Olga Plisko, Jana Žodžika, Irina Jermakova, Inta Liepniece-Karele, Jeļena Eglīte, Dace Rezeberga
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The aim of the study was to assess an association between HLA-DQA1*; DQB1*; DRB1* allele's genetic variants between women with cervical precancerous lesions and healthy controls in Latvia.</p><p><strong>Materials and methods: </strong>From January until April 2017 we enrolled 84 consecutive patients referred for colposcopy to Riga East University Hospital (Latvia) due to abnormal cervical cytology results. 57 women who came for a regular check-up and had normal cytology smears were included in the control group. Material from the cervix was taken for subsequent HLA genotyping of 13 DRB1*, 8 DQA1*, and 12 DQB1* alleles. Colposcopy was performed on all participants. In case of visual suspicion for CIN cervical biopsy was done.</p><p><strong>Results: </strong>There were 57 \"no CIN\" patients, 23 histologically proven CIN 1 and 61 CIN2+ cases in the study population. CIN2+ was more often associated with DQA1*0401 (OR 6.68, 95% CI 1.47-30.29, p=0.014), DRB*15 (OR 2.99, 95% CI 1.22-7.39, p=0.017), DQB1*0401 (OR 2.91, 95%CI 1.11-7.68, p=0.03), DQA1*0103 (OR 2.72, 95% CI 1.02-7.21, p=0.045), DRB1*11 (OR 2.42, 95% CI 1.10-5.33, p=0.029) and DQB1*0301 (OR 1.94, 95% CI 1.12-3.38, p=0.018). Women with \"no CIN\" more often had DQB1*0501 (OR 0.17, 95% CI 0.04-0.81, p=0.026), DRB1*16 (OR 0.21, 95% CI 0.06-0.78, p=0.019), DQA1*0301 (OR 0.35, 95% CI 0.14-0.87, p=0.024) and DRB1*14 (OR 0.59, 95% CI 0.01-0.46, p=0.007).</p><p><strong>Conclusions: </strong>In the current study we have demonstrated a strong association with risk and protective HLA class II alleles that are determined by the HLA-DRB1*; DQA1*; DQB1*.</p>","PeriodicalId":34365,"journal":{"name":"Acta Medica Lituanica","volume":"31 1","pages":"5-11"},"PeriodicalIF":0.0000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11227681/pdf/","citationCount":"0","resultStr":"{\"title\":\"Human Leucocyte Antigen Class II Risk and Protective Alleles in Women with Cervical Intraepithelial Neoplasia.\",\"authors\":\"Olga Plisko, Jana Žodžika, Irina Jermakova, Inta Liepniece-Karele, Jeļena Eglīte, Dace Rezeberga\",\"doi\":\"10.15388/Amed.2024.31.1.1\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Persistent human papillomavirus (HPV) infection is a necessary cause for development of cervical precancerous lesions and cervical cancer, however, only a small percentage of women progress to cervical cancer. The local immune response, determined, among other factors, by Human Leucocyte Antigen (HLA) genes, is thought to be significant. Still the results of genome studies are inconsistent and differ between ethnical populations. The aim of the study was to assess an association between HLA-DQA1*; DQB1*; DRB1* allele's genetic variants between women with cervical precancerous lesions and healthy controls in Latvia.</p><p><strong>Materials and methods: </strong>From January until April 2017 we enrolled 84 consecutive patients referred for colposcopy to Riga East University Hospital (Latvia) due to abnormal cervical cytology results. 57 women who came for a regular check-up and had normal cytology smears were included in the control group. Material from the cervix was taken for subsequent HLA genotyping of 13 DRB1*, 8 DQA1*, and 12 DQB1* alleles. Colposcopy was performed on all participants. In case of visual suspicion for CIN cervical biopsy was done.</p><p><strong>Results: </strong>There were 57 \\\"no CIN\\\" patients, 23 histologically proven CIN 1 and 61 CIN2+ cases in the study population. CIN2+ was more often associated with DQA1*0401 (OR 6.68, 95% CI 1.47-30.29, p=0.014), DRB*15 (OR 2.99, 95% CI 1.22-7.39, p=0.017), DQB1*0401 (OR 2.91, 95%CI 1.11-7.68, p=0.03), DQA1*0103 (OR 2.72, 95% CI 1.02-7.21, p=0.045), DRB1*11 (OR 2.42, 95% CI 1.10-5.33, p=0.029) and DQB1*0301 (OR 1.94, 95% CI 1.12-3.38, p=0.018). 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引用次数: 0
