从土壤中分离的 Agromyces silvae sp.

IF 2.5 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Journal of microbiology and biotechnology Pub Date : 2024-07-28 Epub Date: 2024-05-23 DOI:10.4014/jmb.2404.04007
Hyosun Lee, Dhiraj Kumar Chaudhary, Dong-Uk Kim
{"title":"从土壤中分离的 Agromyces silvae sp.","authors":"Hyosun Lee, Dhiraj Kumar Chaudhary, Dong-Uk Kim","doi":"10.4014/jmb.2404.04007","DOIUrl":null,"url":null,"abstract":"<p><p>Three Gram-stain-positive, aerobic, rod-shaped, and non-motile bacteria, labelled as W11<sup>T</sup>, SW19<sup>T</sup>, and YR1<sup>T</sup>, were isolated from soil, and performed their polyphasic taxonomic investigation. The phylogenetic and 16S rRNA gene sequence analysis showed that strains W11<sup>T</sup>, SW19<sup>T</sup>, and YR1<sup>T</sup> belonged to the genera <i>Agromyces</i>, <i>Rathayibacter</i>, and <i>Nocardioides</i>, respectively. Strain W11<sup>T</sup> was closely affiliated with <i>Agromyces cavernae</i> SYSU K20354<sup>T</sup> (98.1%), strain SW19<sup>T</sup> showed the closest affiliation with <i>Rathayibacter rubneri</i> ZW T2_19<sup>T</sup> (97.0%), and strain YR1<sup>T</sup> was most closely related to <i>Nocardioides marmorisolisilvae</i> KIS18-7<sup>T</sup> (98.0%). The genome sizes of strains W11<sup>T</sup>, SW19<sup>T</sup>, and YR1<sup>T</sup> were 4,181,720 bp, 4,740,677 bp, and 4,228,226 bp, respectively, with DNA G+C contents of 70.5%, 64.2%, and 69.7%, respectively. Average nucleotide identity and digital DNA-DNA hybridization values of W11<sup>T</sup>, SW19<sup>T</sup>, and YR1<sup>T</sup> with their respective reference species were <79.6% and <23.6%, respectively. The predominant cellular fatty acids detected in strain W11<sup>T</sup> were anteiso-C<sub>15:0</sub>, iso-C<sub>16:0</sub>, and anteiso-C<sub>17:0</sub>. In strain SW19<sup>T</sup>, they were summed feature 9 (C<sub>16:0</sub> 10-methyl and/or iso-C<sub>17:1</sub>ω 9c), anteiso-C<sub>17:0</sub>, and anteiso-C<sub>15:0</sub>. Strain YR1<sup>T</sup> exhibited C<sub>18:1</sub>ω 9c, C<sub>18:0</sub> 10-methyl, TBSA, and anteiso-C<sub>15:0</sub> as its major cellular fatty acids. Overall, the polyphasic taxonomic comparisons indicated that strains W11<sup>T</sup>, SW19<sup>T</sup>, and YR1<sup>T</sup> represent novel species within the genera <i>Agromyces</i>, <i>Rathayibacter</i>, and <i>Nocardioides</i>, respectively. Accordingly, we propose the names <i>Agromyces silvae</i> sp. nov., with the type strain W11<sup>T</sup> (=KCTC 49818<sup>T</sup> =NBRC 115999<sup>T</sup>), <i>Rathayibacter soli</i> sp. nov., with the type strain SW19<sup>T</sup> (=KCTC 49860<sup>T</sup> =NBRC 116108<sup>T</sup>), and <i>Nocardioides terrisoli</i> sp. nov., with the type strain YR1<sup>T</sup> (=KCTC 49863<sup>T</sup> =NBRC 116165<sup>T</sup>).</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.5000,"publicationDate":"2024-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11294650/pdf/","citationCount":"0","resultStr":"{\"title\":\"<i>Agromyces silvae</i> sp. nov., <i>Rathayibacter soli</i> sp. nov., and <i>Nocardioides terrisoli</i> sp. nov., Isolated from Soil.