{"title":"探索生物相容性抗坏血酸还原和稳定金纳米粒子,作为溶液中银离子的灵敏和选择性检测纳米平台","authors":"Titilope John Jayeoye, Sudarshan Singh, Fredrick Nwude Eze, Opeyemi Joshua Olatunji, Oladipupo Odunayo Olatunde, Omaka Ndukaku Omaka, Oghale Beauty Odogiyon, Kingsley Ezechukwu Okpara","doi":"10.1007/s11468-024-02413-2","DOIUrl":null,"url":null,"abstract":"<p>Silver ion (Ag<sup>+</sup>) is one of the heavy metals (HMs) of interest that must be regularly profiled, by virtue of its toxicity and eco-physiological implications. Herein, a colorimetric assay for sensitive detection of Ag<sup>+</sup> ion in solution is developed. To avoid the introduction of toxic chemicals in the material synthesis stage, ascorbic acid (AA) was employed as the reducing/stabilizing agent at room temperature (RT), for the gold nanoparticle (AuNPs) synthesis. The biocompatibility of the synthesized AA-AuNPs was demonstrated by the cytotoxicity test using MTT assay on mouse macrophage cells (RAW 264.7), which revealed that AA-AuNPs imparted no destruction on the tested cells. Further, AA capping on the AuNP surfaces was confirmed by Raman and Fourier transform infrared spectroscopy (FTIR). At the optimal detection conditions, the addition of Ag<sup>+</sup> to AA-AuNPs solution (pH 10) resulted in naked-eye color transitions from red to orange and yellow, with a blue shift in the absorption maximum from 522 to 400 nm. This is attributed to the reduction of Ag<sup>+</sup> on the initially synthesized AA-AuNPs probe, induced by the capping agent, forming Au@Ag core–shell nanomaterials. The analytic response (<i>A</i><sub>f</sub>-<i>A</i><sub>0</sub>)400 nm plotted against Ag<sup>+</sup> concentrations was linear within 0.05–12.50 and 12.50–150.00 µM, with estimated limit of detection (LOD) of 15.8 nM. For practical usage, the probe was deployed for Ag<sup>+</sup> detection in lake water sample, showing impressive accuracy (95.5–104.7%) and precision.</p>","PeriodicalId":736,"journal":{"name":"Plasmonics","volume":null,"pages":null},"PeriodicalIF":3.3000,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Exploration of Biocompatible Ascorbic Acid Reduced and Stabilized Gold Nanoparticles, as Sensitive and Selective Detection Nanoplatform for Silver Ion in Solution\",\"authors\":\"Titilope John Jayeoye, Sudarshan Singh, Fredrick Nwude Eze, Opeyemi Joshua Olatunji, Oladipupo Odunayo Olatunde, Omaka Ndukaku Omaka, Oghale Beauty Odogiyon, Kingsley Ezechukwu Okpara\",\"doi\":\"10.1007/s11468-024-02413-2\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Silver ion (Ag<sup>+</sup>) is one of the heavy metals (HMs) of interest that must be regularly profiled, by virtue of its toxicity and eco-physiological implications. Herein, a colorimetric assay for sensitive detection of Ag<sup>+</sup> ion in solution is developed. To avoid the introduction of toxic chemicals in the material synthesis stage, ascorbic acid (AA) was employed as the reducing/stabilizing agent at room temperature (RT), for the gold nanoparticle (AuNPs) synthesis. The biocompatibility of the synthesized AA-AuNPs was demonstrated by the cytotoxicity test using MTT assay on mouse macrophage cells (RAW 264.7), which revealed that AA-AuNPs imparted no destruction on the tested cells. Further, AA capping on the AuNP surfaces was confirmed by Raman and Fourier transform infrared spectroscopy (FTIR). At the optimal detection conditions, the addition of Ag<sup>+</sup> to AA-AuNPs solution (pH 10) resulted in naked-eye color transitions from red to orange and yellow, with a blue shift in the absorption maximum from 522 to 400 nm. This is attributed to the reduction of Ag<sup>+</sup> on the initially synthesized AA-AuNPs probe, induced by the capping agent, forming Au@Ag core–shell nanomaterials. The analytic response (<i>A</i><sub>f</sub>-<i>A</i><sub>0</sub>)400 nm plotted against Ag<sup>+</sup> concentrations was linear within 0.05–12.50 and 12.50–150.00 µM, with estimated limit of detection (LOD) of 15.8 nM. For practical usage, the probe was deployed for Ag<sup>+</sup> detection in lake water sample, showing impressive accuracy (95.5–104.7%) and precision.</p>\",\"PeriodicalId\":736,\"journal\":{\"name\":\"Plasmonics\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.3000,\"publicationDate\":\"2024-07-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plasmonics\",\"FirstCategoryId\":\"101\",\"ListUrlMain\":\"https://doi.org/10.1007/s11468-024-02413-2\",\"RegionNum\":4,\"RegionCategory\":\"物理与天体物理\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, PHYSICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plasmonics","FirstCategoryId":"101","ListUrlMain":"https://doi.org/10.1007/s11468-024-02413-2","RegionNum":4,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, PHYSICAL","Score":null,"Total":0}
Exploration of Biocompatible Ascorbic Acid Reduced and Stabilized Gold Nanoparticles, as Sensitive and Selective Detection Nanoplatform for Silver Ion in Solution
Silver ion (Ag+) is one of the heavy metals (HMs) of interest that must be regularly profiled, by virtue of its toxicity and eco-physiological implications. Herein, a colorimetric assay for sensitive detection of Ag+ ion in solution is developed. To avoid the introduction of toxic chemicals in the material synthesis stage, ascorbic acid (AA) was employed as the reducing/stabilizing agent at room temperature (RT), for the gold nanoparticle (AuNPs) synthesis. The biocompatibility of the synthesized AA-AuNPs was demonstrated by the cytotoxicity test using MTT assay on mouse macrophage cells (RAW 264.7), which revealed that AA-AuNPs imparted no destruction on the tested cells. Further, AA capping on the AuNP surfaces was confirmed by Raman and Fourier transform infrared spectroscopy (FTIR). At the optimal detection conditions, the addition of Ag+ to AA-AuNPs solution (pH 10) resulted in naked-eye color transitions from red to orange and yellow, with a blue shift in the absorption maximum from 522 to 400 nm. This is attributed to the reduction of Ag+ on the initially synthesized AA-AuNPs probe, induced by the capping agent, forming Au@Ag core–shell nanomaterials. The analytic response (Af-A0)400 nm plotted against Ag+ concentrations was linear within 0.05–12.50 and 12.50–150.00 µM, with estimated limit of detection (LOD) of 15.8 nM. For practical usage, the probe was deployed for Ag+ detection in lake water sample, showing impressive accuracy (95.5–104.7%) and precision.
期刊介绍:
Plasmonics is an international forum for the publication of peer-reviewed leading-edge original articles that both advance and report our knowledge base and practice of the interactions of free-metal electrons, Plasmons.
Topics covered include notable advances in the theory, Physics, and applications of surface plasmons in metals, to the rapidly emerging areas of nanotechnology, biophotonics, sensing, biochemistry and medicine. Topics, including the theory, synthesis and optical properties of noble metal nanostructures, patterned surfaces or materials, continuous or grated surfaces, devices, or wires for their multifarious applications are particularly welcome. Typical applications might include but are not limited to, surface enhanced spectroscopic properties, such as Raman scattering or fluorescence, as well developments in techniques such as surface plasmon resonance and near-field scanning optical microscopy.