Yonghong Huang , Jinghui Li , Xiaoying Shan , Hanting Wang , Yanxin Duan
{"title":"内生细菌菌株芽孢杆菌(Bacillus tequilensis QNF2)抑制采后苹果果实上由多蒂腐霉菌(Botryosphaeria dothidea)引起的苹果环腐病的生物活性评估","authors":"Yonghong Huang , Jinghui Li , Xiaoying Shan , Hanting Wang , Yanxin Duan","doi":"10.1016/j.fm.2024.104590","DOIUrl":null,"url":null,"abstract":"<div><p>Apple ring rot, one of the most common apple postharvest diseases during storage, is caused by <em>Botryosphaeria dothidea</em>. Presently, the disease management is primarily dependent on chemical fungicide application. Here we demonstrated an endophyte bacterium <em>Bacillus tequilensis</em> QNF2, isolated from Chinese leek (<em>Allium tuberosum</em>) roots considerably suppressed <em>B. dothidea</em> mycelial growth, with the highest suppression of 73.56 % and 99.5 % in the PDA and PDB medium, respectively in vitro confront experiments. In in vivo experiments, <em>B. tequilensis</em> QNF2 exhibited a control efficacy of 88.52 % and 100 % on ring rot disease on postharvest apple fruits inoculated with <em>B. dothidea</em> disc and dipped into <em>B. dothidea</em> culture, respectively. In addition, <em>B. tequilensis</em> QNF2 volatile organic compounds (VOCs) also manifested markedly inhibition against <em>B. dothidea</em> mycelial growth and the ring rot on postharvest apple fruits. Moreover, <em>B. tequilensis</em> QNF2 severely damaged the mycelial morphology of <em>B. dothidea</em>. Finally, <em>B. tequilensis</em> QNF2 significantly repressed the expression of six pathogenicity-related genes, such as <em>adh</em>, <em>aldh</em>, <em>aldh3</em>, <em>galm</em>, <em>pdc1</em>, <em>pdc2</em>, involved in glycolysis/gluconeogenesis of <em>B. dothidea</em>. The findings of the study proved that <em>B. tequilensis</em> QNF2 was a promising alternative for controlling apple ring rot of postharvest apple fruit.</p></div>","PeriodicalId":12399,"journal":{"name":"Food microbiology","volume":"123 ","pages":"Article 104590"},"PeriodicalIF":4.5000,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Bioactivities evaluation of an endophytic bacterial strain Bacillus tequilensis QNF2 inhibiting apple ring rot caused by Botryosphaeria dothidea on postharvest apple fruits\",\"authors\":\"Yonghong Huang , Jinghui Li , Xiaoying Shan , Hanting Wang , Yanxin Duan\",\"doi\":\"10.1016/j.fm.2024.104590\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Apple ring rot, one of the most common apple postharvest diseases during storage, is caused by <em>Botryosphaeria dothidea</em>. Presently, the disease management is primarily dependent on chemical fungicide application. Here we demonstrated an endophyte bacterium <em>Bacillus tequilensis</em> QNF2, isolated from Chinese leek (<em>Allium tuberosum</em>) roots considerably suppressed <em>B. dothidea</em> mycelial growth, with the highest suppression of 73.56 % and 99.5 % in the PDA and PDB medium, respectively in vitro confront experiments. In in vivo experiments, <em>B. tequilensis</em> QNF2 exhibited a control efficacy of 88.52 % and 100 % on ring rot disease on postharvest apple fruits inoculated with <em>B. dothidea</em> disc and dipped into <em>B. dothidea</em> culture, respectively. In addition, <em>B. tequilensis</em> QNF2 volatile organic compounds (VOCs) also manifested markedly inhibition against <em>B. dothidea</em> mycelial growth and the ring rot on postharvest apple fruits. Moreover, <em>B. tequilensis</em> QNF2 severely damaged the mycelial morphology of <em>B. dothidea</em>. Finally, <em>B. tequilensis</em> QNF2 significantly repressed the expression of six pathogenicity-related genes, such as <em>adh</em>, <em>aldh</em>, <em>aldh3</em>, <em>galm</em>, <em>pdc1</em>, <em>pdc2</em>, involved in glycolysis/gluconeogenesis of <em>B. dothidea</em>. The findings of the study proved that <em>B. tequilensis</em> QNF2 was a promising alternative for controlling apple ring rot of postharvest apple fruit.</p></div>\",\"PeriodicalId\":12399,\"journal\":{\"name\":\"Food microbiology\",\"volume\":\"123 \",\"pages\":\"Article 104590\"},\"PeriodicalIF\":4.5000,\"publicationDate\":\"2024-07-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Food microbiology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S074000202400128X\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food microbiology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S074000202400128X","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Bioactivities evaluation of an endophytic bacterial strain Bacillus tequilensis QNF2 inhibiting apple ring rot caused by Botryosphaeria dothidea on postharvest apple fruits
Apple ring rot, one of the most common apple postharvest diseases during storage, is caused by Botryosphaeria dothidea. Presently, the disease management is primarily dependent on chemical fungicide application. Here we demonstrated an endophyte bacterium Bacillus tequilensis QNF2, isolated from Chinese leek (Allium tuberosum) roots considerably suppressed B. dothidea mycelial growth, with the highest suppression of 73.56 % and 99.5 % in the PDA and PDB medium, respectively in vitro confront experiments. In in vivo experiments, B. tequilensis QNF2 exhibited a control efficacy of 88.52 % and 100 % on ring rot disease on postharvest apple fruits inoculated with B. dothidea disc and dipped into B. dothidea culture, respectively. In addition, B. tequilensis QNF2 volatile organic compounds (VOCs) also manifested markedly inhibition against B. dothidea mycelial growth and the ring rot on postharvest apple fruits. Moreover, B. tequilensis QNF2 severely damaged the mycelial morphology of B. dothidea. Finally, B. tequilensis QNF2 significantly repressed the expression of six pathogenicity-related genes, such as adh, aldh, aldh3, galm, pdc1, pdc2, involved in glycolysis/gluconeogenesis of B. dothidea. The findings of the study proved that B. tequilensis QNF2 was a promising alternative for controlling apple ring rot of postharvest apple fruit.
期刊介绍:
Food Microbiology publishes original research articles, short communications, review papers, letters, news items and book reviews dealing with all aspects of the microbiology of foods. The editors aim to publish manuscripts of the highest quality which are both relevant and applicable to the broad field covered by the journal. Studies must be novel, have a clear connection to food microbiology, and be of general interest to the international community of food microbiologists. The editors make every effort to ensure rapid and fair reviews, resulting in timely publication of accepted manuscripts.