Pu Feng, Jun Liu, Lian-Jun Bao, Eddy Y Zeng, Chunfeng Ma, Lingling Wang, Guangzhao Zhang and Xiangjun Gong*,
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引用次数: 0
摘要
利用低振幅电脉冲（EP）进行海洋生物防污是一种节能环保的方法，但防止生物污染的潜在机制仍不清楚。在本研究中，研究人员考察了模型微生物--铜绿假单胞菌（PAO1）--在低幅阴极电脉冲下的三维粘附动态，并将其作为施加电压及其持续时间（td）的函数。结果表明，即使去除极压，附着的细菌也会从电极表面逃逸。逸出细菌比率、逸出诱导期和脱离持续时间受 EP 振幅的影响很大，但在 td ≥ 5 分钟时受 td 的影响较小。从表面逃逸的 PAO1 的加速度表明，它们的鞭毛运动是由 EP 驱动的。特别是，EP 使游动的细菌能够在 rsmA 基因的调控下进行可持续的适应性运动。因此，它们在表面附近的积聚较少。在低振幅 EP 的作用下，粘附细菌的推进力和游动细菌的自适应逃逸能力都得到了增强。因此，低振幅和短持续时间的 EP 在可持续抗生物污染应用中大有可为。
Adaptive Escape of Pseudomonas aeruginosa by Application of Low-Amplitude Electric Pulses
Marine antibiofouling using low-amplitude electric pulses (EP) is an energy-efficient and eco-friendly approach, but potential mechanisms for preventing biofouling remain unclear. In the present study, the 3D adhesion dynamics of a model microorganism─Pseudomonas aeruginosa (PAO1)─under low-amplitude cathodic EP were examined as a function of applying voltage and its duration (td). The results demonstrated that adhered bacteria escaped from the electrode surface even when EP was removed. The escaped bacteria ratio, induction period of escape, and duration of the detachment were influenced profoundly by EP amplitude but slightly by td when td ≥ 5 min. The acceleration of escaped PAO1 from the surface indicated that their flagellar motor was powered by EP. Particularly, EP enabled swimming bacteria to have adaptive motions that were sustainable and regulated by the gene rsmA. As a result, they had less accumulation near the surface. The propulsion of adhered bacteria and adaptive escape of swimming bacteria were enhanced in response to low-amplitude EP. Hence, low-amplitude and short-duration EP is promising for sustainable antibiofouling applications.
期刊介绍：
Langmuir is an interdisciplinary journal publishing articles in the following subject categories:
Colloids: surfactants and self-assembly, dispersions, emulsions, foams
Interfaces: adsorption, reactions, films, forces
Biological Interfaces: biocolloids, biomolecular and biomimetic materials
Materials: nano- and mesostructured materials, polymers, gels, liquid crystals
Electrochemistry: interfacial charge transfer, charge transport, electrocatalysis, electrokinetic phenomena, bioelectrochemistry
Devices and Applications: sensors, fluidics, patterning, catalysis, photonic crystals
However, when high-impact, original work is submitted that does not fit within the above categories, decisions to accept or decline such papers will be based on one criteria: What Would Irving Do?
Langmuir ranks #2 in citations out of 136 journals in the category of Physical Chemistry with 113,157 total citations. The journal received an Impact Factor of 4.384*.
This journal is also indexed in the categories of Materials Science (ranked #1) and Multidisciplinary Chemistry (ranked #5).
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