外周生物钟紊乱可能是睡眠不足引起的昼夜脂质代谢失调的原因之一。

IF 3.9 2区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Chufan Zhou , Ziping Hu , Xuan Liu , Yuefan Wang , Shougang Wei , Zhifeng Liu
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引用次数: 0

摘要

研究目的本研究旨在探讨睡眠剥夺(SD)对肝脏和白色脂肪组织(WAT)在明暗阶段的脂质代谢或脂质代谢调节的影响,并探索SD对脂质代谢的昼夜效应与时钟基因相关的可能机制:方法:对2个月大的雄性C57BL/6J小鼠连续10天每天剥夺睡眠20小时,并进行弱强迫运动。记录SD小鼠和对照组小鼠的体重和食物消耗量,然后在ZT(Zeitgeber时间)2和ZT 14时将小鼠处死。对小鼠的外周时钟基因、参与脂肪合成和分解的酶以及肝脏中褪黑激素信号通路介导的脂质代谢进行了评估。利用非靶向代谢组学和串联质量标签(TMT)蛋白质组学确定肝脏中不同的脂质代谢途径:结果:SD后体重增加和每日食物消耗量显著增加。观察到肝脏外周时钟的昼夜调节以及参与脂肪合成和分解代谢的酶在WAT中发生了严重破坏,其中肝脏脂质代谢通路受到了极大影响,而褪黑激素信号通路介导的脂质代谢则表现出中等程度的变化:结论:连续十天的 SD 会增加小鼠的体重增加和每日食物消耗量。结论:连续十天的自毁增加了小鼠的体重增加和每日食物消耗量,此外,自毁还严重破坏了小鼠脂肪乳和肝脏在光照和黑暗期间的脂质代谢。这些昼夜变化可能与外周生物钟紊乱有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Disruption of the peripheral biological clock may play a role in sleep deprivation-induced dysregulation of lipid metabolism in both the daytime and nighttime phases

Disruption of the peripheral biological clock may play a role in sleep deprivation-induced dysregulation of lipid metabolism in both the daytime and nighttime phases

Study objectives

This study aimed to examine the effect of sleep deprivation (SD) on lipid metabolism or lipid metabolism regulation in the liver and white adipose tissue (WAT) during the light and dark phases and explored the possible mechanisms underlying the diurnal effect of SD on lipid metabolism associated with clock genes.

Methods

Male C57BL/6J mice aged 2 months were deprived of sleep daily for 20 h for ten consecutive days with weakly forced locomotion. The body weights and food consumption levels of the SD and control mice were recorded, and the mice were then sacrificed at ZT (zeitgeber time) 2 and ZT 14. The peripheral clock genes, enzymes involved in fat synthesis and catabolism in the WAT, and melatonin signalling pathway-mediated lipid metabolism in the liver were assessed. Untargeted metabolomics and tandem mass tag (TMT) proteomics were used to identify differential lipid metabolism pathways in the liver.

Results

Bodyweight gain and daily food consumption were dramatically elevated after SD. Profound disruptions in the diurnal regulation of the hepatic peripheral clock and enzymes involved in fat synthesis and catabolism in the WAT were observed, with a strong emphasis on hepatic lipid metabolic pathways, while melatonin signalling pathway-mediated lipid metabolism exhibited moderate changes.

Conclusions

In mice, ten consecutive days of SD increased body weight gain and daily food consumption. In addition, SD profoundly disrupted lipid metabolism in the WAT and liver during the light and dark periods. These diurnal changes may be related to disorders of the peripheral biological clock.

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来源期刊
CiteScore
11.00
自引率
2.10%
发文量
109
审稿时长
53 days
期刊介绍: BBA Molecular and Cell Biology of Lipids publishes papers on original research dealing with novel aspects of molecular genetics related to the lipidome, the biosynthesis of lipids, the role of lipids in cells and whole organisms, the regulation of lipid metabolism and function, and lipidomics in all organisms. Manuscripts should significantly advance the understanding of the molecular mechanisms underlying biological processes in which lipids are involved. Papers detailing novel methodology must report significant biochemical, molecular, or functional insight in the area of lipids.
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