印度尼西亚茜草科植物根茎溶剂提取物化学成分和体外抗镰刀菌活性的比较评价

IF 1.2 4区 综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES
Toga Pangihotan Napitupulu , Ismu Purnaningsih , Atit Kanti , I Made Sudiana
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引用次数: 0

摘要

Fusarium oxysporum f.sp. cubense(Foc)是一种真菌,它不仅会在香蕉生长过程中引起病害,还会在香蕉收获后引起生育,从而限制香蕉的产量。人参科植物含有大量可提取的次生代谢物,被认为具有有效的抗真菌活性。然而,为了使这些化合物适合使用,必须通过改变萃取溶剂的极性对其进行测试。本研究考察了四种姜科植物,即 Zingiber officinale、Curcuma longa、C. zanthorrhiza 和 Alpinia galanga。每种植物根茎的提取均采用不同极性指数(PI)的溶剂,即水(PI 9.0)、50% 水和 50%乙醇的混合物(PI 7.1)、乙醇(PI 5.2)和正己烷(PI 0.0)。通过在马铃薯葡萄糖琼脂(PDA)中采用浇板法,对这 16 种提取物进行了体外抗镰刀菌评价。结果表明,在其他提取物中,C. zanthorrhiza 正己烷提取物具有最强的抗真菌活性。提取物的抑菌能力与溶剂极性指数的增加呈负相关。为了更好地理解这些提取物的抗真菌活性,我们对每种提取物的化学成分进行了非目标气相色谱-质谱(GC-MS)鉴定,结果表明正己烷提取物的主要成分是萜烯和萜类化合物。特别是,C. zanthorrhiza 正己烷提取物中(括号内为丰度百分比)含有胚芽烯 B(22.99%)、β-法呢烯(14.72%)、莪术烯(10.87%)、2-龙脑酮(9.60%), α-curcumene (9.32%), zingiberene (8.30%), β-curcumene (6.36%), β-elemene (5.31%), caryophyllene (3.36%), cedrene (2.73%), α-bergamotene (1.36%), and humulene (0.20%).总之,这种粗提取物或其纯化学成分有可能用作生物熏蒸剂来控制收获后香蕉中的虫害。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comparative evaluation on chemical composition and in vitro anti-Fusarium activity of solvent extracts of Zingiberaceae rhizomes from Indonesia

Fusarium oxysporum f.sp. cubense (Foc) is a fungus that not only causes disease while bananas are being grown, but it also causes begetting after bananas are harvested, which limits the production of bananas. Zingiberaceae plants contain a high number of extractable secondary metabolites that are regarded to have effective antifungal activities. However, in order for the compounds to be suitable for use, they must be tested by modifying the polarity in the extracting solvent. In this study, four Zingiberaceae species were examined, namely Zingiber officinale, Curcuma longa, C. zanthorrhiza, and Alpinia galanga. The extraction of rhizomes of each plant was accomplished by using solvents with distinct polarity index (PI) viz. water (PI 9.0); a mixture of 50% water and 50% ethanol (PI 7.1); ethanol (PI 5.2); and n-hexane (PI 0.0). The anti-Fusarium evaluation of these 16 extracts was carried out in vitro by implementing the pour plate method in potato dextrose agar (PDA). Our results showed that the C. zanthorrhiza n-hexane extract possessed the greatest antifungal activity among other extracts. The fungal inhibition ability of the extracts was negatively correlated with the increase in solvent polarity index. In order to comprehend our understanding of the antifungal activity, the chemical compositions of each extract were identified through non-targeted Gas Chromatography–Mass Spectrometry (GC-MS), showing the main components of the n-hexane extracts were terpenes and terpenoids. Particularly, C. zanthorrhiza n-hexane extract contained, with the percentage of abundance in parentheses, germacrene B (22.99%), β-farnesene (14.72%), curzerene (10.87%), 2-bornanone (9.60%), α-curcumene (9.32%), zingiberene (8.30%), β-curcumene (6.36%), β-elemene (5.31%), caryophyllene (3.36%), cedrene (2.73%), α-bergamotene (1.36%), and humulene (0.20%). As conclusion, this crude extract or its pure chemical constituents have potential application as biofumigant to control Foc in postharvest banana.

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来源期刊
Kuwait Journal of Science
Kuwait Journal of Science MULTIDISCIPLINARY SCIENCES-
CiteScore
1.60
自引率
28.60%
发文量
132
期刊介绍: Kuwait Journal of Science (KJS) is indexed and abstracted by major publishing houses such as Chemical Abstract, Science Citation Index, Current contents, Mathematics Abstract, Micribiological Abstracts etc. KJS publishes peer-review articles in various fields of Science including Mathematics, Computer Science, Physics, Statistics, Biology, Chemistry and Earth & Environmental Sciences. In addition, it also aims to bring the results of scientific research carried out under a variety of intellectual traditions and organizations to the attention of specialized scholarly readership. As such, the publisher expects the submission of original manuscripts which contain analysis and solutions about important theoretical, empirical and normative issues.
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