日本北部健康人口腔和皮肤表面葡萄球菌分离物的流行率、遗传特征和抗菌药耐药性。

IF 4.7 3区医学 Q1 INFECTIOUS DISEASES

Journal of Infection and Public Health Pub Date : 2024-07-02 DOI:10.1016/j.jiph.2024.102488

Mina Hirose , Meiji Soe Aung , Yusuke Fujita , Sayaka Sakakibara , Erika Minowa-Suzuki , Maiko Otomo , Yoshihito Kurashige , Masato Saitoh , Yukito Hirose , Nobumichi Kobayashi

{"title":"日本北部健康人口腔和皮肤表面葡萄球菌分离物的流行率、遗传特征和抗菌药耐药性。","authors":"Mina Hirose , Meiji Soe Aung , Yusuke Fujita , Sayaka Sakakibara , Erika Minowa-Suzuki , Maiko Otomo , Yoshihito Kurashige , Masato Saitoh , Yukito Hirose , Nobumichi Kobayashi","doi":"10.1016/j.jiph.2024.102488","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>Oral cavity is an ecological niche for colonization of staphylococci, which are a major bacterial species causing community-acquired infections in humans. In this study, prevalence, and characteristics of staphylococci in oral cavity and skin of healthy individuals were investigated in northern Japan.</p></div><div><h3>Methods</h3><p>Saliva from oral cavity and swab from skin surface of hand were collected and cultured on selective media. Species of the isolates were identified genetically, and ST was determined for <em>S. aureus</em> and <em>S. argenteus</em>. Genes associated with antimicrobial resistance were detected by PCR.</p></div><div><h3>Results</h3><p>Among 166 participants, a total of 75 <em>S. aureus</em> isolates were obtained from 61 individuals (37 %), and recovered more frequently in oral cavity (n = 48) than skin (n = 27). Among 23 STs identified in <em>S. aureus</em> isolates, ST8 (CC8), ST15 (CC15), and ST188 (CC1) were the most common (10 isolates each), with STs of CC1 being dominant (17 isolates). Methicillin-resistant <em>S. aureus</em> (MRSA) was isolated in the skin of two individuals and belonged to ST1 and ST6. Resistance to erythromycin and gentamicin associated with <em>erm(A)</em> and <em>aac(6’)-Ie-aph(2”)-Ia</em>, respectively, was more commonly found in ST5 and ST8 isolates. One <em>S. argenteus</em> isolate (ST2250, <em>mecA</em>-negative) was recovered from oral cavity of a participant (0.6 %). A total of 186 isolates of coagulase-negative staphylococci (CoNS) were recovered from 102 participants and identified into 14 species, with <em>S. warneri</em> being the most common (n = 52), followed by <em>S. capitis</em> (n = 42), <em>S. saprophyticus</em> (n = 20) and <em>S. haemolyticus</em> (n = 19). <em>mecA</em> was detected in <em>S. saprophyticus</em>, <em>S. haemolyticus</em>, and <em>S. caprae</em>, while arginine-catabolic mobile element (ACME) in only <em>S. capitis</em> and <em>S. epidermidis</em>.</p></div><div><h3>Conclusion</h3><p><em>S. aureus</em> was more prevalent in oral cavity than skin surface, belonging to three major STs, with CC1 being a dominant lineage. The prevalence of antimicrobial resistance was distinct depending on CoNS species.</p></div>","PeriodicalId":16087,"journal":{"name":"Journal of Infection and Public Health","volume":null,"pages":null},"PeriodicalIF":4.7000,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1876034124002223/pdfft?md5=c42c6948699c14c1cf765eeb776b4158&pid=1-s2.0-S1876034124002223-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Prevalence, genetic characteristics, and antimicrobial resistance of staphylococcal isolates from oral cavity and skin surface of healthy individuals in northern Japan\",\"authors\":\"Mina Hirose , Meiji Soe Aung , Yusuke Fujita , Sayaka Sakakibara , Erika Minowa-Suzuki , Maiko Otomo , Yoshihito Kurashige , Masato Saitoh , Yukito Hirose , Nobumichi Kobayashi\",\"doi\":\"10.1016/j.jiph.2024.102488\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>Oral cavity is an ecological niche for colonization of staphylococci, which are a major bacterial species causing community-acquired infections in humans. In this study, prevalence, and characteristics of staphylococci in oral cavity and skin of healthy individuals were investigated in northern Japan.</p></div><div><h3>Methods</h3><p>Saliva from oral cavity and swab from skin surface of hand were collected and cultured on selective media. Species of the isolates were identified genetically, and ST was determined for <em>S. aureus</em> and <em>S. argenteus</em>. Genes associated with antimicrobial resistance were detected by PCR.</p></div><div><h3>Results</h3><p>Among 166 participants, a total of 75 <em>S. aureus</em> isolates were obtained from 61 individuals (37 %), and recovered more frequently in oral cavity (n = 48) than skin (n = 27). Among 23 STs identified in <em>S. aureus</em> isolates, ST8 (CC8), ST15 (CC15), and ST188 (CC1) were the most common (10 isolates each), with STs of CC1 being dominant (17 isolates). Methicillin-resistant <em>S. aureus</em> (MRSA) was isolated in the skin of two individuals and belonged to ST1 and ST6. Resistance to erythromycin and gentamicin associated with <em>erm(A)</em> and <em>aac(6’)-Ie-aph(2”)-Ia</em>, respectively, was more commonly found in ST5 and ST8 isolates. One <em>S. argenteus</em> isolate (ST2250, <em>mecA</em>-negative) was recovered from oral cavity of a participant (0.6 %). A total of 186 isolates of coagulase-negative staphylococci (CoNS) were recovered from 102 participants and identified into 14 species, with <em>S. warneri</em> being the most common (n = 52), followed by <em>S. capitis</em> (n = 42), <em>S. saprophyticus</em> (n = 20) and <em>S. haemolyticus</em> (n = 19). <em>mecA</em> was detected in <em>S. saprophyticus</em>, <em>S. haemolyticus</em>, and <em>S. caprae</em>, while arginine-catabolic mobile element (ACME) in only <em>S. capitis</em> and <em>S. epidermidis</em>.</p></div><div><h3>Conclusion</h3><p><em>S. aureus</em> was more prevalent in oral cavity than skin surface, belonging to three major STs, with CC1 being a dominant lineage. The prevalence of antimicrobial resistance was distinct depending on CoNS species.</p></div>\",\"PeriodicalId\":16087,\"journal\":{\"name\":\"Journal of Infection and Public Health\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.7000,\"publicationDate\":\"2024-07-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S1876034124002223/pdfft?md5=c42c6948699c14c1cf765eeb776b4158&pid=1-s2.0-S1876034124002223-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Infection and Public Health\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1876034124002223\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Infection and Public Health","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1876034124002223","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}

