中国新疆宫颈感染妇女的人类乳头瘤病毒 16 型 E4、E5 和 L2 基因变异分析。

IF 2.1 4区 医学 Q3 GENETICS & HEREDITY
Haozheng Cheng, Yangliu Dong, Le Wang, Xian Zhao, Xiangyi Zhe, Dongmei Li, Hongtao Li, Renfu Shao, Jing Tuo, Zemin Pan
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引用次数: 0

摘要

背景:新疆是宫颈癌的高发区:新疆是宫颈癌的高发区。方法:对90份HPV16感染阳性样本的HPV16基因组进行测序。方法:对90份HPV16感染阳性样本的HPV16基因组进行测序,分析E4、E5和L2基因的序列,以揭示新疆HPV16的序列变异以及变异在HPV16感染阳性样本中的分布:结果:在90份HPV16感染样本中,有81份样本的HPV16 E4基因出现变异,变异位点为18个核苷酸位点,其中8个位点为同义变异,11个位点为错义变异。90份HPV16感染样本的HPV16 E5和L2基因出现变异,其中E5基因有16个核苷酸变异位点(6个同义变异,11个错义变异),L2基因有100个核苷酸变异位点(37个同义变异,67个错义变异)。病例组中HPV16 L2基因错义变异G3377A、G3599A、G3703A和G3757A的频率高于对照组:系统发生树分析表明,87例样本为欧洲株,3例为亚洲株,无其他变异,G4181A与亚洲株有关。病例组中HPV16 L2基因错义变异G3377A、G3599A、G3703A和G3757A的发生率明显高于对照组。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Analysis of human papillomavirus type 16 E4, E5 and L2 gene variations among women with cervical infection in Xinjiang, China.

Background: There is a high incidence of cervical cancer in Xinjiang. Genetic variation in human papillomavirus may increase its ability to invade, spread, and escape host immune response.

Methods: HPV16 genome was sequenced for 90 positive samples of HPV16 infection. Sequences of the E4, E5 and L2 genes were analysed to reveal sequence variation of HPV16 in Xinjiang and the distribution of variation among the positive samples of HPV16 infection.

Results: Eighty-one of the 90 samples of HPV16 infection showed variation in HPV16 E4 gene with 18 nucleotide variation sites, of which 8 sites were synonymous variations and 11 missense variations. 90 samples of HPV16 infection showed variation in HPV16 E5 and L2 genes with 16 nucleotide variation sites (6 synonymous, 11 missense variations) in the E5 gene and 100 nucleotide variation sites in L2 gene (37 synonymous, 67 missense variations). The frequency of HPV16 L2 gene missense variations G3377A, G3599A, G3703A, and G3757A was higher in the case groups than in the control groups.

Conclusions: Phylogenetic tree analysis showed that 87 samples were European strains, 3 cases were Asian strains, there were no other variations, and G4181A was related to Asian strains. HPV16 L2 gene missense variations G3377A, G3599A, G3703A, and G3757A were significantly more frequent in the case groups than in the control groups.

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来源期刊
BMC Medical Genomics
BMC Medical Genomics 医学-遗传学
CiteScore
3.90
自引率
0.00%
发文量
243
审稿时长
3.5 months
期刊介绍: BMC Medical Genomics is an open access journal publishing original peer-reviewed research articles in all aspects of functional genomics, genome structure, genome-scale population genetics, epigenomics, proteomics, systems analysis, and pharmacogenomics in relation to human health and disease.
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