一种具有独特 Gal_Lectin 和 CUB 结构域的新型凝集素可介导巨牡蛎的血细胞吞噬作用

IF 2.7 3区 农林科学 Q1 FISHERIES
Wenwen Yang , Jiejie Sun , Jinyuan Leng , Yinan Li , Qiuyan Guo , Lingling Wang , Linsheng Song
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引用次数: 0

摘要

无脊椎动物凝集素具有结构多样性,在先天性免疫反应中发挥着识别和清除病原体的关键作用。本研究从太平洋牡蛎(Crassostrea gigas)中鉴定出一种新型凝集素,它含有一个Gal_Lectin、一个CUB和一个跨膜结构域(定义为CgGal-CUB)。CgGal-CUB mRNA 可在所有受检组织中检测到,其中内收肌中的表达量最高(是血细胞中表达量的 11.00 倍,p < 0.05)。在绚丽弧菌刺激后的 3、24、48 和 72 h,血细胞中 CgGal-CUB mRNA 的表达水平显著上调(分别是对照组的 8.37 倍、12.13 倍、4.28 倍和 10.14 倍)。重组 CgGal-CUB(rCgGal-CUB)具有与甘露糖(MAN)、肽聚糖(PGN)、D-(+)-半乳糖和一水鼠李糖以及革兰氏阴性菌(大肠杆菌、白弧菌和弧菌)结合的能力。rCgGal-CUB 还能凝集白芨藻,抑制白芨藻的生长。此外,rCgGal-CUB 还能增强血细胞对白芨的吞噬作用,在 CgGal-CUB 抗体的阻断实验中,血细胞的吞噬率有所下降。这些结果表明,CgGal-CUB可作为一种模式识别受体与各种PAMPs和细菌结合,增强血细胞对白芨的吞噬作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A novel lectin with a distinct Gal_Lectin and CUB domain mediates haemocyte phagocytosis in oyster Crassostrea gigas

Invertebrate lectins exhibit structural diversity and play crucial roles in the innate immune responses by recognizing and eliminating pathogens. In the present study, a novel lectin containing a Gal_Lectin, a CUB and a transmembrane domain was identified from the Pacific oyster Crassostrea gigas (defined as CgGal-CUB). CgGal-CUB mRNA was detectable in all the examined tissues with the highest expression in adductor muscle (11.00-fold of that in haemocytes, p < 0.05). The expression level of CgGal-CUB mRNA in haemocytes was significantly up-regulated at 3, 24, 48 and 72 h (8.37-fold, 12.13-fold, 4.28-fold and 10.14-fold of that in the control group, respectively) after Vibrio splendidus stimulation. The recombinant CgGal-CUB (rCgGal-CUB) displayed binding capability to Mannan (MAN), peptidoglycan (PGN), D-(+)-Galactose and L-Rhamnose monohydrate, as well as Gram-negative bacteria (Escherichia coli, V. splendidus and Vibrio anguillarum), Gram-positive bacteria (Micrococcus luteus, Staphylococcus aureus, and Bacillus sybtilis) and fungus (Pichia pastoris). rCgGal-CUB was also able to agglutinate V. splendidus, and inhibit V. splendidus growth. Furthermore, rCgGal-CUB exhibited the activities of enhancing the haemocyte phagocytosis towards V. splendidus, and the phagocytosis rate of haemocytes was descended in blockage assay with CgGal-CUB antibody. These results suggested that CgGal-CUB served as a pattern recognition receptor to bind various PAMPs and bacteria, and enhanced the haemocyte phagocytosis towards V. splendidus.

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来源期刊
CiteScore
6.20
自引率
6.90%
发文量
206
审稿时长
49 days
期刊介绍: Developmental and Comparative Immunology (DCI) is an international journal that publishes articles describing original research in all areas of immunology, including comparative aspects of immunity and the evolution and development of the immune system. Manuscripts describing studies of immune systems in both vertebrates and invertebrates are welcome. All levels of immunological investigations are appropriate: organismal, cellular, biochemical and molecular genetics, extending to such fields as aging of the immune system, interaction between the immune and neuroendocrine system and intestinal immunity.
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