{"title":"模仿巨噬细胞聚集体的细胞膜工程多肽纳米网,用于增强抗菌治疗。","authors":"Jiang Xiao, Zhongquan Song, Tengfei Liu, Zengchao Guo, Xiaohui Liu, Hui Jiang, Xuemei Wang","doi":"10.1002/smll.202401845","DOIUrl":null,"url":null,"abstract":"<p><p>Drug-resistant bacterial infections and their lipopolysaccharide-related inflammatory complications continue to pose significant challenges in traditional treatments. Inspired by the rapid initiation of resident macrophages to form aggregates for efficient antibacterial action, this study proposes a multifunctional and enhanced antibacterial strategy through the construction of novel biomimetic cell membrane polypeptide nanonets (R-DPB-TA-Ce). The design involves the fusion of end-terminal lipidated polypeptides containing side-chain cationic boronic acid groups (DNPLBA) with cell membrane intercalation engineering (R-DPB), followed by coordination with the tannic acid-cerium complex (TA-Ce) to assemble into a biomimetic nanonet through boronic acid-polyphenol-metal ion interactions. In addition to the ability of RAW 264.7 macrophages cell membrane components' (R) ability to neutralize lipopolysaccharide (LPS), R-DPB-TA-Ce demonstrated enhanced capture of bacteria and its LPS, leveraging nanoconfinement-enhanced multiple interactions based on the boronic acid-polyphenol nanonets skeleton combined with polysaccharide. Utilizing these advantages, indocyanine green (ICG) is further employed as a model drug for delivery, showcasing the exceptional treatment effect of R-DPB-TA-Ce as a new biomimetic assembled drug delivery system in antibacterial, anti-inflammatory, and wound healing promotion. 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引用次数: 0
摘要
耐药性细菌感染及其与脂多糖相关的炎症并发症仍然是传统治疗方法面临的重大挑战。受驻留巨噬细胞迅速形成聚集体以达到高效抗菌作用的启发,本研究通过构建新型仿生物细胞膜多肽纳米网(R-DPB-TA-Ce),提出了一种多功能增强型抗菌策略。该设计包括将含有侧链阳离子硼酸基团(DNPLBA)的末端脂化多肽与细胞膜插层工程(R-DPB)融合,然后与单宁酸铈复合物(TA-Ce)配位,通过硼酸-多酚-金属离子的相互作用组装成仿生纳米网。除了 RAW 264.7 巨噬细胞细胞膜成分(R)中和脂多糖(LPS)的能力外,R-DPB-TA-Ce 还利用基于硼酸-多酚纳米网骨架与多糖结合的纳米膦强化多重相互作用,增强了对细菌及其 LPS 的捕获能力。利用这些优势,吲哚菁绿(ICG)被进一步用作给药模型,展示了 R-DPB-TA-Ce 作为一种新型仿生组装给药系统在抗菌、消炎和促进伤口愈合方面的卓越治疗效果。因此,这种模拟巨噬细胞聚集体的策略有望进一步应用于各类细胞膜工程,以增强抗菌治疗效果。
Drug-resistant bacterial infections and their lipopolysaccharide-related inflammatory complications continue to pose significant challenges in traditional treatments. Inspired by the rapid initiation of resident macrophages to form aggregates for efficient antibacterial action, this study proposes a multifunctional and enhanced antibacterial strategy through the construction of novel biomimetic cell membrane polypeptide nanonets (R-DPB-TA-Ce). The design involves the fusion of end-terminal lipidated polypeptides containing side-chain cationic boronic acid groups (DNPLBA) with cell membrane intercalation engineering (R-DPB), followed by coordination with the tannic acid-cerium complex (TA-Ce) to assemble into a biomimetic nanonet through boronic acid-polyphenol-metal ion interactions. In addition to the ability of RAW 264.7 macrophages cell membrane components' (R) ability to neutralize lipopolysaccharide (LPS), R-DPB-TA-Ce demonstrated enhanced capture of bacteria and its LPS, leveraging nanoconfinement-enhanced multiple interactions based on the boronic acid-polyphenol nanonets skeleton combined with polysaccharide. Utilizing these advantages, indocyanine green (ICG) is further employed as a model drug for delivery, showcasing the exceptional treatment effect of R-DPB-TA-Ce as a new biomimetic assembled drug delivery system in antibacterial, anti-inflammatory, and wound healing promotion. Thus, this strategy of mimicking macrophage aggregates is anticipated to be further applicable to various types of cell membrane engineering for enhanced antibacterial treatment.
期刊介绍:
Small serves as an exceptional platform for both experimental and theoretical studies in fundamental and applied interdisciplinary research at the nano- and microscale. The journal offers a compelling mix of peer-reviewed Research Articles, Reviews, Perspectives, and Comments.
With a remarkable 2022 Journal Impact Factor of 13.3 (Journal Citation Reports from Clarivate Analytics, 2023), Small remains among the top multidisciplinary journals, covering a wide range of topics at the interface of materials science, chemistry, physics, engineering, medicine, and biology.
Small's readership includes biochemists, biologists, biomedical scientists, chemists, engineers, information technologists, materials scientists, physicists, and theoreticians alike.