瞬态、纳米尺度、液态分子组装的时代即将到来。

IF 6 2区 生物学 Q1 CELL BIOLOGY
Akihiro Kusumi , Taka A. Tsunoyama , Kenichi G.N. Suzuki , Takahiro K. Fujiwara , Amine Aladag
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引用次数: 0

摘要

这篇综述探讨了细胞通过质膜(PM)上瞬时、纳米级的液态分子组合体进行信号传递、通讯和粘附的动态机制。传统观点认为,稳定的固态分子复合物具有这些功能。然而,先进的成像技术发现,许多信号传导蛋白和支架蛋白只是短暂地停留在这些分子复合物中,而质膜上的微米级蛋白集合体,包括细胞粘附结构和突触,很可能是由纳米液体蛋白岛组成的群岛。借用液-液相分离形成微米尺度生物凝聚物的概念,我们提出这些纳米尺度的低聚物和集合体是由多种微弱但特异的分子相互作用促成的,这些相互作用往往涉及内在无序区域。来自单个纳米液体信号复合物的信号将以脉冲形式出现。单分子成像技术是表征 PM 上这些瞬时纳米液体集合体的关键技术,它表明人们正在转向一种模型,即相互作用的流动性是信号调节和整合的基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Transient, nano-scale, liquid-like molecular assemblies coming of age

Transient, nano-scale, liquid-like molecular assemblies coming of age

This review examines the dynamic mechanisms underlying cellular signaling, communication, and adhesion via transient, nano-scale, liquid-like molecular assemblies on the plasma membrane (PM). Traditional views posit that stable, solid-like molecular complexes perform these functions. However, advanced imaging reveals that many signaling and scaffolding proteins only briefly reside in these molecular complexes and that micron-scale protein assemblies on the PM, including cell adhesion structures and synapses, are likely made of archipelagoes of nanoliquid protein islands. Borrowing the concept of liquid–liquid phase separation to form micron-scale biocondensates, we propose that these nano-scale oligomers and assemblies are enabled by multiple weak but specific molecular interactions often involving intrinsically disordered regions. The signals from individual nanoliquid signaling complexes would occur as pulses. Single-molecule imaging emerges as a crucial technique for characterizing these transient nanoliquid assemblies on the PM, suggesting a shift toward a model where the fluidity of interactions underpins signal regulation and integration.

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来源期刊
Current Opinion in Cell Biology
Current Opinion in Cell Biology 生物-细胞生物学
CiteScore
14.60
自引率
1.30%
发文量
79
审稿时长
93 days
期刊介绍: Current Opinion in Cell Biology (COCEBI) is a highly respected journal that specializes in publishing authoritative, comprehensive, and systematic reviews in the field of cell biology. The journal's primary aim is to provide a clear and readable synthesis of the latest advances in cell biology, helping specialists stay current with the rapidly evolving field. Expert authors contribute to the journal by annotating and highlighting the most significant papers from the extensive body of research published annually, offering valuable insights and saving time for readers by distilling key findings. COCEBI is part of the Current Opinion and Research (CO+RE) suite of journals, which leverages the legacy of editorial excellence, high impact, and global reach to ensure that the journal is a widely read resource integral to scientists' workflow. It is published by Elsevier, a publisher known for its commitment to excellence in scientific publishing and the communication of reproducible biomedical research aimed at improving human health. The journal's content is designed to be an invaluable resource for a diverse audience, including researchers, lecturers, teachers, professionals, policymakers, and students.
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