Ahmed Baligh Laaribi, Asma Mehri, Hamza Ben Yahia, Houda Chaouch, Wafa Babay, Amel Letaief, Hadda-Imene Ouzari, Naila Hannachi, Jalel Boukadida, Ines Zidi
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The HLA-G + 3142 C > G polymorphism was analysed by PCR-RFLP in 242 patients with chronic HBV infection (116 males and 126 females), 241 healthy controls (116 males and 125 females), and 100 spontaneously resolved subjects (52 males and 48 females). Patients with chronic HBV infection showed a higher frequency of the + 3142G allele compared to healthy controls and spontaneously resolved subjects (p = 0.001 and p = 0.002, respectively). An association between the + 3142G allele and high HBV DNA levels was observed when HBV patients were stratified based on their HBV DNA levels (p = 0.016). Furthermore, the dominant model (GG + GC vs CC) was associated with liver function parameters, including AST, ALT, and high HBV DNA levels (p = 0.04, p < 0.001 and p = 0.002, respectively). However, there was no significant association found between this polymorphism and the fibrosis stage (p = 0.32). The haplotype analysis, using a subset of previously published data on the HLA-G 14-pb Ins/Del polymorphism, revealed an association between the Ins/G haplotype and chronic HBV infection (H1: InsG, p < 0.001). Our findings suggest that the + 3142G allele is a risk factor for the persistence and progression of HBV infection, while the + 3142C allele serves as a protective allele associated with the spontaneous resolution of the infection. Additionally, the HLA-G 3'UTR haplotype Ins/G is associated with chronic HBV infection in the Tunisian population.</p>","PeriodicalId":13389,"journal":{"name":"Immunologic Research","volume":" ","pages":"1136-1146"},"PeriodicalIF":3.3000,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Association of HLA-G 3'UTR polymorphisms with hepatitis B virus infection in Tunisian population.\",\"authors\":\"Ahmed Baligh Laaribi, Asma Mehri, Hamza Ben Yahia, Houda Chaouch, Wafa Babay, Amel Letaief, Hadda-Imene Ouzari, Naila Hannachi, Jalel Boukadida, Ines Zidi\",\"doi\":\"10.1007/s12026-024-09516-2\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Hepatitis B virus (HBV) infection is a major public health burden. The mechanisms of immune evasion during chronic HBV (CHB) infection are poorly understood. 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引用次数: 0
摘要
乙型肝炎病毒(HBV)感染是一项重大的公共卫生负担。人们对慢性 HBV(CHB)感染期间的免疫逃避机制知之甚少。人类白细胞抗原(HLA)-G 是一种免疫检查点分子,在各种传染性疾病的耐受机制中发挥着至关重要的作用。3' 非翻译区(3'UTR),包括 HLA-G + 3142 C > G 多态性(rs1063320)和 14-pb Ins/Del(rs66554220),被强烈认为会影响 HLA-G 的表达。本研究进行了一项病例对照分析,以评估突尼斯队列中 HLA-G + 3142 C > G 多态性与 HBV 感染结果之间的潜在相关性。通过 PCR-RFLP 分析了 242 名慢性 HBV 感染者(男性 116 人,女性 126 人)、241 名健康对照者(男性 116 人,女性 125 人)和 100 名自发缓解者(男性 52 人,女性 48 人)的 HLA-G + 3142 C > G 多态性。与健康对照组和自发缓解的受试者相比,慢性 HBV 感染患者的 + 3142G 等位基因频率更高(分别为 p = 0.001 和 p = 0.002)。当根据 HBV 患者的 HBV DNA 水平进行分层时,观察到 + 3142G 等位基因与高 HBV DNA 水平之间存在关联(p = 0.016)。此外,显性模型(GG + GC vs CC)与肝功能参数(包括谷草转氨酶(AST)、谷丙转氨酶(ALT)和高 HBV DNA 水平)相关(p = 0.04,p = 0.002)。
Association of HLA-G 3'UTR polymorphisms with hepatitis B virus infection in Tunisian population.
Hepatitis B virus (HBV) infection is a major public health burden. The mechanisms of immune evasion during chronic HBV (CHB) infection are poorly understood. Human leukocyte antigen (HLA)-G, an immune checkpoint molecule, plays a crucial role in the tolerance mechanisms of various infectious diseases. The 3' untranslated region (3'UTR), including the HLA-G + 3142 C > G polymorphism (rs1063320) and the 14-pb Ins/Del (rs66554220) has been strongly suggested to influence HLA-G expression. This study conducted a case-control analysis to evaluate the potential correlation between the HLA-G + 3142 C > G polymorphism and HBV infection outcome in a Tunisian cohort. The HLA-G + 3142 C > G polymorphism was analysed by PCR-RFLP in 242 patients with chronic HBV infection (116 males and 126 females), 241 healthy controls (116 males and 125 females), and 100 spontaneously resolved subjects (52 males and 48 females). Patients with chronic HBV infection showed a higher frequency of the + 3142G allele compared to healthy controls and spontaneously resolved subjects (p = 0.001 and p = 0.002, respectively). An association between the + 3142G allele and high HBV DNA levels was observed when HBV patients were stratified based on their HBV DNA levels (p = 0.016). Furthermore, the dominant model (GG + GC vs CC) was associated with liver function parameters, including AST, ALT, and high HBV DNA levels (p = 0.04, p < 0.001 and p = 0.002, respectively). However, there was no significant association found between this polymorphism and the fibrosis stage (p = 0.32). The haplotype analysis, using a subset of previously published data on the HLA-G 14-pb Ins/Del polymorphism, revealed an association between the Ins/G haplotype and chronic HBV infection (H1: InsG, p < 0.001). Our findings suggest that the + 3142G allele is a risk factor for the persistence and progression of HBV infection, while the + 3142C allele serves as a protective allele associated with the spontaneous resolution of the infection. Additionally, the HLA-G 3'UTR haplotype Ins/G is associated with chronic HBV infection in the Tunisian population.
期刊介绍:
IMMUNOLOGIC RESEARCH represents a unique medium for the presentation, interpretation, and clarification of complex scientific data. Information is presented in the form of interpretive synthesis reviews, original research articles, symposia, editorials, and theoretical essays. The scope of coverage extends to cellular immunology, immunogenetics, molecular and structural immunology, immunoregulation and autoimmunity, immunopathology, tumor immunology, host defense and microbial immunity, including viral immunology, immunohematology, mucosal immunity, complement, transplantation immunology, clinical immunology, neuroimmunology, immunoendocrinology, immunotoxicology, translational immunology, and history of immunology.