\",\"authors\":\"Hyosun Lee, Dhiraj Kumar Chaudhary, Dong-Uk Kim\",\"doi\":\"10.4014/jmb.2404.04007\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Three Gram-stain-positive, aerobic, rod-shaped, and non-motile bacteria, labelled as W11<sup>T</sup>, SW19<sup>T</sup>, and YR1<sup>T</sup>, were isolated from soil, and performed their polyphasic taxonomic investigation. The phylogenetic and 16S rRNA gene sequence analysis showed that strains W11<sup>T</sup>, SW19<sup>T</sup>, and YR1<sup>T</sup> belonged to the genera <i>Agromyces</i>, <i>Rathayibacter</i>, and <i>Nocardioides</i>, respectively. Strain W11<sup>T</sup> was closely affiliated with <i>Agromyces cavernae</i> SYSU K20354<sup>T</sup> (98.1%), strain SW19<sup>T</sup> showed the closest affiliation with <i>Rathayibacter rubneri</i> ZW T2_19<sup>T</sup> (97.0%), and strain YR1<sup>T</sup> was most closely related to <i>Nocardioides marmorisolisilvae</i> KIS18-7<sup>T</sup> (98.0%). The genome sizes of strains W11<sup>T</sup>, SW19<sup>T</sup>, and YR1<sup>T</sup> were 4,181,720 bp, 4,740,677 bp, and 4,228,226 bp, respectively, with DNA G+C contents of 70.5%, 64.2%, and 69.7%, respectively. Average nucleotide identity and digital DNA-DNA hybridization values of W11<sup>T</sup>, SW19<sup>T</sup>, and YR1<sup>T</sup> with their respective reference species were <79.6% and <23.6%, respectively. The predominant cellular fatty acids detected in strain W11<sup>T</sup> were anteiso-C<sub>15:0</sub>, iso-C<sub>16:0</sub>, and anteiso-C<sub>17:0</sub>. In strain SW19<sup>T</sup>, they were summed feature 9 (C<sub>16:0</sub> 10-methyl and/or iso-C<sub>17:1</sub>ω 9c), anteiso-C<sub>17:0</sub>, and anteiso-C<sub>15:0</sub>. Strain YR1<sup>T</sup> exhibited C<sub>18:1</sub>ω 9c, C<sub>18:0</sub> 10-methyl, TBSA, and anteiso-C<sub>15:0</sub> as its major cellular fatty acids. Overall, the polyphasic taxonomic comparisons indicated that strains W11<sup>T</sup>, SW19<sup>T</sup>, and YR1<sup>T</sup> represent novel species within the genera <i>Agromyces</i>, <i>Rathayibacter</i>, and <i>Nocardioides</i>, respectively. Accordingly, we propose the names <i>Agromyces silvae</i> sp. nov., with the type strain W11<sup>T</sup> (=KCTC 49818<sup>T</sup> =NBRC 115999<sup>T</sup>), <i>Rathayibacter soli</i> sp. nov., with the type strain SW19<sup>T</sup> (=KCTC 49860<sup>T</sup> =NBRC 116108<sup>T</sup>), and <i>Nocardioides terrisoli</i> sp. nov., with the type strain YR1<sup>T</sup> (=KCTC 49863<sup>T</sup> =NBRC 116165<sup>T</sup>).</p>\",\"PeriodicalId\":16481,\"journal\":{\"name\":\"Journal of microbiology and biotechnology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2024-07-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11294650/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of microbiology and biotechnology\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.4014/jmb.2404.04007\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/5/23 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of microbiology and biotechnology","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.4014/jmb.2404.04007","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/5/23 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

