Eliana V. Machin, Diego M. Roldán, Rodolfo Javier Menes
{"title":"从河水中分离出的一种新型丝状细菌 Sphaerotilus uruguayifluvii sp.","authors":"Eliana V. Machin, Diego M. Roldán, Rodolfo Javier Menes","doi":"10.1007/s10482-024-01996-4","DOIUrl":null,"url":null,"abstract":"<div><p>Strain C29<sup>T</sup>, a Gram-staining-negative, straight rod occurring singly, in pairs or short chains, was isolated from floating filamentous biomass of the Uruguay River. The strain was catalase and oxidase positive, chemoorganotrophic, strictly aerobic, non-motile, and grew at pH 6.0–9.0, 15–45 °C, and 0–0.5% (w/v) NaCl. Polyhydroxybutyrate was accumulated in nutrient-limited conditions. Phylogenetic analysis based on the 16S rRNA gene revealed that strain C29<sup>T</sup> had the highest sequence similarity with <i>Leptothrix discophora</i> SS-1<sup>T</sup> (97.82%), <i>Ideonella livida</i> TBM-1<sup>T</sup> (97.82%), <i>Vitreoscilla filiformis</i> L1401-2<sup>T</sup> (97.52%), <i>Sphaerotilus sulfidivorans</i> D-501<sup>T</sup> (97.50%) and <i>Sphaerotilus natans</i> DSM 6575<sup>T</sup> (97.46%). Other type strains with validly published names had similarities below 97.46%. Further phylogenomic analysis showed that strain C29<sup>T</sup> was affiliated to the family <i>Sphaerotilaceae</i>. Average nucleotide identity (ANI) and in silico DNA–DNA hybridization (dDDH) values with its phylogenetic relatives were lower than 91 and 41%, respectively, revealing that strain C29<sup>T</sup> represented a new species. The DNA G + C content of strain C29<sup>T</sup> was 70.9%. The annotation of the genome of the novel strain shows it possessed genes for the degradation of aromatic compounds. It also contained genes that encode sigma factors involved in response regulation of stress resistance, which is an important function for adaptation and survival in natural niches. Based on the results of the phylogenetic and phenotypic analyses, we propose that strain C29<sup>T</sup> represents a novel species, for which the name <i>Sphaerotilus uruguayifluvii</i> sp. nov. is proposed. The type strain is C29<sup>T</sup> (= CCM 9043<sup>T</sup> = DSM 113250<sup>T</sup>).</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"117 1","pages":""},"PeriodicalIF":1.8000,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Sphaerotilus uruguayifluvii sp. nov., a novel filamentous bacterium isolated from river water\",\"authors\":\"Eliana V. Machin, Diego M. Roldán, Rodolfo Javier Menes\",\"doi\":\"10.1007/s10482-024-01996-4\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Strain C29<sup>T</sup>, a Gram-staining-negative, straight rod occurring singly, in pairs or short chains, was isolated from floating filamentous biomass of the Uruguay River. The strain was catalase and oxidase positive, chemoorganotrophic, strictly aerobic, non-motile, and grew at pH 6.0–9.0, 15–45 °C, and 0–0.5% (w/v) NaCl. Polyhydroxybutyrate was accumulated in nutrient-limited conditions. Phylogenetic analysis based on the 16S rRNA gene revealed that strain C29<sup>T</sup> had the highest sequence similarity with <i>Leptothrix discophora</i> SS-1<sup>T</sup> (97.82%), <i>Ideonella livida</i> TBM-1<sup>T</sup> (97.82%), <i>Vitreoscilla filiformis</i> L1401-2<sup>T</sup> (97.52%), <i>Sphaerotilus sulfidivorans</i> D-501<sup>T</sup> (97.50%) and <i>Sphaerotilus natans</i> DSM 6575<sup>T</sup> (97.46%). Other type strains with validly published names had similarities below 97.46%. Further phylogenomic analysis showed that strain C29<sup>T</sup> was affiliated to the family <i>Sphaerotilaceae</i>. Average nucleotide identity (ANI) and in silico DNA–DNA hybridization (dDDH) values with its phylogenetic relatives were lower than 91 and 41%, respectively, revealing that strain C29<sup>T</sup> represented a new species. The DNA G + C content of strain C29<sup>T</sup> was 70.9%. The annotation of the genome of the novel strain shows it possessed genes for the degradation of aromatic compounds. It also contained genes that encode sigma factors involved in response regulation of stress resistance, which is an important function for adaptation and survival in natural niches. Based on the results of the phylogenetic and phenotypic analyses, we propose that strain C29<sup>T</sup> represents a novel species, for which the name <i>Sphaerotilus uruguayifluvii</i> sp. nov. is proposed. The type strain is C29<sup>T</sup> (= CCM 9043<sup>T</sup> = DSM 113250<sup>T</sup>).</p></div>\",\"PeriodicalId\":50746,\"journal\":{\"name\":\"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology\",\"volume\":\"117 1\",\"pages\":\"\"},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2024-07-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s10482-024-01996-4\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1007/s10482-024-01996-4","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Sphaerotilus uruguayifluvii sp. nov., a novel filamentous bacterium isolated from river water
Strain C29T, a Gram-staining-negative, straight rod occurring singly, in pairs or short chains, was isolated from floating filamentous biomass of the Uruguay River. The strain was catalase and oxidase positive, chemoorganotrophic, strictly aerobic, non-motile, and grew at pH 6.0–9.0, 15–45 °C, and 0–0.5% (w/v) NaCl. Polyhydroxybutyrate was accumulated in nutrient-limited conditions. Phylogenetic analysis based on the 16S rRNA gene revealed that strain C29T had the highest sequence similarity with Leptothrix discophora SS-1T (97.82%), Ideonella livida TBM-1T (97.82%), Vitreoscilla filiformis L1401-2T (97.52%), Sphaerotilus sulfidivorans D-501T (97.50%) and Sphaerotilus natans DSM 6575T (97.46%). Other type strains with validly published names had similarities below 97.46%. Further phylogenomic analysis showed that strain C29T was affiliated to the family Sphaerotilaceae. Average nucleotide identity (ANI) and in silico DNA–DNA hybridization (dDDH) values with its phylogenetic relatives were lower than 91 and 41%, respectively, revealing that strain C29T represented a new species. The DNA G + C content of strain C29T was 70.9%. The annotation of the genome of the novel strain shows it possessed genes for the degradation of aromatic compounds. It also contained genes that encode sigma factors involved in response regulation of stress resistance, which is an important function for adaptation and survival in natural niches. Based on the results of the phylogenetic and phenotypic analyses, we propose that strain C29T represents a novel species, for which the name Sphaerotilus uruguayifluvii sp. nov. is proposed. The type strain is C29T (= CCM 9043T = DSM 113250T).
期刊介绍:
Antonie van Leeuwenhoek publishes papers on fundamental and applied aspects of microbiology. Topics of particular interest include: taxonomy, structure & development; biochemistry & molecular biology; physiology & metabolic studies; genetics; ecological studies; especially molecular ecology; marine microbiology; medical microbiology; molecular biological aspects of microbial pathogenesis and bioinformatics.