大肠杆菌的 DNA 复制:这一古老的模式生物仍有许多惊喜。

IF 10.1 2区 生物学 Q1 MICROBIOLOGY
Krystian Łazowski, Roger Woodgate, Iwona J Fijalkowska
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引用次数: 0

摘要

对大肠杆菌 DNA 复制的研究为许多突破性发现奠定了基础,这些发现对我们了解人类分子生物学具有重要意义,因为其中涉及的关键分子过程高度保持不变。时至今日,大肠杆菌仍备受关注,一方面是因为它是一种重要的模式生物,另一方面也是因为了解影响复制保真度的因素可能对抗生素耐药性的产生具有重要意义。重要的是,高分辨率单分子和活细胞成像、全基因组测序以及低温电子显微镜技术在过去十年中得到了广泛应用,这使我们能够重新审视关于复制体的某些假设,并对它们的工作原理有了非常详细的了解。对于复制体的许多部分,我们已经重建了其逐步运行的机制,并发现了一些新的参与者。本综述总结了这一领域的最新进展,重点关注:(a) 复制体的结构及其各组成部分的作用机制;(b) 复制体交易和修复的组织;(c) 复制体的动态;(d) 影响 DNA 合成的碱基和糖保真度的因素。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Escherichia coli DNA replication: the old model organism still holds many surprises.

Research on Escherichia coli DNA replication paved the groundwork for many breakthrough discoveries with important implications for our understanding of human molecular biology, due to the high level of conservation of key molecular processes involved. To this day, it attracts a lot of attention, partially by virtue of being an important model organism, but also because the understanding of factors influencing replication fidelity might be important for studies on the emergence of antibiotic resistance. Importantly, the wide access to high-resolution single-molecule and live-cell imaging, whole genome sequencing, and cryo-electron microscopy techniques, which were greatly popularized in the last decade, allows us to revisit certain assumptions about the replisomes and offers very detailed insight into how they work. For many parts of the replisome, step-by-step mechanisms have been reconstituted, and some new players identified. This review summarizes the latest developments in the area, focusing on (a) the structure of the replisome and mechanisms of action of its components, (b) organization of replisome transactions and repair, (c) replisome dynamics, and (d) factors influencing the base and sugar fidelity of DNA synthesis.

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来源期刊
FEMS microbiology reviews
FEMS microbiology reviews 生物-微生物学
CiteScore
17.50
自引率
0.90%
发文量
45
审稿时长
6-12 weeks
期刊介绍: Title: FEMS Microbiology Reviews Journal Focus: Publishes reviews covering all aspects of microbiology not recently surveyed Reviews topics of current interest Provides comprehensive, critical, and authoritative coverage Offers new perspectives and critical, detailed discussions of significant trends May contain speculative and selective elements Aimed at both specialists and general readers Reviews should be framed within the context of general microbiology and biology Submission Criteria: Manuscripts should not be unevaluated compilations of literature Lectures delivered at symposia must review the related field to be acceptable
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