Jiangbo Guo, Hanyang Liu, Yang Xu, Lu Li, Cuihua Xin
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引用次数: 0
摘要
囊泡螯合是提高植物对镉(Cd)和砷(As)耐受性的一种潜在策略。本研究发现,在拟南芥中异位过表达酵母衍生的 ScSMF2 可增强转基因植物对镉和砷的积累和耐受性。SCSMF2定位于液泡膜上,在植物细胞中经农渗瞬时表达后形成点状结构。转基因拟南芥在含 Cd 或 As 的培养基上培养时,根系伸长和芽重的延迟较小,而 Cd、As(III)和 As(V)的积累较多。过量表达 ScSMF2 会促进转基因拟南芥中镉和砷的积累,在土壤中培养时,镉和砷的积累量是 WT 植物的两倍多。这项研究深入揭示了重金属在植物体内的囊泡螯合机制,并为提高植物对重金属的植物修复能力提供了一种潜在的策略。
Ectopic expression of the yeast Mn2+ transporter SMF2 enhances tolerance and resistance to cadmium and arsenic in transgenic Arabidopsis.
Vesicular sequestration is a potential strategy for enhancing plant tolerance to cadmium (Cd) and arsenic (As). In this study, the ectopic overexpression of yeast-derived ScSMF2 in Arabidopsis thaliana was found to enhance the accumulation and tolerance of Cd and As in transgenic plants. ScSMF2 was localized on vacuole membranes and formed puncta structures in plant cells when agro-infiltrated for transient expression. Transgenic Arabidopsis showed less retardation on root elongation and shoot weight and more accumulation of Cd, As (III) and As (V) when cultured on medium containing Cd or As. Overexpression of ScSMF2 promoted accumulation of Cd and arsenic in transgenic Arabidopsis, which were over twice higher than in WT plants when cultured in soil. This study provides insights into the mechanisms involved in the vesicular sequestration of heavy metals in plant and presents a potential strategy for enhancing the phytoremediation capacity of plants toward heavy metals.
期刊介绍:
The International Journal of Phytoremediation (IJP) is the first journal devoted to the publication of laboratory and field research describing the use of plant systems to solve environmental problems by enabling the remediation of soil, water, and air quality and by restoring ecosystem services in managed landscapes. Traditional phytoremediation has largely focused on soil and groundwater clean-up of hazardous contaminants. Phytotechnology expands this umbrella to include many of the natural resource management challenges we face in cities, on farms, and other landscapes more integrated with daily public activities. Wetlands that treat wastewater, rain gardens that treat stormwater, poplar tree plantings that contain pollutants, urban tree canopies that treat air pollution, and specialized plants that treat decommissioned mine sites are just a few examples of phytotechnologies.