Wissam Reti, Warda Djoudi, Ibtissem Djinni, Hanane Belabbas, Sara Haddad, Amel Hamma, Samiha Souagui, Mouloud Kecha
{"title":"利用响应面方法优化从公共垃圾堆中提取的潮湿链霉菌 DBPb2 对铅的生物吸附率","authors":"Wissam Reti, Warda Djoudi, Ibtissem Djinni, Hanane Belabbas, Sara Haddad, Amel Hamma, Samiha Souagui, Mouloud Kecha","doi":"10.1007/s11270-024-07293-0","DOIUrl":null,"url":null,"abstract":"<p>Pollution by heavy metals is one of the risks threating the human health and the environment, and reducing the concentrations of these pollutants constitutes a major challenge, in particular by using alternative bioremediation techniques. Actinobacteria are frequently proposed as an environmental cleaner of several emerging pollutants such as heavy metals. In this study, 24 actinobacteria resistant strains to heavy metals isolated from the Boulimat public landfill in Bejaia, Algeria, were evaluated for their heavy metal removal potential. Two different screenings were conducted, determination of minimum inhibitory concentration of the isolates to metal ions and the percentages of metals biosorption in batch experiment using the Atomic Absorption Spectroscopy. <i>Streptomyces humidus</i> DBPb2 strain, was selected as the most efficient isolate, identified based on morphological, physiological and 16S rRNA gene sequencing, by a highest minimum inhibitory concentrations to various metals mainly lead, with a MIC reaching 4000 mg.L⁻<sup>1</sup> and has proven its efficiency to reduce the concentration of Pb, Fe and Cu in batch experiments with 66.47%, 33.16% and 27.39% respectively. Rotatable Central Composite Design was used to optimize the lead bisorption yield studying the influence of four operating parameters: pH, stirring speed, incubation time, and inoculum size. The optimal conditions were found for pH= 7, at stirring speed of 84 rpm under incubation time of 3 days, and 3 agar cylinders as inoculum reaching a lead biosorption yield of 100%, for an initial concentration of 100 mg.L<sup>-1</sup>. The metal-resistance mechanisms were identified in DBPb2 strain, the production of siderophores and cell wall bioaccumulation by the interaction of different functional groups (carboxylic, hydroxyl and amine groups) detected by infrared spectroscopy analysis. Therefore, this present study confirms the possibility of exploiting the DBPb2 strain in the bioremediation of lead from polluted environments such as soils and wastewater.</p>","PeriodicalId":808,"journal":{"name":"Water, Air, & Soil Pollution","volume":null,"pages":null},"PeriodicalIF":3.8000,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Optimization of Lead Biosorption Yield by Streptomyces humidus DBPb2 Derived from a Public Waste Dump Using the Response Surface Methodology\",\"authors\":\"Wissam Reti, Warda Djoudi, Ibtissem Djinni, Hanane Belabbas, Sara Haddad, Amel Hamma, Samiha Souagui, Mouloud Kecha\",\"doi\":\"10.1007/s11270-024-07293-0\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Pollution by heavy metals is one of the risks threating the human health and the environment, and reducing the concentrations of these pollutants constitutes a major challenge, in particular by using alternative bioremediation techniques. Actinobacteria are frequently proposed as an environmental cleaner of several emerging pollutants such as heavy metals. In this study, 24 actinobacteria resistant strains to heavy metals isolated from the Boulimat public landfill in Bejaia, Algeria, were evaluated for their heavy metal removal potential. Two different screenings were conducted, determination of minimum inhibitory concentration of the isolates to metal ions and the percentages of metals biosorption in batch experiment using the Atomic Absorption Spectroscopy. <i>Streptomyces humidus</i> DBPb2 strain, was selected as the most efficient isolate, identified based on morphological, physiological and 16S rRNA gene sequencing, by a highest minimum inhibitory concentrations to various metals mainly lead, with a MIC reaching 4000 mg.L⁻<sup>1</sup> and has proven its efficiency to reduce the concentration of Pb, Fe and Cu in batch experiments with 66.47%, 33.16% and 27.39% respectively. 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Optimization of Lead Biosorption Yield by Streptomyces humidus DBPb2 Derived from a Public Waste Dump Using the Response Surface Methodology
Pollution by heavy metals is one of the risks threating the human health and the environment, and reducing the concentrations of these pollutants constitutes a major challenge, in particular by using alternative bioremediation techniques. Actinobacteria are frequently proposed as an environmental cleaner of several emerging pollutants such as heavy metals. In this study, 24 actinobacteria resistant strains to heavy metals isolated from the Boulimat public landfill in Bejaia, Algeria, were evaluated for their heavy metal removal potential. Two different screenings were conducted, determination of minimum inhibitory concentration of the isolates to metal ions and the percentages of metals biosorption in batch experiment using the Atomic Absorption Spectroscopy. Streptomyces humidus DBPb2 strain, was selected as the most efficient isolate, identified based on morphological, physiological and 16S rRNA gene sequencing, by a highest minimum inhibitory concentrations to various metals mainly lead, with a MIC reaching 4000 mg.L⁻1 and has proven its efficiency to reduce the concentration of Pb, Fe and Cu in batch experiments with 66.47%, 33.16% and 27.39% respectively. Rotatable Central Composite Design was used to optimize the lead bisorption yield studying the influence of four operating parameters: pH, stirring speed, incubation time, and inoculum size. The optimal conditions were found for pH= 7, at stirring speed of 84 rpm under incubation time of 3 days, and 3 agar cylinders as inoculum reaching a lead biosorption yield of 100%, for an initial concentration of 100 mg.L-1. The metal-resistance mechanisms were identified in DBPb2 strain, the production of siderophores and cell wall bioaccumulation by the interaction of different functional groups (carboxylic, hydroxyl and amine groups) detected by infrared spectroscopy analysis. Therefore, this present study confirms the possibility of exploiting the DBPb2 strain in the bioremediation of lead from polluted environments such as soils and wastewater.
期刊介绍:
Water, Air, & Soil Pollution is an international, interdisciplinary journal on all aspects of pollution and solutions to pollution in the biosphere. This includes chemical, physical and biological processes affecting flora, fauna, water, air and soil in relation to environmental pollution. Because of its scope, the subject areas are diverse and include all aspects of pollution sources, transport, deposition, accumulation, acid precipitation, atmospheric pollution, metals, aquatic pollution including marine pollution and ground water, waste water, pesticides, soil pollution, sewage, sediment pollution, forestry pollution, effects of pollutants on humans, vegetation, fish, aquatic species, micro-organisms, and animals, environmental and molecular toxicology applied to pollution research, biosensors, global and climate change, ecological implications of pollution and pollution models. Water, Air, & Soil Pollution also publishes manuscripts on novel methods used in the study of environmental pollutants, environmental toxicology, environmental biology, novel environmental engineering related to pollution, biodiversity as influenced by pollution, novel environmental biotechnology as applied to pollution (e.g. bioremediation), environmental modelling and biorestoration of polluted environments.
Articles should not be submitted that are of local interest only and do not advance international knowledge in environmental pollution and solutions to pollution. Articles that simply replicate known knowledge or techniques while researching a local pollution problem will normally be rejected without review. Submitted articles must have up-to-date references, employ the correct experimental replication and statistical analysis, where needed and contain a significant contribution to new knowledge. The publishing and editorial team sincerely appreciate your cooperation.
Water, Air, & Soil Pollution publishes research papers; review articles; mini-reviews; and book reviews.