Toga Pangihotan Napitupulu , Ismu Purnaningsih , Atit Kanti , I Made Sudiana
{"title":"印度尼西亚茜草科植物根茎溶剂提取物化学成分和体外抗镰刀菌活性的比较评价","authors":"Toga Pangihotan Napitupulu , Ismu Purnaningsih , Atit Kanti , I Made Sudiana","doi":"10.1016/j.kjs.2024.100281","DOIUrl":null,"url":null,"abstract":"<div><p><em>Fusarium oxysporum</em> f.sp. <em>cubense</em> (Foc) is a fungus that not only causes disease while bananas are being grown, but it also causes begetting after bananas are harvested, which limits the production of bananas. <em>Zingiberaceae</em> plants contain a high number of extractable secondary metabolites that are regarded to have effective antifungal activities. However, in order for the compounds to be suitable for use, they must be tested by modifying the polarity in the extracting solvent. In this study, four <em>Zingiberaceae</em> species were examined, namely <em>Zingiber officinale</em>, <em>Curcuma longa</em>, <em>C</em>. <em>zanthorrhiza</em>, and <em>Alpinia galanga</em>. The extraction of rhizomes of each plant was accomplished by using solvents with distinct polarity index (PI) viz. water (PI 9.0); a mixture of 50% water and 50% ethanol (PI 7.1); ethanol (PI 5.2); and n-hexane (PI 0.0). The anti-<em>Fusarium</em> evaluation of these 16 extracts was carried out <em>in vitro</em> by implementing the pour plate method in potato dextrose agar (PDA). Our results showed that the <em>C. zanthorrhiza</em> n-hexane extract possessed the greatest antifungal activity among other extracts. The fungal inhibition ability of the extracts was negatively correlated with the increase in solvent polarity index. In order to comprehend our understanding of the antifungal activity, the chemical compositions of each extract were identified through non-targeted Gas Chromatography–Mass Spectrometry (GC-MS), showing the main components of the n-hexane extracts were terpenes and terpenoids. Particularly, <em>C. zanthorrhiza</em> n-hexane extract contained, with the percentage of abundance in parentheses, germacrene B (22.99%), β-farnesene (14.72%), curzerene (10.87%), 2-bornanone (9.60%), α-curcumene (9.32%), zingiberene (8.30%), β-curcumene (6.36%), β-elemene (5.31%), caryophyllene (3.36%), cedrene (2.73%), α-bergamotene (1.36%), and humulene (0.20%). As conclusion, this crude extract or its pure chemical constituents have potential application as biofumigant to control Foc in postharvest banana.</p></div>","PeriodicalId":17848,"journal":{"name":"Kuwait Journal of Science","volume":"51 4","pages":"Article 100281"},"PeriodicalIF":1.2000,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2307410824001068/pdfft?md5=a5fceecdab4646c83d3ffa0b8c50d41a&pid=1-s2.0-S2307410824001068-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Comparative evaluation on chemical composition and in vitro anti-Fusarium activity of solvent extracts of Zingiberaceae rhizomes from Indonesia\",\"authors\":\"Toga Pangihotan Napitupulu , Ismu Purnaningsih , Atit Kanti , I Made Sudiana\",\"doi\":\"10.1016/j.kjs.2024.100281\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><em>Fusarium oxysporum</em> f.sp. <em>cubense</em> (Foc) is a fungus that not only causes disease while bananas are being grown, but it also causes begetting after bananas are harvested, which limits the production of bananas. <em>Zingiberaceae</em> plants contain a high number of extractable secondary metabolites that are regarded to have effective antifungal activities. However, in order for the compounds to be suitable for use, they must be tested by modifying the polarity in the extracting solvent. In this study, four <em>Zingiberaceae</em> species were examined, namely <em>Zingiber officinale</em>, <em>Curcuma longa</em>, <em>C</em>. <em>zanthorrhiza</em>, and <em>Alpinia galanga</em>. The extraction of rhizomes of each plant was accomplished by using solvents with distinct polarity index (PI) viz. water (PI 9.0); a mixture of 50% water and 50% ethanol (PI 7.1); ethanol (PI 5.2); and n-hexane (PI 0.0). The anti-<em>Fusarium</em> evaluation of these 16 extracts was carried out <em>in vitro</em> by implementing the pour plate method in potato dextrose agar (PDA). Our results showed that the <em>C. zanthorrhiza</em> n-hexane extract possessed the greatest antifungal activity among other extracts. The fungal inhibition ability of the extracts was negatively correlated with the increase in solvent polarity index. In order to comprehend our understanding of the antifungal activity, the chemical compositions