Shazia Arif, Hafiz Muhammad Ahmed, Safira Attacha, Kotb A. Attia, Arif Ahmed Mohammed, Saira Ishaq, Shahid Iqbal Awan, Sajid Fiaz, Waseem Alam, Seung Hwan Yang
{"title":"沙棘（Hippophae rhamnoides L.）中合成类胡萝卜素的 Hr-Lcyb 和 Hr-Psy 基因的分子特征和转录组比较分析","authors":"Shazia Arif, Hafiz Muhammad Ahmed, Safira Attacha, Kotb A. Attia, Arif Ahmed Mohammed, Saira Ishaq, Shahid Iqbal Awan, Sajid Fiaz, Waseem Alam, Seung Hwan Yang","doi":"10.1007/s42729-024-01891-x","DOIUrl":null,"url":null,"abstract":"<p>Sea buckthorn (<i>Hippophae rhamnoides</i>) is an important medicinal plant of Southeast-Asia, enriched with various antioxidant components like β-carotene, alpha-tocopherol, catechin and folacin. Carotenoids are the bioactive substances abundantly present in sea buckthorn berries providing it an enticing yellowish, or orange red color. We performed the phylogenetic analysis, and conserved domain analysis to observe the evolutionary history and functional cites of carotenoids biosynthesis genes respectively. Further relative expression of two carotenoids biosynthesis genes <i>Lycopene β-cyclase (Hr-Lcyb)</i> and <i>phytoene synthase</i> (<i>Hr-Psy</i>) in was conducted in various tissues of sea buckthorn plants. Our results revealed that both the carotenoid synthesis genes <i>Hr-Lcyb</i> and <i>Hr-Psy</i> showed homology with already reported <i>Arabidopsis thaliana</i> genes (<i>AT3G10230</i> and <i>AT5G17230</i>). Relative expression analysis indicated that expression of <i>Hr-Lcyb</i> and <i>Hr-Psy</i> genes in leaves and floral organs of sea buckthorn was one-fold higher than other tissues. This study will open the door for plant biologists for further characterization of carotenoids synthesis genes and their regulatory processes in specific tissues of carotenoid rich fruit plants.</p>","PeriodicalId":17042,"journal":{"name":"Journal of Soil Science and Plant Nutrition","volume":"40 1","pages":""},"PeriodicalIF":3.4000,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Molecular Characterization and Comparative Transcriptomic Analysis of Hr-Lcyb and Hr-Psy Genes for Carotenoids Synthesis in Sea Buckthorn (Hippophae rhamnoides L.)\",\"authors\":\"Shazia Arif, Hafiz Muhammad Ahmed, Safira Attacha, Kotb A. Attia, Arif Ahmed Mohammed, Saira Ishaq, Shahid Iqbal Awan, Sajid Fiaz, Waseem Alam, Seung Hwan Yang\",\"doi\":\"10.1007/s42729-024-01891-x\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Sea buckthorn (<i>Hippophae rhamnoides</i>) is an important medicinal plant of Southeast-Asia, enriched with various antioxidant components like β-carotene, alpha-tocopherol, catechin and folacin. Carotenoids are the bioactive substances abundantly present in sea buckthorn berries providing it an enticing yellowish, or orange red color. We performed the phylogenetic analysis, and conserved domain analysis to observe the evolutionary history and functional cites of carotenoids biosynthesis genes respectively. Further relative expression of two carotenoids biosynthesis genes <i>Lycopene β-cyclase (Hr-Lcyb)</i> and <i>phytoene synthase</i> (<i>Hr-Psy</i>) in was conducted in various tissues of sea buckthorn plants. Our results revealed that both the carotenoid synthesis genes <i>Hr-Lcyb</i> and <i>Hr-Psy</i> showed homology with already reported <i>Arabidopsis thaliana</i> genes (<i>AT3G10230</i> and <i>AT5G17230</i>). Relative expression analysis indicated that expression of <i>Hr-Lcyb</i> and <i>Hr-Psy</i> genes in leaves and floral organs of sea buckthorn was one-fold higher than other tissues. This study will open the door for plant biologists for further characterization of carotenoids synthesis genes and their regulatory processes in specific tissues of carotenoid rich fruit plants.</p>\",\"PeriodicalId\":17042,\"journal\":{\"name\":\"Journal of Soil Science and Plant Nutrition\",\"volume\":\"40 1\",\"pages\":\"\"},\"PeriodicalIF\":3.4000,\"publicationDate\":\"2024-07-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Soil Science and Plant Nutrition\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1007/s42729-024-01891-x\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"ENVIRONMENTAL SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Soil Science and Plant Nutrition","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s42729-024-01891-x","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
Molecular Characterization and Comparative Transcriptomic Analysis of Hr-Lcyb and Hr-Psy Genes for Carotenoids Synthesis in Sea Buckthorn (Hippophae rhamnoides L.)
Sea buckthorn (Hippophae rhamnoides) is an important medicinal plant of Southeast-Asia, enriched with various antioxidant components like β-carotene, alpha-tocopherol, catechin and folacin. Carotenoids are the bioactive substances abundantly present in sea buckthorn berries providing it an enticing yellowish, or orange red color. We performed the phylogenetic analysis, and conserved domain analysis to observe the evolutionary history and functional cites of carotenoids biosynthesis genes respectively. Further relative expression of two carotenoids biosynthesis genes Lycopene β-cyclase (Hr-Lcyb) and phytoene synthase (Hr-Psy) in was conducted in various tissues of sea buckthorn plants. Our results revealed that both the carotenoid synthesis genes Hr-Lcyb and Hr-Psy showed homology with already reported Arabidopsis thaliana genes (AT3G10230 and AT5G17230). Relative expression analysis indicated that expression of Hr-Lcyb and Hr-Psy genes in leaves and floral organs of sea buckthorn was one-fold higher than other tissues. This study will open the door for plant biologists for further characterization of carotenoids synthesis genes and their regulatory processes in specific tissues of carotenoid rich fruit plants.
期刊介绍：
The Journal of Soil Science and Plant Nutrition is an international, peer reviewed journal devoted to publishing original research findings in the areas of soil science, plant nutrition, agriculture and environmental science.
Soil sciences submissions may cover physics, chemistry, biology, microbiology, mineralogy, ecology, pedology, soil classification and amelioration.
Plant nutrition and agriculture submissions may include plant production, physiology and metabolism of plants, plant ecology, diversity and sustainability of agricultural systems, organic and inorganic fertilization in relation to their impact on yields, quality of plants and ecological systems, and agroecosystems studies.
Submissions covering soil degradation, environmental pollution, nature conservation, and environmental protection are also welcome.
The journal considers for publication original research articles, technical notes, short communication, and reviews (both voluntary and by invitation), and letters to the editor.
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