引用次数: 0

摘要

背景：口腔是葡萄球菌定植的生态位，而葡萄球菌是导致人类社区获得性感染的主要细菌种类。本研究调查了日本北部健康人口腔和皮肤中葡萄球菌的流行率和特征：方法：收集口腔中的唾液和手部皮肤表面的拭子，并在选择性培养基上进行培养。方法：采集口腔中的唾液和手部皮肤表面的拭子，在选择性培养基上进行培养，通过基因鉴定分离物的种类，并确定金黄色葡萄球菌和银色葡萄球菌的 ST。通过 PCR 检测与抗菌药耐药性相关的基因：结果：在 166 名参与者中，61 人（37%）共分离出 75 株金葡菌，其中口腔（48 株）比皮肤（27 株）更常见。在金黄色葡萄球菌分离物中发现的 23 种 ST 中，ST8（CC8）、ST15（CC15）和 ST188（CC1）最为常见（各 10 例），其中以 CC1 ST 为主（17 例）。在两个人的皮肤中分离到耐甲氧西林金黄色葡萄球菌（MRSA），属于 ST1 和 ST6。对红霉素和庆大霉素的耐药性分别与erm(A)和aac(6')-Ie-aph(2")-Ia有关，更常见于ST5和ST8分离株。一名参试者（0.6%）的口腔中检出了一个 S. argenteus 分离物（ST2250，mecA 阴性）。从 102 名参与者体内共分离出 186 株凝固酶阴性葡萄球菌（CoNS），经鉴定分为 14 个种，其中最常见的是华氏葡萄球菌（52 株），其次是头癣葡萄球菌（42 株）、无患子葡萄球菌（42 株）、嗜酸性葡萄球菌（52 株）和嗜碱性葡萄球菌（52 株）。在沙雷氏菌、溶血性沙雷氏菌和卡普拉氏菌中检测到 MecA，而精氨酸代谢移动元素（ACME）仅在卡普拉氏菌和表皮葡萄球菌中检测到：结论：金黄色葡萄球菌在口腔中的流行率高于皮肤表面，属于三大ST，其中CC1是优势菌系。抗菌药耐药性的流行程度因 CoNS 的种类而异。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Prevalence, genetic characteristics, and antimicrobial resistance of staphylococcal isolates from oral cavity and skin surface of healthy individuals in northern Japan