of each extract were identified through non-targeted Gas Chromatography–Mass Spectrometry (GC-MS), showing the main components of the n-hexane extracts were terpenes and terpenoids. Particularly, <em>C. zanthorrhiza</em> n-hexane extract contained, with the percentage of abundance in parentheses, germacrene B (22.99%), β-farnesene (14.72%), curzerene (10.87%), 2-bornanone (9.60%), α-curcumene (9.32%), zingiberene (8.30%), β-curcumene (6.36%), β-elemene (5.31%), caryophyllene (3.36%), cedrene (2.73%), α-bergamotene (1.36%), and humulene (0.20%). As conclusion, this crude extract or its pure chemical constituents have potential application as biofumigant to control Foc in postharvest banana.</p></div>\",\"PeriodicalId\":17848,\"journal\":{\"name\":\"Kuwait Journal of Science\",\"volume\":\"51 4\",\"pages\":\"Article 100281\"},\"PeriodicalIF\":1.2000,\"publicationDate\":\"2024-07-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2307410824001068/pdfft?md5=a5fceecdab4646c83d3ffa0b8c50d41a&pid=1-s2.0-S2307410824001068-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Kuwait Journal of Science\",\"FirstCategoryId\":\"103\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2307410824001068\",\"RegionNum\":4,\"RegionCategory\":\"综合性期刊\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"MULTIDISCIPLINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Kuwait Journal of Science","FirstCategoryId":"103","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2307410824001068","RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MULTIDISCIPLINARY SCIENCES","Score":null,"Total":0}
Comparative evaluation on chemical composition and in vitro anti-Fusarium activity of solvent extracts of Zingiberaceae rhizomes from Indonesia
Fusarium oxysporum f.sp. cubense (Foc) is a fungus that not only causes disease while bananas are being grown, but it also causes begetting after bananas are harvested, which limits the production of bananas. Zingiberaceae plants contain a high number of extractable secondary metabolites that are regarded to have effective antifungal activities. However, in order for the compounds to be suitable for use, they must be tested by modifying the polarity in the extracting solvent. In this study, four Zingiberaceae species were examined, namely Zingiber officinale, Curcuma longa, C. zanthorrhiza, and Alpinia galanga. The extraction of rhizomes of each plant was accomplished by using solvents with distinct polarity index (PI) viz. water (PI 9.0); a mixture of 50% water and 50% ethanol (PI 7.1); ethanol (PI 5.2); and n-hexane (PI 0.0). The anti-Fusarium evaluation of these 16 extracts was carried out in vitro by implementing the pour plate method in potato dextrose agar (PDA). Our results showed that the C. zanthorrhiza n-hexane extract possessed the greatest antifungal activity among other extracts. The fungal inhibition ability of the extracts was negatively correlated with the increase in solvent polarity index. In order to comprehend our understanding of the antifungal activity, the chemical compositions of each extract were identified through non-targeted Gas Chromatography–Mass Spectrometry (GC-MS), showing the main components of the n-hexane extracts were terpenes and terpenoids. Particularly, C. zanthorrhiza n-hexane extract contained, with the percentage of abundance in parentheses, germacrene B (22.99%), β-farnesene (14.72%), curzerene (10.87%), 2-bornanone (9.60%), α-curcumene (9.32%), zingiberene (8.30%), β-curcumene (6.36%), β-elemene (5.31%), caryophyllene (3.36%), cedrene (2.73%), α-bergamotene (1.36%), and humulene (0.20%). As conclusion, this crude extract or its pure chemical constituents have potential application as biofumigant to control Foc in postharvest banana.
期刊介绍:
Kuwait Journal of Science (KJS) is indexed and abstracted by major publishing houses such as Chemical Abstract, Science Citation Index, Current contents, Mathematics Abstract, Micribiological Abstracts etc. KJS publishes peer-review articles in various fields of Science including Mathematics, Computer Science, Physics, Statistics, Biology, Chemistry and Earth & Environmental Sciences. In addition, it also aims to bring the results of scientific research carried out under a variety of intellectual traditions and organizations to the attention of specialized scholarly readership. As such, the publisher expects the submission of original manuscripts which contain analysis and solutions about important theoretical, empirical and normative issues.