Zahra Navi, Reza Falak, Mehdi Mohebali, Mohammad Bagher Molairad, Zabihollah Zarei, Mojgan Aryaeipour, Abbas Rahimi Foroushani, Mohammad Zibaei, Mohammad Bagher Rokni
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引用次数: 0
摘要
我们旨在开发一种间接酶联免疫吸附试验（ELISA），以评估弓形虫病患者血清中是否存在针对犬弓形虫和猫弓形虫体细胞抗原的特异性 IgG。通过接收者操作特征曲线（ROC）分析和使用似然比的 Youden's J 计算了间接-ELISA 的灵敏度、特异性、阳性预测值和阴性预测值。所有统计数据均使用 GraphPad Prism 8.4.3 版（Graph Pad Software，La Jolla，CA，USA）进行分析并绘制图表，并附有 95% 的置信区间 (CI)。犬白喉的敏感性、特异性、阳性预测值和阴性预测值分别为 100%、82%、79% 和 100%。上述变量对 T. cati 的预测值分别为 97%、82%、78% 和 98%。在这两个物种中，38、40、72、100 和 250 kDa 的五条免疫反应带很常见。弓形虫粗抗原在人类血清中的免疫原性很高。与 T. cati 体细胞抗原相比，T. canis 的免疫反应带要高出两倍。与两个物种同源的弓形虫排泄-分泌抗原不同，犬尾蚴和猫尾蚴的体细胞抗原在我们的 Western 印迹中显示出不同的免疫反应带。
Evaluation of Somatic Antigens of Adult Toxocara helminthes for Detection of Human Toxocariasis.
We aimed to develop an indirect enzyme-linked immunosorbent assay (ELISA) to evaluate the presence of specific IgG against Toxocara canis and Toxocara cati somatic antigens on the serum of patients with toxocariasis. The sensitivity, specificity, positive and negative predictive values for indirect-ELISA were calculated by receiver operating characteristic curve (ROC) analysis and Youden's J using Likelihood ratio. All statistics were analysed and graphs are plotted using GraphPad Prism version 8.4.3 (Graph Pad Software, La Jolla, CA, USA), with 95% confidence interval (CI). The sensitivity, specificity, positive and negative predictive values for T. canis were 100%, 82%, 79% and 100%, respectively. The mentioned variables for T. cati were 97%, 82%, 78% and 98%, respectively. Five immune reactive bands of 38, 40, 72, 100 and 250 kDa were common in both species. Toxocara crude antigens were highly immunogenic in human sera. Immunoreactive bands against T. canis compared to T. cati somatic antigen were about two times more. Unlike Toxocara excretory-secretory antigen, that was homologue in two species, somatic antigens of T. canis and T. cati showed different immunoreactive bands in our western blot.
期刊介绍：
Parasite Immunology is an international journal devoted to research on all aspects of parasite immunology in human and animal hosts. Emphasis has been placed on how hosts control parasites, and the immunopathological reactions which take place in the course of parasitic infections. The Journal welcomes original work on all parasites, particularly human parasitology, helminths, protozoa and ectoparasites.
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