Chun Xie, Kuiting Chen, Zhekun Chen, Yingxin Hu, Linqiang Pan
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引用次数: 0
摘要
DNA 纳米结构已被用于研究生物机械过程和构建人工纳米系统。许多应用场景都要求纳米装置能够稳健地产生较大的单分子力。然而,现有的大多数动态 DNA 纳米结构都是由空间上分离的 DNA 链之间的概率杂交反应触发的,只能非确定地产生相对较小的压缩力(≈0.4 皮牛顿 (pN))。在这里,我们开发了一种由插层剂触发的动态 DNA 折纸纳米结构,插层剂与纳米结构之间的大量局部结合反应可共同产生相对较大的压缩力(≈11.2 皮牛顿)。具有不同刚度的生物分子负载、3、4 和 6 螺旋 DNA 束都能在压缩力的作用下有效弯曲。这项工作为在合成纳米系统中构建高度化学机械耦合的分子机器提供了一种稳健而强大的力生成工具。
A Chemo-Mechanically Coupled DNA Origami Clamp Capable of Generating Robust Compression Forces.
DNA nanostructures have been utilized to study biological mechanical processes and construct artificial nanosystems. Many application scenarios necessitate nanodevices able to robustly generate large single molecular forces. However, most existing dynamic DNA nanostructures are triggered by probabilistic hybridization reactions between spatially separated DNA strands, which only non-deterministically generate relatively small compression forces (≈0.4 piconewtons (pN)). Here, an intercalator-triggered dynamic DNA origami nanostructure is developed, where large amounts of local binding reactions between intercalators and the nanostructure collectively lead to the robust generation of relatively large compression forces (≈11.2 pN). Biomolecular loads with different stiffnesses, 3, 4, and 6-helix DNA bundles are efficiently bent by the compression forces. This work provides a robust and powerful force-generation tool for building highly chemo-mechanically coupled molecular machines in synthetic nanosystems.
期刊介绍:
Small serves as an exceptional platform for both experimental and theoretical studies in fundamental and applied interdisciplinary research at the nano- and microscale. The journal offers a compelling mix of peer-reviewed Research Articles, Reviews, Perspectives, and Comments.
With a remarkable 2022 Journal Impact Factor of 13.3 (Journal Citation Reports from Clarivate Analytics, 2023), Small remains among the top multidisciplinary journals, covering a wide range of topics at the interface of materials science, chemistry, physics, engineering, medicine, and biology.
Small's readership includes biochemists, biologists, biomedical scientists, chemists, engineers, information technologists, materials scientists, physicists, and theoreticians alike.