聚维酮碘的体外杀菌效果与接触时间、浓度、配制和储存时间的关系。

IF 2.4 3区 医学 Q2 OPHTHALMOLOGY
Christina M Ambrosino, Leo L Shen, Heba Mahjoub, Warda Memon, Sean X Zhang, Mark P Breazzano
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引用次数: 0

摘要

目的:尽管 5%聚维酮碘 (PVP-I) 经常被用作眼部抗菌剂,但对于 PVP-I 的浓度、开封后的储存以及复方制剂对 PVP-I 抗菌性的影响还缺乏共识。我们进行了一系列体外实验,以确定这些因素对 PVP-I 抑制术后眼部感染常见原因的影响:方法:在体外实验中,根据 PVP-I 活性暴露时间的函数来测量对微生物生长的抑制作用。在对照实验中,PVP-I 在微生物暴露前已失活。受测 PVP-I 溶液的浓度(0.6%、5% 或 10%)、开封后的储存时间(0、7 或 30 天)和制备方法(商用与从储备 PI 溶液中复配)各不相同。测试病原体包括表皮葡萄球菌、病毒性葡萄球菌、绿脓杆菌、耐甲氧西林金黄色葡萄球菌、甲氧西林敏感金黄色葡萄球菌和白念珠菌:与 5%的 PVP-I 相比,0.6%的 PVP-I 在抑制金黄色葡萄球菌生长方面效果较差(30 秒时为 200 ± 0 个菌落 vs. 7 ± 8 个菌落,P = 0.0004;1 分钟时为 183 ± 21 个菌落 vs. 0 ± 0 个菌落,P = 0.018),但在抑制铜绿假单胞菌方面效果较好(15 秒时为 30 ± 20 个菌落 vs. 200 ± 0 个菌落,P = 0.019)。与市售和新开封的 PVP-I 溶液相比,开瓶后存放 7 天或 30 天的复方制剂和溶液可以保持或提高对测试微生物的杀菌效果:结论:PVP-I 溶液的浓度会影响接触后 1 分钟内的杀菌效果,但所有溶液在 3 分钟内的杀菌效果相当。与之前研究稀释 PVP-I 的结果不同,0.6% PVP-I 的抗菌效果并不完全相同或更好。复配制剂和开瓶后的储存时间不会降低 PVP-I 的抗菌活性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Povidone-iodine in vitro antiseptic efficacy as a function of exposure duration, concentration, preparation, and length of storage.

Povidone-iodine in vitro antiseptic efficacy as a function of exposure duration, concentration, preparation, and length of storage.

Purpose: Although 5% povidone-iodine (PVP-I) is frequently used as an ocular antiseptic agent, there is a lack of consensus regarding the effects of PVP-I concentration, storage after opening, and compounded preparation on PVP-I antisepsis. We performed a series of in-vitro experiments to determine the impact of these factors on PVP-I's inhibition of common causes of post-procedural eye infection.

Methods: Inhibition of microorganism growth was measured in-vitro as a function of active PVP-I exposure time. In control experiments, PVP-I was inactivated before microorganism exposure. Tested PVP-I solutions varied in concentration (0.6%, 5%, or 10%), length of storage after opening (0, 7, or 30 days), and preparation (commercial vs.compounded from stock PI solution). Tested pathogens included S. epidermidis, S. viridans, P. aeruginosa, methicillin-resistant S. aureus, methicillin-sensitive S. aureus, and C. albicans.

Results: PVP-I solutions inhibited all bacterial growth by 3 min and fungal growth by 15 s. Compared to 5% PVP-I, the 0.6% PVP-I was less effective in inhibiting S. viridans growth (200 ± 0 colonies vs. 7 ± 8 at 30 s, P = 0.0004; 183 ± 21 vs. 0 ± 0 at 1 min, P = 0.018), but more effective in inhibiting P. aeruginosa (30 ± 20 vs. 200 ± 0 at 15 s, P = 0.019). Compared to commercial and newly-opened PVP-I solutions, compounded preparations and solutions stored for 7 or 30 days after bottle opening either preserved or improved antiseptic efficacy against tested microorganisms.

Conclusions: Concentration of PVP-I solution affects antiseptic efficacy within 1 min of exposure, but all solutions performed equivalently at 3 min. In contrast to results of prior studies investigating dilute PVP-I, the 0.6% PVP-I did not demonstrate a uniformly equivalent or superior anti-septic effect. Compounded preparation and storage length after bottle opening did not decrease PVP-I antiseptic activity.

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来源期刊
CiteScore
5.40
自引率
7.40%
发文量
398
审稿时长
3 months
期刊介绍: Graefe''s Archive for Clinical and Experimental Ophthalmology is a distinguished international journal that presents original clinical reports and clini-cally relevant experimental studies. Founded in 1854 by Albrecht von Graefe to serve as a source of useful clinical information and a stimulus for discussion, the journal has published articles by leading ophthalmologists and vision research scientists for more than a century. With peer review by an international Editorial Board and prompt English-language publication, Graefe''s Archive provides rapid dissemination of clinical and clinically related experimental information.
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