Zhenyu Yang, Jing Gao, Kai Tang, Longyan Duan, Shiqi Dai, An Chen, Wei Zhou, Jihua Chen
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引用次数: 0
摘要
目的:评估甲基丙烯酸氨基甲酯前体(UMP)对脱矿牙本质(DD)基质抗酶性的影响:配制含有溶于丙酮(Ace)溶液中的 0(对照组)、1 和 5 mmol/L UMP 的实验处理。然后将处理过的试样在 0.1 mg/mL 胶原酶溶液中保存 24 小时,之后评估其干质量损失和羟脯氨酸(HYP)释放情况。此外,还对每组试样的膨胀率进行了评估。使用场发射扫描电子显微镜(FE-SEM)观察了 UMP 与牙本质基质之间的相互作用。使用激光共聚焦扫描显微镜(CLSM)评估了牙本质中的内源性酶活性:与其他处理组相比,使用 1 mM 和 5 mM UMP-Ace 处理可显著降低 DD 基质的干质量损失、HYP 释放和膨胀率(p < 0.05)。FE-SEM 和 CLSM 观察结果表明,使用 UMP-Ace 处理可保护牙本质基质的结构,并降低牙本质-胶原网络中的孔隙率:结论:使用 1 mM 和 5 mM UMP-Ace 处理可保护 DD 基质免受胶原酶降解,在临床上可能有助于提高混合层的耐久性。
Evaluation of Monomer-containing Isocyanate Groups on Stabilizing Demineralized Dentin Matrix Against Bond Interface Degradation.
Purpose: To evaluate the effect of urethane methacrylate precursor (UMP) on the enzymatic resistance of demineralized dentin (DD) matrices.
Materials and methods: Experimental treatments containing 0 (control), 1, and 5 mmol/L UMP dissolved in an acetone (Ace) solution were formulated. Dentin matrix specimens were demineralized in vitro and immersed in the experimental treatments for 1 h. The treated specimens were then stored in 0.1 mg/mL collagenase solution for 24 h, after which their dry mass loss and hydroxyproline (HYP) release were assessed. The swelling ratios of specimens in each group were also evaluated. The interaction between UMP and the dentin matrix was observed using field-emission scanning electron microscopy (FE-SEM). Endogenous enzyme activity in dentin was evaluated using confocal laser scanning microscopy (CLSM).
Results: Compared with the other treatment groups, treatment with 1 mM and 5 mM UMP-Ace significantly decreased the dry mass loss, HYP release and swelling ratio of the DD matrix (p < 0.05). FE-SEM and CLSM observations showed that treatment with UMP-Ace protected the structure of the dentin matrix and decreased porosity within the dentin-collagen network.
Conclusion: Treatment with 1 mM and 5 mM UMP-Ace protects DD matrix against collagenase degradation and may be clinically useful for improving the durability of the hybrid layer.