胶质细胞系源性神经营养因子和基质对青春期前领猯睾丸组织体外培养的协同作用

IF 3.3 3区 生物学 Q3 CELL BIOLOGY
Andreia Maria da Silva, Ana Glória Pereira, Luana Graziele Pereira Bezerra, Andreza Vieira Brasil, Alexsandra Fernandes Pereira, Moacir Franco de Oliveira, Ana Paula Ribeiro Rodrigues, Lucy Vanessa Sulca Ñaupas, Pierre Comizzoli, Alexandre Rodrigues Silva
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引用次数: 0

摘要

我们评估了不同培养基和不同浓度的胶质细胞系源性神经营养因子(GDNF)对青春期前领啄木鸟睾丸组织体外培养(IVC)的影响。采集 5 只啄木鸟的睾丸,将其破碎并培养 28 天(34°C 和 5% CO2)。培养基为杜氏改良基本培养基(DMEM)或无干细胞血清培养基(StemPro-34™ SFM),两者均添加不同浓度的GDNF(0、10或20纳克/毫升)。片段培养在0.75%琼脂糖凝胶的平面上,每7天评估一次片段面积、组织形态学、细胞活力和增殖活性。数据以平均值±标准误差表示,并通过 Kruskal-Wallis's 和 Tukey 检验进行分析。片段面积在 28 天的培养过程中有所减少,与处理方法无关。在形态学方面,StemPro-37 SFM 培养基加 10 ng/mL GDNF 与使用任何 GDNF 浓度的 DMEM 相比,在所有时间点的得分都更高(p <.05)。28 天后,在所有处理中观察到了相似的细胞活力(约 70%)(p > .05)。在增殖细胞核抗原检测中,只有 DMEM 加 10 ng/mL GDNF 在第 14 天和第 28 天改善了细胞增殖(p < .05)。观察嗜杏仁核组织区域,28 天后,精原细胞和 Sertoli 细胞的细胞增殖能力在不同处理之间没有差异(p >.05)。总之,DMEM 和 StemPro-34 SFM 是用于青春期前山雀睾丸组织 IVC 的适当培养基。补充 GDNF,尤其是 10 ng/mL 浓度的 GDNF,似乎对维持细胞存活和增殖至关重要。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Synergistic effects of glial cell line-derived neurotrophic factor and base-medium on in vitro culture of testicular tissue derived from prepubertal collared peccary

We evaluated the influence of different media plus various concentrations of Glial cell line-derived neurotrophic factor (GDNF) during the in vitro culture (IVC) of testicular tissues from prepubertal collared peccary. Testes from 5 individuals were collected, fragmented and cultured for 28 days (34°C and 5% CO2). Culture media were Dulbecco's modified essential medium (DMEM) or stem cell serum free media (StemPro-34™ SFM), both supplemented with various concentrations of GDNF (0, 10, or 20 ng/mL). Fragments were cultured on the flat surface of 0.75% agarose gel and were evaluated every 7 days for fragment area, histomorphology, cellular viability, and proliferative activity. Data were expressed as mean ± standard error and analyzed by Kruskal–Wallis's and Tukey test. Fragments area decreased over the 28 days-culture, regardless of the treatment. For morphology, the StemPro-37 SFM medium plus 10 ng/mL GDNF provided higher scores at all time points in comparison to DMEM using any GDNF concentration (p < .05). After 28 days, similar cellular viability (~70%) was observed in all treatments (p > .05). For proliferating cell nuclear antigen assay, only DMEM plus 10 ng/mL GDNF improved (p < .05) cellular proliferation on Days 14 and 28. Looking at argyrophilic nucleolar organizing regions, after 28 days, there were no differences among treatments regarding cell proliferative capacity for both spermatogonia and Sertoli cells (p > .05). In summary, the DMEM and StemPro-34 SFM are adequate medium for IVC of prepubertal peccary testicular tissue. Supplementation with GDNF, especially at a 10 ng/mL concentration, appears to be essential for the maintenance of cell survival and proliferation.

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来源期刊
Cell Biology International
Cell Biology International 生物-细胞生物学
CiteScore
7.60
自引率
0.00%
发文量
208
审稿时长
1 months
期刊介绍: Each month, the journal publishes easy-to-assimilate, up-to-the minute reports of experimental findings by researchers using a wide range of the latest techniques. Promoting the aims of cell biologists worldwide, papers reporting on structure and function - especially where they relate to the physiology of the whole cell - are strongly encouraged. Molecular biology is welcome, as long as articles report findings that are seen in the wider context of cell biology. In covering all areas of the cell, the journal is both appealing and accessible to a broad audience. Authors whose papers do not appeal to cell biologists in general because their topic is too specialized (e.g. infectious microbes, protozoology) are recommended to send them to more relevant journals. Papers reporting whole animal studies or work more suited to a medical journal, e.g. histopathological studies or clinical immunology, are unlikely to be accepted, unless they are fully focused on some important cellular aspect. These last remarks extend particularly to papers on cancer. Unless firmly based on some deeper cellular or molecular biological principle, papers that are highly specialized in this field, with limited appeal to cell biologists at large, should be directed towards journals devoted to cancer, there being very many from which to choose.
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