在野外条件下测定母猪群中具有针对 PRRSV 的广泛交叉反应中和抗体的个体频率。

IF 3 2区 农林科学 Q1 VETERINARY SCIENCES
Ángeles Plaza-Soriano, Francisco Javier Martínez-Lobo, Laura Garza-Moreno, Jaime Castillo-Pérez, Elki Caballero, José María Castro, Isabel Simarro, Cinta Prieto
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引用次数: 0

摘要

背景:猪繁殖与呼吸综合征病毒(PRRSV)是一种重要的猪病原体,但有助于保护的免疫反应成分仍不完全清楚。广谱反应性中和抗体(bNAs)在预防异源病毒再感染方面可能起着至关重要的作用,尽管在现场和实验条件下它们的发生率都很低。本研究旨在评估现场条件下母猪出现 PRRSV bNA 的频率,并分析影响这些精英中和抗体出现的流行病学因素。研究人员从 11 个无关联猪场的繁殖母猪身上采集了血样,并按母猪的妊娠期对血样进行了分类。获得的血清用于针对六种 PRRSV 现场分离株和两种 MLV 株的病毒中和试验(VNs):结果:约有 7% 的母猪表现出了对检测组中所有病毒的中和活性,NA 的几何平均滴度 (GMT) 达到或超过 4 log2。如果将 PRRSV-2 分离物排除在外,精英中和者的比例将增加到 15%左右。对特定猪场的分析表明,NAs 滴度(GMT)和精英中和者比例均存在显著差异。PRRSV 不稳定的猪场和过去 12 个月内爆发过 PRRS 的猪场与近期未爆发过 PRRS 的稳定猪场相比,NAs 的 GMT 值更高。NAs的GMT随母猪的奇偶性逐渐增加,但幅度不大。母猪奇偶性对 bNA 反应的影响在稳定的猪场持续存在,但在不稳定或近期爆发疫情的猪场则不一定。最后,研究结果表明,接种疫苗的动物对猪场使用的疫苗病毒的 NA 滴度高于对野外病毒的 NA 滴度。然而,一部分母猪(约 15%)可被视为精英中和者,能有效识别各种 PRRSV 株系。重复暴露于 PRRSV 对激发这些 bNAs 起着至关重要的作用,在不稳定猪场和近期爆发过 PRRSV 的猪场观察到更高的频率。在稳定的猪场中,胎次对 bNA 滴度的影响微乎其微,这表明与 PRRSV 暴露史的影响相比,胎次的作用有限。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Determination of the frequency of individuals with broadly cross-reactive neutralizing antibodies against PRRSV in the sow population under field conditions.

Background: Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) is a significant swine pathogen, yet the immune response components contributing to protection remain incompletely understood. Broadly reactive neutralizing antibodies (bNAs) may play a crucial role in preventing reinfections by heterologous viruses, although their occurrence is considered low under both field and experimental conditions. This study aimed to assess the frequency of sows exhibiting bNAs against PRRSV under field conditions and to analyze the epidemiological factors influencing the occurrence of these elite neutralizers. Blood samples were collected from breeding sows across eleven unrelated pig farms, with samples categorized by parity. Serum obtained was utilized in virus neutralization assays (VNs) against six PRRSV field isolates and two MLV strains.

Results: Approximately 7% of the sows exhibited neutralization activity against all viruses in the panel, with a geometric mean of the titer (GMT) of NAs at or exceeding 4 log2. Exclusion of the PRRSV-2 isolate from the panel increased the proportion of elite neutralizers to around 15%. Farm-specific analysis revealed significant variations in both GMT of NAs and proportion of elite neutralizers. PRRSV unstable farms and those with a PRRS outbreak in the last 12 months displayed higher GMT of NAs compared to stable farms without recent outbreaks. The GMT of NAs showed a gradual, albeit moderate, increase with the parity of the sows. Parity's impact on bNA response was consistently observed in stable farms but not necessarily in unstable farms or those with recent outbreaks. Finally, the results indicated that vaccinated animals had higher NA titers against the vaccine virus used in the farm than against field viruses.

Conclusion: bNAs against heterologous isolates induced by PRRSV infection under field conditions are generally low, often falling below titers necessary for protection against reproductive failure. However, a subset of sows (approximately 15%) can be considered elite neutralizers, efficiently recognizing various PRRSV strains. Repeated exposures to PRRSV play a crucial role in eliciting these bNAs, with a higher frequency observed in unstable farms and those with recent outbreaks. In stable farms, parity only marginally influences bNA titers, highlighting its limited role compared to the impact of PRRSV exposure history.

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来源期刊
Porcine Health Management
Porcine Health Management Veterinary-Food Animals
CiteScore
5.40
自引率
5.90%
发文量
49
审稿时长
14 weeks
期刊介绍: Porcine Health Management (PHM) is an open access peer-reviewed journal that aims to publish relevant, novel and revised information regarding all aspects of swine health medicine and production.
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