidota, Actinomycetota, and Bacillota. However, our SCM approach also led to the recovery of species from rarely cultivated groups, such as Planctomycetota, Deinococcota, and Balneolota. In terms of the isolation of new taxa, the SCM method resulted in the cultivation of 80 potential new strains, including one at the family, 16 at the genus, and 63 at the species level, with a novelty ratio of ~ 35% (80/219). In contrast, the TC method allowed the isolation of ~ 10% (19/171) novel strains at species level only. These findings suggest that the SCM approach improved the cultivation of novel and diverse bacteria.

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来源期刊
Journal of Microbiology
Journal of Microbiology 生物-微生物学
CiteScore
5.70
自引率
3.30%
发文量
0
审稿时长
3 months
期刊介绍: Publishes papers that deal with research on microorganisms, including archaea, bacteria, yeasts, fungi, microalgae, protozoa, and simple eukaryotic microorganisms. Topics considered for publication include Microbial Systematics, Evolutionary Microbiology, Microbial Ecology, Environmental Microbiology, Microbial Genetics, Genomics, Molecular Biology, Microbial Physiology, Biochemistry, Microbial Pathogenesis, Host-Microbe Interaction, Systems Microbiology, Synthetic Microbiology, Bioinformatics and Virology. Manuscripts dealing with simple identification of microorganism(s), cloning of a known gene and its expression in a microbial host, and clinical statistics will not be considered for publication by JM.
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