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引用次数: 0
摘要
自 20 世纪 70 年代以来，透明质酸（HA）一直常用于治疗马和狗的骨关节炎和关节损伤。透明质酸是一种多糖，由 N-乙酰基-d-葡萄糖胺和 d-葡萄糖醛酸残基交替组成，聚合物分子的分子量高达 20 MDa。高分子量的 HA 溶于水后会形成粘性水凝胶，这使得 HA 溶液有别于大多数其他药物制剂。在马厩和狗舍检查中经常会遇到透明的粘性溶液，但由于缺乏分析方法，无法确定这些物质是否含有 HA 或其他未知化合物。本文介绍了一种简单的方法，可通过化学水解后的高碳层析和 MS/MS 分析来鉴定查获材料中的 HA。
Identification of hyaluronic acid in seized samples by Hypercarb chromatography-high resolution mass spectrometry.
Hyaluronic acid (HA) has been commonly used to treat osteoarthritis and joint injuries in horses and dogs since the 1970s. HA is a polysaccharide made up of alternating N-acetyl-d-glucosamine and d-glucuronic acid residues, with polymeric molecules achieving molecular weights as high as 20 MDa. High molecular weight HA forms a viscous hydrogel when dissolved in water, making HA solutions distinct from most other pharmaceutical preparations. Clear viscous solutions are often encountered during stable and kennel inspections, but in the absence of an analytical method, it is not possible to identify if these substances contain HA or other unknown compounds. This paper presents a simple method for the identification of HA in seized materials based on chemical hydrolysis followed by Hypercarb chromatography and MS/MS analysis.
期刊介绍：
As the incidence of drugs escalates in 21st century living, their detection and analysis have become increasingly important. Sport, the workplace, crime investigation, homeland security, the pharmaceutical industry and the environment are just some of the high profile arenas in which analytical testing has provided an important investigative tool for uncovering the presence of extraneous substances.
In addition to the usual publishing fare of primary research articles, case reports and letters, Drug Testing and Analysis offers a unique combination of; ‘How to’ material such as ‘Tutorials’ and ‘Reviews’, Speculative pieces (‘Commentaries’ and ‘Perspectives'', providing a broader scientific and social context to the aspects of analytical testing), ‘Annual banned substance reviews’ (delivering a critical evaluation of the methods used in the characterization of established and newly outlawed compounds).
Rather than focus on the application of a single technique, Drug Testing and Analysis employs a unique multidisciplinary approach to the field of controversial compound determination. Papers discussing chromatography, mass spectrometry, immunological approaches, 1D/2D gel electrophoresis, to name just a few select methods, are welcomed where their application is related to any of the six key topics listed below.
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