秀丽隐杆线虫5S rRNA基因簇的遗传定位。

Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie Pub Date : 1986-08-01 DOI:10.1139/g86-080

D W Nelson, B M Honda

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引用次数: 2

摘要

我们在秀丽隐杆线虫的Bristol和Bergerac株中发现了一个限制性片段长度差异(rld)，它影响了5S rRNA基因簇两侧的基因组序列。我们利用这个rld作为分子标记，通过一系列的二因子和三因子株间杂交来跟踪5S rRNA基因簇的分离。结果表明，5S rRNA基因簇位于连锁群V右臂的unc-76和dpy-21之间，这一遗传定位为连锁群V的其他克隆序列原位杂交定位提供了“地标”。

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Genetic mapping of the 5S rRNA gene cluster of the nematode Caenorhabditis elegans.

We have identified a restriction fragment length difference (RFLD) affecting the genomic sequences immediately flanking the 5S rRNA gene cluster in the Bristol and Bergerac strains of the nematode Caenorhabditis elegans. We have used this RFLD as a molecular marker to follow the segregation of the 5S rRNA gene cluster through a series of two- and three-factor interstrain crosses. Our results show that the 5S rRNA gene cluster maps between unc-76 and dpy-21 on the right arm of linkage group V. This genetic localization provides a linkage group V "landmark" with which to localize other cloned sequences by in situ hybridization.

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Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie

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