用于等温扩增目的的 Bst 聚合酶蛋白质工程

IF 0.7 Q4 CHEMISTRY, MULTIDISCIPLINARY

Moscow University Chemistry Bulletin Pub Date : 2024-06-04 DOI:10.3103/S002713142470007X

A. S. Cherkashina, O. O. Mikheeva, V. G. Akimkin

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引用次数: 0

摘要

摘要本文综述了利用各种方法对 Bst 聚合酶进行蛋白质工程改造的情况。为了改造酶，采用了生产嵌合蛋白、定向进化、定向和随机诱变等方法。文中介绍了成功改变酶特性的例子，如催化活性、加工性、热稳定性和对抑制剂的抗性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Protein Engineering of Bst Polymerase for Isothermal Amplification Purposes

This paper reviews the protein engineering of Bst polymerase using various methods. To modify the enzyme, approaches such as the production of chimeric proteins, directed evolution, and directed and random mutagenesis are used. Examples of successful changes in enzyme properties such as catalytic activity, processivity, thermal stability, and resistance to inhibitors are described.

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来源期刊

Moscow University Chemistry Bulletin CHEMISTRY, MULTIDISCIPLINARY-

CiteScore

1.30

自引率

14.30%

发文量

期刊介绍： Moscow University Chemistry Bulletin is a journal that publishes review articles, original research articles, and short communications on various areas of basic and applied research in chemistry, including medical chemistry and pharmacology.