利用石墨烯涂层增强脱细胞脐带动脉的导电性和机械性能

IF 3.2 4区 医学 Q2 ENGINEERING, BIOMEDICAL
Fateme Karimi, Morteza Alizadeh, Fateme Sadat Bitaraf, Vahid Shirshahi
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引用次数: 0

摘要

传统的脱细胞生物支架具有完整的血管网络和独特的结构,作为修复神经损伤的管道已被广泛研究。然而,它们因缺乏导电性而受到限制,而导电性对神经组织的正常运作至关重要。本研究通过在脐带动脉内表面涂覆氧化石墨烯(GO)和还原氧化石墨烯(RGO),重点研究了脱细胞脐带动脉。这使得均匀的 GO 涂层完全覆盖了动脉的内腔。电学测量结果表明,加入 RGO 和 GO 导电片后,支架的导电性能显著增强。在 0.1 Hz 的低频下,与未涂覆的支架相比,涂覆了 RGO 的支架的电阻水平降低了 99.8%,涂覆了 GO 的支架的电阻水平降低了 98.21%。此外,脱细胞过程后,GO 和 RGO 的动脉机械性能分别提高了 24.69% 和 32.9%。GO 和 RGO 涂层不会影响内皮细胞的粘附性,并能促进细胞生长。细胞毒性测试表明,随着时间的推移,RGO 的细胞存活率上升,而 GO 的细胞存活率下降,这表明 GO 和 RGO 的细胞毒性受时间影响。血液相容性评估表明,石墨烯纳米材料不会诱发溶血,但有一定的血液凝固倾向。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Enhancing electrical conductivity and mechanical properties of decellularized umbilical cord arteries using graphene coatings

Enhancing electrical conductivity and mechanical properties of decellularized umbilical cord arteries using graphene coatings

Traditional decellularized bioscaffolds possessing intact vascular networks and unique architecture have been extensively studied as conduits for repairing nerve damage. However, they are limited by the absence of electrical conductivity, which is crucial for proper functioning of nervous tissue. This study focuses on investigating decellularized umbilical cord arteries by applying coatings of graphene oxide (GO) and reduced graphene oxide (RGO) to their inner surfaces. This resulted in a homogeneous GO coating that fully covered the internal lumen of the arteries. The results of electrical measurements demonstrated that the conductivity of the scaffolds could be significantly enhanced by incorporating RGO and GO conductive sheets. At a low frequency of 0.1 Hz, the electrical resistance level of the coated scaffolds decreased by 99.8% with RGO and 98.21% with GO, compared with uncoated scaffolds. Additionally, the mechanical properties of the arteries improved by 24.69% with GO and 32.9% with RGO after the decellularization process. The GO and RGO coatings did not compromise the adhesion of endothelial cells and promoted cell growth. The cytotoxicity tests revealed that cell survival rate increased over time with RGO, while it decreased with GO, indicating the time-dependent effect on the cytotoxicity of GO and RGO. Blood compatibility evaluations showed that graphene nanomaterials did not induce hemolysis but exhibited some tendency toward blood coagulation.

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来源期刊
CiteScore
7.50
自引率
2.90%
发文量
199
审稿时长
12 months
期刊介绍: Journal of Biomedical Materials Research – Part B: Applied Biomaterials is a highly interdisciplinary peer-reviewed journal serving the needs of biomaterials professionals who design, develop, produce and apply biomaterials and medical devices. It has the common focus of biomaterials applied to the human body and covers all disciplines where medical devices are used. Papers are published on biomaterials related to medical device development and manufacture, degradation in the body, nano- and biomimetic- biomaterials interactions, mechanics of biomaterials, implant retrieval and analysis, tissue-biomaterial surface interactions, wound healing, infection, drug delivery, standards and regulation of devices, animal and pre-clinical studies of biomaterials and medical devices, and tissue-biopolymer-material combination products. Manuscripts are published in one of six formats: • original research reports • short research and development reports • scientific reviews • current concepts articles • special reports • editorials Journal of Biomedical Materials Research – Part B: Applied Biomaterials is an official journal of the Society for Biomaterials, Japanese Society for Biomaterials, the Australasian Society for Biomaterials, and the Korean Society for Biomaterials. Manuscripts from all countries are invited but must be in English. Authors are not required to be members of the affiliated Societies, but members of these societies are encouraged to submit their work to the journal for consideration.
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