Background

Oral cavity is an ecological niche for colonization of staphylococci, which are a major bacterial species causing community-acquired infections in humans. In this study, prevalence, and characteristics of staphylococci in oral cavity and skin of healthy individuals were investigated in northern Japan.

Methods

Saliva from oral cavity and swab from skin surface of hand were collected and cultured on selective media. Species of the isolates were identified genetically, and ST was determined for S. aureus and S. argenteus. Genes associated with antimicrobial resistance were detected by PCR.

Results

Among 166 participants, a total of 75 S. aureus isolates were obtained from 61 individuals (37 %), and recovered more frequently in oral cavity (n = 48) than skin (n = 27). Among 23 STs identified in S. aureus isolates, ST8 (CC8), ST15 (CC15), and ST188 (CC1) were the most common (10 isolates each), with STs of CC1 being dominant (17 isolates). Methicillin-resistant S. aureus (MRSA) was isolated in the skin of two individuals and belonged to ST1 and ST6. Resistance to erythromycin and gentamicin associated with erm(A) and aac(6’)-Ie-aph(2”)-Ia, respectively, was more commonly found in ST5 and ST8 isolates. One S. argenteus isolate (ST2250, mecA-negative) was recovered from oral cavity of a participant (0.6 %). A total of 186 isolates of coagulase-negative staphylococci (CoNS) were recovered from 102 participants and identified into 14 species, with S. warneri being the most common (n = 52), followed by S. capitis (n = 42), S. saprophyticus (n = 20) and S. haemolyticus (n = 19). mecA was detected in S. saprophyticus, S. haemolyticus, and S. caprae, while arginine-catabolic mobile element (ACME) in only S. capitis and S. epidermidis.

Conclusion

S. aureus was more prevalent in oral cavity than skin surface, belonging to three major STs, with CC1 being a dominant lineage. The prevalence of antimicrobial resistance was distinct depending on CoNS species.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Journal of Infection and Public Health PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH -INFECTIOUS DISEASES

CiteScore

13.10

自引率

1.50%

发文量

203

审稿时长

96 days

期刊介绍： The Journal of Infection and Public Health, first official journal of the Saudi Arabian Ministry of National Guard Health Affairs, King Saud Bin Abdulaziz University for Health Sciences and the Saudi Association for Public Health, aims to be the foremost scientific, peer-reviewed journal encompassing infection prevention and control, microbiology, infectious diseases, public health and the application of healthcare epidemiology to the evaluation of health outcomes. The point of view of the journal is that infection and public health are closely intertwined and that advances in one area will have positive consequences on the other. The journal will be useful to all health professionals who are partners in the management of patients with communicable diseases, keeping them up to date. The journal is proud to have an international and diverse editorial board that will assist and facilitate the publication of articles that reflect a global view on infection control and public health, as well as emphasizing our focus on supporting the needs of public health practitioners. It is our aim to improve healthcare by reducing risk of infection and related adverse outcomes by critical review, selection, and dissemination of new and relevant information in the field of infection control, public health and infectious diseases in all healthcare settings and the community.