Adama Patrice Soubeiga , Dissinviel Stéphane Kpoda , Oumar Traoré , Cheikna Zongo , Aly Savadogo
{"title":"布基纳法索瓦加杜古食品样本中发现的耐多药大肠埃希菌和沙门氏菌中的β-内酰胺酶基因和整合子的流行情况","authors":"Adama Patrice Soubeiga , Dissinviel Stéphane Kpoda , Oumar Traoré , Cheikna Zongo , Aly Savadogo","doi":"10.1016/j.sciaf.2024.e02302","DOIUrl":null,"url":null,"abstract":"<div><p>The resistance of Gram-negative bacteria to β-lactam antibiotics occurs mainly by the production of β-lactamases. The aim of this work was to evaluate the presence of β-lactamase genes and integrons in multidrug-resistant (MDR) isolates recovered from food. Susceptibility to antibiotics was determined using the agar diffusion method. Detection of β-lactam resistance genes and integrons was performed with the 53 confirmed MDR strains (42 <em>E. coli</em> and 11 <em>Salmonella</em> spp.) by PCR. High resistance rates of 92.5 %, 60.4 %, and 54.7 % against ampicillin, tetracycline, and amoxicillin/clavulanic acid were observed, respectively. Two β-lactamase genes <em>bla<sub>TEM</sub></em> (37.7 %) and <em>bla<sub>SHV</sub></em> (28.3) were detected among <em>E. coli</em> and <em>Salmonella</em> isolates. High rate of isolates (85 %) exhibited class 1 integrons was observed. These isolates can act as sources of antimicrobial resistance genes and promote the spread of these resistance determinants to other commensal and pathogenic bacteria. Therefore, preventive measures and clean environments are recommended to prevent food contamination with resistant microorganisms.</p></div>","PeriodicalId":21690,"journal":{"name":"Scientific African","volume":null,"pages":null},"PeriodicalIF":2.7000,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2468227624002461/pdfft?md5=36ae86ba8b28ce61df476899defa4d40&pid=1-s2.0-S2468227624002461-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Prevalence of β-lactamase genes and integrons among multidrug-resistant Escherichia coli and Salmonella spp recovered from food samples in Ouagadougou, Burkina Faso\",\"authors\":\"Adama Patrice Soubeiga , Dissinviel Stéphane Kpoda , Oumar Traoré , Cheikna Zongo , Aly Savadogo\",\"doi\":\"10.1016/j.sciaf.2024.e02302\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The resistance of Gram-negative bacteria to β-lactam antibiotics occurs mainly by the production of β-lactamases. The aim of this work was to evaluate the presence of β-lactamase genes and integrons in multidrug-resistant (MDR) isolates recovered from food. Susceptibility to antibiotics was determined using the agar diffusion method. Detection of β-lactam resistance genes and integrons was performed with the 53 confirmed MDR strains (42 <em>E. coli</em> and 11 <em>Salmonella</em> spp.) by PCR. High resistance rates of 92.5 %, 60.4 %, and 54.7 % against ampicillin, tetracycline, and amoxicillin/clavulanic acid were observed, respectively. Two β-lactamase genes <em>bla<sub>TEM</sub></em> (37.7 %) and <em>bla<sub>SHV</sub></em> (28.3) were detected among <em>E. coli</em> and <em>Salmonella</em> isolates. High rate of isolates (85 %) exhibited class 1 integrons was observed. These isolates can act as sources of antimicrobial resistance genes and promote the spread of these resistance determinants to other commensal and pathogenic bacteria. Therefore, preventive measures and clean environments are recommended to prevent food contamination with resistant microorganisms.</p></div>\",\"PeriodicalId\":21690,\"journal\":{\"name\":\"Scientific African\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.7000,\"publicationDate\":\"2024-07-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2468227624002461/pdfft?md5=36ae86ba8b28ce61df476899defa4d40&pid=1-s2.0-S2468227624002461-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Scientific African\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2468227624002461\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MULTIDISCIPLINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Scientific African","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2468227624002461","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MULTIDISCIPLINARY SCIENCES","Score":null,"Total":0}
Prevalence of β-lactamase genes and integrons among multidrug-resistant Escherichia coli and Salmonella spp recovered from food samples in Ouagadougou, Burkina Faso
The resistance of Gram-negative bacteria to β-lactam antibiotics occurs mainly by the production of β-lactamases. The aim of this work was to evaluate the presence of β-lactamase genes and integrons in multidrug-resistant (MDR) isolates recovered from food. Susceptibility to antibiotics was determined using the agar diffusion method. Detection of β-lactam resistance genes and integrons was performed with the 53 confirmed MDR strains (42 E. coli and 11 Salmonella spp.) by PCR. High resistance rates of 92.5 %, 60.4 %, and 54.7 % against ampicillin, tetracycline, and amoxicillin/clavulanic acid were observed, respectively. Two β-lactamase genes blaTEM (37.7 %) and blaSHV (28.3) were detected among E. coli and Salmonella isolates. High rate of isolates (85 %) exhibited class 1 integrons was observed. These isolates can act as sources of antimicrobial resistance genes and promote the spread of these resistance determinants to other commensal and pathogenic bacteria. Therefore, preventive measures and clean environments are recommended to prevent food contamination with resistant microorganisms.
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