摘要
背景：人类乳头瘤病毒（HPV）的持续感染是宫颈癌前病变和宫颈癌发生的必要原因，但只有一小部分妇女会发展为宫颈癌。除其他因素外，由人类白细胞抗原（HLA）基因决定的局部免疫反应被认为具有重要意义。不过，基因组研究的结果并不一致，而且不同种族的研究结果也不尽相同。本研究旨在评估拉脱维亚宫颈癌前病变妇女与健康对照组之间 HLA-DQA1*; DQB1*; DRB1* 等位基因遗传变异之间的关联：从 2017 年 1 月到 4 月，我们连续招募了 84 名因宫颈细胞学结果异常而转诊到里加东大医院（拉脱维亚）进行阴道镜检查的患者。57名前来进行定期检查且细胞学涂片结果正常的妇女被纳入对照组。从宫颈取材，随后对 13 个 DRB1*、8 个 DQA1* 和 12 个 DQB1* 等位基因进行 HLA 基因分型。所有参与者都接受了阴道镜检查。如果肉眼怀疑有 CIN，则进行宫颈活检：结果：研究人群中有 57 例 "无 CIN "患者，23 例经组织学证实的 CIN 1，61 例 CIN2+。CIN2+多与下列因素有关：DQA1*0401（OR 6.68，95% CI 1.47-30.29，p=0.014）、DRB*15（OR 2.99，95% CI 1.22-7.39，p=0.017）、DQB1*0401（OR 2.91，95%CI 1.11-7.68，p=0.03）、DQA1*0103（OR 2.72，95% CI 1.02-7.21，p=0.045）、DRB1*11（OR 2.42，95% CI 1.10-5.33，p=0.029）和 DQB1*0301（OR 1.94，95% CI 1.12-3.38，p=0.018）。而 "无CIN "的女性中，DQB1*0501（OR 0.17，95% CI 0.04-0.81，p=0.026）、DRB1*16（OR 0.21，95% CI 0.06-0.78，p=0.019）、DQA1*0301（OR 0.35，95% CI 0.14-0.87，p=0.024）和DRB1*14（OR 0.59，95% CI 0.01-0.46，p=0.007）更常见：在目前的研究中，我们已经证明了由 HLA-DRB1*; DQA1*; DQB1* 决定的风险性和保护性 HLA II 类等位基因之间的密切联系。
Human Leucocyte Antigen Class II Risk and Protective Alleles in Women with Cervical Intraepithelial Neoplasia.
Background: Persistent human papillomavirus (HPV) infection is a necessary cause for development of cervical precancerous lesions and cervical cancer, however, only a small percentage of women progress to cervical cancer. The local immune response, determined, among other factors, by Human Leucocyte Antigen (HLA) genes, is thought to be significant. Still the results of genome studies are inconsistent and differ between ethnical populations. The aim of the study was to assess an association between HLA-DQA1*; DQB1*; DRB1* allele's genetic variants between women with cervical precancerous lesions and healthy controls in Latvia.
Materials and methods: From January until April 2017 we enrolled 84 consecutive patients referred for colposcopy to Riga East University Hospital (Latvia) due to abnormal cervical cytology results. 57 women who came for a regular check-up and had normal cytology smears were included in the control group. Material from the cervix was taken for subsequent HLA genotyping of 13 DRB1*, 8 DQA1*, and 12 DQB1* alleles. Colposcopy was performed on all participants. In case of visual suspicion for CIN cervical biopsy was done.
Results: There were 57 "no CIN" patients, 23 histologically proven CIN 1 and 61 CIN2+ cases in the study population. CIN2+ was more often associated with DQA1*0401 (OR 6.68, 95% CI 1.47-30.29, p=0.014), DRB*15 (OR 2.99, 95% CI 1.22-7.39, p=0.017), DQB1*0401 (OR 2.91, 95%CI 1.11-7.68, p=0.03), DQA1*0103 (OR 2.72, 95% CI 1.02-7.21, p=0.045), DRB1*11 (OR 2.42, 95% CI 1.10-5.33, p=0.029) and DQB1*0301 (OR 1.94, 95% CI 1.12-3.38, p=0.018). Women with "no CIN" more often had DQB1*0501 (OR 0.17, 95% CI 0.04-0.81, p=0.026), DRB1*16 (OR 0.21, 95% CI 0.06-0.78, p=0.019), DQA1*0301 (OR 0.35, 95% CI 0.14-0.87, p=0.024) and DRB1*14 (OR 0.59, 95% CI 0.01-0.46, p=0.007).
Conclusions: In the current study we have demonstrated a strong association with risk and protective HLA class II alleles that are determined by the HLA-DRB1*; DQA1*; DQB1*.
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