吲哚三碳菁染料敏化微生物细胞的光损伤机制

IF 0.8 4区化学 Q4 SPECTROSCOPY

Journal of Applied Spectroscopy Pub Date : 2024-07-05 DOI:10.1007/s10812-024-01759-z

A. A. Tabolich, A. I. Tretyakova, L. G. Plavskaya, T. S. Ananich, R. K. Nahorny, V. Y. Plavskii, L. S. Lyashenko, E. S. Voropay, A. P. Lugovski

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引用次数: 0

摘要

研究了吲哚三羰花青染料 PK220 致敏的大肠杆菌革兰氏阴性菌细胞暴露于 LED 光源辐射时的光损伤机制，LED 光源的发射光谱最大波长 λmax = 745 nm，与该染料的长波长吸收带相对应。细胞自身（未被活化剂添加剂增强）光诱导化学发光的强度被用来评估光生物效应的大小。细胞悬浮液辐照停止后立即产生的光破坏主要来自单线态氧，其次是 H2O2。羟基自由基在敏化光化学过程的机制中没有发挥重要作用。

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Mechanism of Photodamage of Microbial Cells Sensitized by Indotricarbocyanine Dye

The mechanism of photodamage of E. coli Gram-negative bacterial cells sensitized with indotricarbocyanine dye PK220 was studied upon exposure to radiation from an LED source with emission spectrum maximum wavelength λ_max = 745 nm corresponding to the long-wavelength absorption band of the dye. The intensity of the cells' own (not enhanced by activator additives) light-induced chemiluminescence was used to assess the magnitude of the photobiological effect. The main contribution to their photodestruction immediately after cessation of cell-suspension irradiation came from singlet oxygen and to a lesser extent from H₂O₂. Hydroxyl radicals did not play a significant role in the mechanism of sensitized photochemical processes.

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来源期刊

Journal of Applied Spectroscopy SPECTROSCOPY-

CiteScore

1.30

自引率

14.30%

发文量

145

审稿时长

2.5 months

期刊介绍： Journal of Applied Spectroscopy reports on many key applications of spectroscopy in chemistry, physics, metallurgy, and biology. An increasing number of papers focus on the theory of lasers, as well as the tremendous potential for the practical applications of lasers in numerous fields and industries.