Sirirak Hemmaphan , Kotchapawn Somprasong , Narisa K. Bordeerat
{"title":"利用人体气道上皮细胞模型评估纳米颗粒的环境遗传毒性","authors":"Sirirak Hemmaphan , Kotchapawn Somprasong , Narisa K. Bordeerat","doi":"10.1016/j.emcon.2024.100386","DOIUrl":null,"url":null,"abstract":"<div><p>Because of the growing use of nanoparticles (NPs) in manufacturing consumer goods, it is essential to identify new <em>in vitro</em> approaches to assess their toxicity and improve risk assessment. Humans are exposed to NPs from contaminated aerosol via multiple routes, such as inhalation, ingestion, and dermal absorption. Human airway epithelial cells are a promising tool for assessing the genotoxicity of NPs <em>in vitro</em>. This study aims to evaluate the genotoxic effects of titanium dioxide (TiO<sub>2</sub>), zinc oxide (ZnO), and silver oxide (AgO) NPs on human bronchial/tracheal epithelial cells (HBTECs) using the comet assay. The exposure duration was set to 24 h, and two- (2D) and three-dimensional (3D) spheroid models were used. The genotoxicity of TiO<sub>2</sub> NPs in 2D and 3D HBTEC cells was not statistically significant based on the concentration points examined <em>in vitro</em>. ZnO NPs at concentrations ranging from 10 to 50 μg/mL, when exposed to 2D HBTEC cultures for 24 h, did not show any genotoxic effects. However, genotoxicity was observed at higher concentrations. In contrast, treatment of 2D HBTEC cultures with AgO NPs increased the percentage of tail DNA damage from 50 to 100 μg/mL in a concentration-dependent manner. Within a controlled laboratory environment, ZnO and AgO NPs exhibited a reduction in the length of the tail in 3D spheroid cells. When evaluating genotoxicity, spheroids (3D) represent <em>in vivo</em>-like cell behavior more accurately than monolayer cultures (2D). Collectively, our findings illustrate the harmful effects of NPs on genetic material and emphasize the significance of employing cell culture models to evaluate the risk of toxicity.</p></div>","PeriodicalId":11539,"journal":{"name":"Emerging Contaminants","volume":"10 4","pages":"Article 100386"},"PeriodicalIF":5.3000,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2405665024000878/pdfft?md5=a3503e6b0acd0c962874a059db274da1&pid=1-s2.0-S2405665024000878-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Environmental genotoxicity assessment of nanoparticles using human airway epithelial model\",\"authors\":\"Sirirak Hemmaphan , Kotchapawn Somprasong , Narisa K. Bordeerat\",\"doi\":\"10.1016/j.emcon.2024.100386\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Because of the growing use of nanoparticles (NPs) in manufacturing consumer goods, it is essential to identify new <em>in vitro</em> approaches to assess their toxicity and improve risk assessment. Humans are exposed to NPs from contaminated aerosol via multiple routes, such as inhalation, ingestion, and dermal absorption. Human airway epithelial cells are a promising tool for assessing the genotoxicity of NPs <em>in vitro</em>. This study aims to evaluate the genotoxic effects of titanium dioxide (TiO<sub>2</sub>), zinc oxide (ZnO), and silver oxide (AgO) NPs on human bronchial/tracheal epithelial cells (HBTECs) using the comet assay. The exposure duration was set to 24 h, and two- (2D) and three-dimensional (3D) spheroid models were used. The genotoxicity of TiO<sub>2</sub> NPs in 2D and 3D HBTEC cells was not statistically significant based on the concentration points examined <em>in vitro</em>. ZnO NPs at concentrations ranging from 10 to 50 μg/mL, when exposed to 2D HBTEC cultures for 24 h, did not show any genotoxic effects. However, genotoxicity was observed at higher concentrations. In contrast, treatment of 2D HBTEC cultures with AgO NPs increased the percentage of tail DNA damage from 50 to 100 μg/mL in a concentration-dependent manner. Within a controlled laboratory environment, ZnO and AgO NPs exhibited a reduction in the length of the tail in 3D spheroid cells. When evaluating genotoxicity, spheroids (3D) represent <em>in vivo</em>-like cell behavior more accurately than monolayer cultures (2D). Collectively, our findings illustrate the harmful effects of NPs on genetic material and emphasize the significance of employing cell culture models to evaluate the risk of toxicity.</p></div>\",\"PeriodicalId\":11539,\"journal\":{\"name\":\"Emerging Contaminants\",\"volume\":\"10 4\",\"pages\":\"Article 100386\"},\"PeriodicalIF\":5.3000,\"publicationDate\":\"2024-07-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2405665024000878/pdfft?md5=a3503e6b0acd0c962874a059db274da1&pid=1-s2.0-S2405665024000878-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Emerging Contaminants\",\"FirstCategoryId\":\"1087\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2405665024000878\",\"RegionNum\":2,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENVIRONMENTAL SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Emerging Contaminants","FirstCategoryId":"1087","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2405665024000878","RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
Environmental genotoxicity assessment of nanoparticles using human airway epithelial model
Because of the growing use of nanoparticles (NPs) in manufacturing consumer goods, it is essential to identify new in vitro approaches to assess their toxicity and improve risk assessment. Humans are exposed to NPs from contaminated aerosol via multiple routes, such as inhalation, ingestion, and dermal absorption. Human airway epithelial cells are a promising tool for assessing the genotoxicity of NPs in vitro. This study aims to evaluate the genotoxic effects of titanium dioxide (TiO2), zinc oxide (ZnO), and silver oxide (AgO) NPs on human bronchial/tracheal epithelial cells (HBTECs) using the comet assay. The exposure duration was set to 24 h, and two- (2D) and three-dimensional (3D) spheroid models were used. The genotoxicity of TiO2 NPs in 2D and 3D HBTEC cells was not statistically significant based on the concentration points examined in vitro. ZnO NPs at concentrations ranging from 10 to 50 μg/mL, when exposed to 2D HBTEC cultures for 24 h, did not show any genotoxic effects. However, genotoxicity was observed at higher concentrations. In contrast, treatment of 2D HBTEC cultures with AgO NPs increased the percentage of tail DNA damage from 50 to 100 μg/mL in a concentration-dependent manner. Within a controlled laboratory environment, ZnO and AgO NPs exhibited a reduction in the length of the tail in 3D spheroid cells. When evaluating genotoxicity, spheroids (3D) represent in vivo-like cell behavior more accurately than monolayer cultures (2D). Collectively, our findings illustrate the harmful effects of NPs on genetic material and emphasize the significance of employing cell culture models to evaluate the risk of toxicity.
期刊介绍:
Emerging Contaminants is an outlet for world-leading research addressing problems associated with environmental contamination caused by emerging contaminants and their solutions. Emerging contaminants are defined as chemicals that are not currently (or have been only recently) regulated and about which there exist concerns regarding their impact on human or ecological health. Examples of emerging contaminants include disinfection by-products, pharmaceutical and personal care products, persistent organic chemicals, and mercury etc. as well as their degradation products. We encourage papers addressing science that facilitates greater understanding of the nature, extent, and impacts of the presence of emerging contaminants in the environment; technology that exploits original principles to reduce and control their environmental presence; as well as the development, implementation and efficacy of national and international policies to protect human health and the environment from emerging contaminants.