从土壤中分离出三株革兰氏染色阳性、需氧、杆状、非运动性细菌,分别命名为 W11T、SW19T 和 YR1T,并进行了多相分类研究。系统进化和 16S rRNA 基因序列分析表明,菌株 W11T、SW19T 和 YR1T 分别属于 Agromyces 属、Rathayibacter 属和 Nocardioides 属。菌株 W11T 与 Agromyces cavernae SYSU K20354T 关系密切(98.1%),菌株 SW19T 与 Rathayibacter rubneri ZW T2_19T 关系最密切(97.0%),而菌株 YR1T 与 Nocardioides marmorisolisilvae KIS18-7T 关系最密切(98.0%)。菌株 W11T、SW19T 和 YR1T 的基因组大小分别为 4,181,720 bp、4,740,677 bp 和 4,228,226 bp,DNA G+C 含量分别为 70.5%、64.2% 和 69.7%。W11T、SW19T 和 YR1T 与各自参考物种的平均核苷酸相同度和数字 DNA-DNA 杂交值分别为 5:0、isoC16:0 和 anteiso-C17:0。在菌株 SW19T 中,它们的总特征值为 9(C16:0 10-甲基和/或异 C17:1ω 9c)、前异 C17:0 和前异 C15:0。菌株 YR1T 的主要细胞脂肪酸为 C18:1ω9c、C18:0 10-甲基、TBSA 和前异构-C15:0。总之,多相分类比较表明,W11T、SW19T 和 YR1T 菌株分别代表 Agromyces 属、Rathayibacter 属和 Nocardioides 属中的新物种。因此,我们建议将模式菌株 W11T(=KCTC 49818T =NBRC 115999T)命名为 Agromyces silvae sp.nov.;将模式菌株 SW19T(=KCTC 49860T =NBRC 116108T)命名为 Rathayibacter soli sp.nov.;将模式菌株 YR1T(=KCTC 49863T =NBRC 116165T)命名为 Nocardioides terrisoli sp.nov.。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Agromyces silvae sp. nov., Rathayibacter soli sp. nov., and Nocardioides terrisoli sp. nov., Isolated from Soil.

Three Gram-stain-positive, aerobic, rod-shaped, and non-motile bacteria, labelled as W11T, SW19T, and YR1T, were isolated from soil, and performed their polyphasic taxonomic investigation. The phylogenetic and 16S rRNA gene sequence analysis showed that strains W11T, SW19T, and YR1T belonged to the genera Agromyces, Rathayibacter, and Nocardioides, respectively. Strain W11T was closely affiliated with Agromyces cavernae SYSU K20354T (98.1%), strain SW19T showed the closest affiliation with Rathayibacter rubneri ZW T2_19T (97.0%), and strain YR1T was most closely related to Nocardioides marmorisolisilvae KIS18-7T (98.0%). The genome sizes of strains W11T, SW19T, and YR1T were 4,181,720 bp, 4,740,677 bp, and 4,228,226 bp, respectively, with DNA G+C contents of 70.5%, 64.2%, and 69.7%, respectively. Average nucleotide identity and digital DNA-DNA hybridization values of W11T, SW19T, and YR1T with their respective reference species were <79.6% and <23.6%, respectively. The predominant cellular fatty acids detected in strain W11T were anteiso-C15:0, iso-C16:0, and anteiso-C17:0. In strain SW19T, they were summed feature 9 (C16:0 10-methyl and/or iso-C17:1ω 9c), anteiso-C17:0, and anteiso-C15:0. Strain YR1T exhibited C18:1ω 9c, C18:0 10-methyl, TBSA, and anteiso-C15:0 as its major cellular fatty acids. Overall, the polyphasic taxonomic comparisons indicated that strains W11T, SW19T, and YR1T represent novel species within the genera Agromyces, Rathayibacter, and Nocardioides, respectively. Accordingly, we propose the names Agromyces silvae sp. nov., with the type strain W11T (=KCTC 49818T =NBRC 115999T), Rathayibacter soli sp. nov., with the type strain SW19T (=KCTC 49860T =NBRC 116108T), and Nocardioides terrisoli sp. nov., with the type strain YR1T (=KCTC 49863T =NBRC 116165T).

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Journal of microbiology and biotechnology
Journal of microbiology and biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-MICROBIOLOGY
CiteScore
5.50
自引率
3.60%
发文量
151
审稿时长
2 months
期刊介绍: The Journal of Microbiology and Biotechnology (JMB) is a monthly international journal devoted to the advancement and dissemination of scientific knowledge pertaining to microbiology, biotechnology, and related academic disciplines. It covers various scientific and technological aspects of Molecular and Cellular Microbiology, Environmental Microbiology and Biotechnology, Food Biotechnology, and Biotechnology and Bioengineering (subcategories are listed below). Launched in March 1991, the JMB is published by the Korean Society for Microbiology and Biotechnology (KMB) and distributed worldwide.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信