研究大麦嫩叶的体外生物活性与其生物活性化合物的相关性。

IF 1.8 4区 生物学 Q3 BIOLOGY
Ferenc Lantos, Vivien Váczi, Ingrid Gyalai, Tímea Süli-Zakar, Adrienn Szarvas, Melinda Tar, Péter Püski, Ákos Bajtel, Tivadar Kiss
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引用次数: 0

摘要

谷物嫩芽被广泛视为超级食品,其潜在的抗氧化活性和抗氧化相关效应(如抗癌)对健康有益。本研究旨在检测冬虫夏草甲醇提取物和水提取物的化学特征,并使用 DDPH 和 ORAC 评估其抗氧化活性。此外,还对黄嘌呤氧化酶的抑制作用进行了筛选。采用 TLC 生物自动层析技术确定了提取物中存在的化合物的极性,这些化合物具有最强的自由基清除活性。甲醇提取物和水提取物中的总黄酮含量分别为 0.14 毫克 QE/g 和 0.012 毫克 QE/g。甲醇提取物的抗氧化活性为 0.97 ± 0.13 mmol TE/g,比没有活性的水提取物更强。本研究提出了关于 H. vulgare 的黄嘌呤抑制活性的新发现。甲醇提取物对黄嘌呤氧化酶有中度抑制作用,抑制率为 23.24%。我们将研究结果与 Triticum aestivum 的植物化学和药理分析进行了比较,并进一步与文献报道的数据进行了比较。我们观察到 H. vulgare 的化学和药理特性并不一致,这可能是由于使用了在不同无性期收获的草药材料和不同的提取方法造成的。我们的研究结果表明,收获的时间和提取方法可能对获得 H. vulgare 的最佳植物化学成分,从而提高其药理活性起着至关重要的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Investigation of in vitro biological activity of young Hordeum vulgare leaf in correlation with its bioactive compounds.

Young shoots of cereals are widely regarded as superfoods with health benefits attributed to their potential antioxidant activity and antioxidant-related effects (e.g. anticancer). The current study aimed to examine the chemical characteristics of Hordeum vulgare methanolic and aqueous extracts and assess their antioxidant activity using the DDPH and ORAC. Furthermore, the inhibitory effect of xanthine oxidase was screened. TLC bioautography was employed to determine the polarity of the compounds present in the extracts that exhibited the most potent free radical scavenging activity. Total flavonoid content of the methanolic and aqueous extracts was 0.14 mg QE/g and 0.012 mg QE/g, respectively. The antioxidant activity of the methanolic extract was found to be more potent, with a value of 0.97 ± 0.13 mmol TE/g than the aqueous extract which had no activity. This study presents novel findings on the xanthine inhibitory activity of H. vulgare. The methanolic extract demonstrated moderate inhibition of xanthine oxidase with a value of 23.24%. The results of our study were compared with the phytochemical and pharmacological analysis of Triticum aestivum, and further comparison was made with the data reported in the literature. Inconsistencies were observed in the chemical and pharmacological properties of H. vulgare, which could be a result of using herbal material harvested in different vegetative phases and various methods used for extraction. The findings of our study indicate that the timing of the harvest and extraction method may play crucial role in attaining the optimal phytochemical composition of H. vulgare, hence enhancing its pharmacological activity.

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来源期刊
Biologia futura
Biologia futura Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)
CiteScore
3.50
自引率
0.00%
发文量
27
期刊介绍: How can the scientific knowledge we possess now influence that future? That is, the FUTURE of Earth and life − of humankind. Can we make choices in the present to change our future? How can 21st century biological research ask proper scientific questions and find solid answers? Addressing these questions is the main goal of Biologia Futura (formerly Acta Biologica Hungarica). In keeping with the name, the new mission is to focus on areas of biology where major advances are to be expected, areas of biology with strong inter-disciplinary connection and to provide new avenues for future research in biology. Biologia Futura aims to publish articles from all fields of biology.
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