AaBBX21-AaHY5 模块介导黄花蒿的光调青蒿素生物合成

IF 9.3 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Weizhi He, Hang Liu, Zhangkuanyu Wu, Qing Miao, Xinyi Hu, Xin Yan, Hangyu Wen, Yaojie Zhang, Xueqing Fu, Li Ren, Kexuan Tang, Ling Li
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引用次数: 0

摘要

倍半萜内酯青蒿素是一种重要的抗疟疾成分,由艾蒿(Artemisia annua L.)的腺体分泌毛状体产生。以前的研究表明,光能促进青蒿素的产生,但其潜在的调控机制仍然难以捉摸。在这项研究中,我们证明了光信号通路中的核心转录因子 ELONGATED HYPOCOTYL 5(HY5)不能单独促进青蒿素的生物合成,因为 AaHY5 与青蒿素生物合成基因启动子的结合不能激活这些基因的转录。转录组分析和酵母双杂交筛选发现,B-盒转录因子AaBBX21是AaHY5的潜在互作因子。AaBBX21 显示出一种三叶草特异性表达模式。此外,AaBBX21-AaHY5 复合物协同激活了下游基因 AaGSW1、AaMYB108 和 AaORA 启动子的转录,这些基因编码青蒿素生物合成的正调控因子。此外,AaHY5 和 AaBBX21 与青蒿 E3 泛素连接酶 CONSTITUTIVELY PHOTOMORPHOGENIC 1(COP1)发生了物理作用。在黑暗条件下,AaCOP1 会减少 AaHY5 和 AaBBX21 的积累,并抑制 AaHY5-AaBBX21 复合物下游基因的激活,从而解释了青蒿素在光照条件下产量增加的原因。我们的研究深入揭示了植物界中光对萜类化合物生物合成的核心调控机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

The AaBBX21–AaHY5 module mediates light-regulated artemisinin biosynthesis in Artemisia annua L.

The AaBBX21–AaHY5 module mediates light-regulated artemisinin biosynthesis in Artemisia annua L.

The sesquiterpene lactone artemisinin is an important anti-malarial component produced by the glandular secretory trichomes of sweet wormwood (Artemisia annua L.). Light was previously shown to promote artemisinin production, but the underlying regulatory mechanism remains elusive. In this study, we demonstrate that ELONGATED HYPOCOTYL 5 (HY5), a central transcription factor in the light signaling pathway, cannot promote artemisinin biosynthesis on its own, as the binding of AaHY5 to the promoters of artemisinin biosynthetic genes failed to activate their transcription. Transcriptome analysis and yeast two-hybrid screening revealed the B-box transcription factor AaBBX21 as a potential interactor with AaHY5. AaBBX21 showed a trichome-specific expression pattern. Additionally, the AaBBX21–AaHY5 complex cooperatively activated transcription from the promoters of the downstream genes AaGSW1, AaMYB108, and AaORA, encoding positive regulators of artemisinin biosynthesis. Moreover, AaHY5 and AaBBX21 physically interacted with the A. annua E3 ubiquitin ligase CONSTITUTIVELY PHOTOMORPHOGENIC 1 (COP1). In the dark, AaCOP1 decreased the accumulation of AaHY5 and AaBBX21 and repressed the activation of genes downstream of the AaHY5–AaBBX21 complex, explaining the enhanced production of artemisinin upon light exposure. Our study provides insights into the central regulatory mechanism by which light governs terpenoid biosynthesis in the plant kingdom.

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来源期刊
Journal of Integrative Plant Biology
Journal of Integrative Plant Biology 生物-生化与分子生物学
CiteScore
18.00
自引率
5.30%
发文量
220
审稿时长
3 months
期刊介绍: Journal of Integrative Plant Biology is a leading academic journal reporting on the latest discoveries in plant biology.Enjoy the latest news and developments in the field, understand new and improved methods and research tools, and explore basic biological questions through reproducible experimental design, using genetic, biochemical, cell and molecular biological methods, and statistical analyses.
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