Wenwen Yang , Jiejie Sun , Jinyuan Leng , Yinan Li , Qiuyan Guo , Lingling Wang , Linsheng Song
{"title":"一种具有独特 Gal_Lectin 和 CUB 结构域的新型凝集素可介导巨牡蛎的血细胞吞噬作用","authors":"Wenwen Yang , Jiejie Sun , Jinyuan Leng , Yinan Li , Qiuyan Guo , Lingling Wang , Linsheng Song","doi":"10.1016/j.dci.2024.105222","DOIUrl":null,"url":null,"abstract":"<div><p>Invertebrate lectins exhibit structural diversity and play crucial roles in the innate immune responses by recognizing and eliminating pathogens. In the present study, a novel lectin containing a Gal_Lectin, a CUB and a transmembrane domain was identified from the Pacific oyster <em>Crassostrea gigas</em> (defined as <em>Cg</em>Gal-CUB). <em>Cg</em>Gal-CUB mRNA was detectable in all the examined tissues with the highest expression in adductor muscle (11.00-fold of that in haemocytes, <em>p</em> < 0.05). The expression level of <em>Cg</em>Gal-CUB mRNA in haemocytes was significantly up-regulated at 3, 24, 48 and 72 h (8.37-fold, 12.13-fold, 4.28-fold and 10.14-fold of that in the control group, respectively) after <em>Vibrio splendidus</em> stimulation. The recombinant <em>Cg</em>Gal-CUB (r<em>Cg</em>Gal-CUB) displayed binding capability to Mannan (MAN), peptidoglycan (PGN), D-(+)-Galactose and L-Rhamnose monohydrate, as well as Gram-negative bacteria (<em>Escherichia coli</em>, <em>V. splendidus</em> and <em>Vibrio anguillarum</em>), Gram-positive bacteria (<em>Micrococcus luteus</em>, <em>Staphylococcus aureus</em>, and <em>Bacillus sybtilis</em>) and fungus (<em>Pichia pastoris</em>). r<em>Cg</em>Gal-CUB was also able to agglutinate <em>V. splendidus</em>, and inhibit <em>V. splendidus</em> growth. Furthermore, r<em>Cg</em>Gal-CUB exhibited the activities of enhancing the haemocyte phagocytosis towards <em>V. splendidus</em>, and the phagocytosis rate of haemocytes was descended in blockage assay with <em>C</em>gGal-CUB antibody. These results suggested that <em>Cg</em>Gal-CUB served as a pattern recognition receptor to bind various PAMPs and bacteria, and enhanced the haemocyte phagocytosis towards <em>V. splendidus</em>.</p></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"159 ","pages":"Article 105222"},"PeriodicalIF":2.7000,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A novel lectin with a distinct Gal_Lectin and CUB domain mediates haemocyte phagocytosis in oyster Crassostrea gigas\",\"authors\":\"Wenwen Yang , Jiejie Sun , Jinyuan Leng , Yinan Li , Qiuyan Guo , Lingling Wang , Linsheng Song\",\"doi\":\"10.1016/j.dci.2024.105222\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Invertebrate lectins exhibit structural diversity and play crucial roles in the innate immune responses by recognizing and eliminating pathogens. In the present study, a novel lectin containing a Gal_Lectin, a CUB and a transmembrane domain was identified from the Pacific oyster <em>Crassostrea gigas</em> (defined as <em>Cg</em>Gal-CUB). <em>Cg</em>Gal-CUB mRNA was detectable in all the examined tissues with the highest expression in adductor muscle (11.00-fold of that in haemocytes, <em>p</em> < 0.05). The expression level of <em>Cg</em>Gal-CUB mRNA in haemocytes was significantly up-regulated at 3, 24, 48 and 72 h (8.37-fold, 12.13-fold, 4.28-fold and 10.14-fold of that in the control group, respectively) after <em>Vibrio splendidus</em> stimulation. The recombinant <em>Cg</em>Gal-CUB (r<em>Cg</em>Gal-CUB) displayed binding capability to Mannan (MAN), peptidoglycan (PGN), D-(+)-Galactose and L-Rhamnose monohydrate, as well as Gram-negative bacteria (<em>Escherichia coli</em>, <em>V. splendidus</em> and <em>Vibrio anguillarum</em>), Gram-positive bacteria (<em>Micrococcus luteus</em>, <em>Staphylococcus aureus</em>, and <em>Bacillus sybtilis</em>) and fungus (<em>Pichia pastoris</em>). r<em>Cg</em>Gal-CUB was also able to agglutinate <em>V. splendidus</em>, and inhibit <em>V. splendidus</em> growth. Furthermore, r<em>Cg</em>Gal-CUB exhibited the activities of enhancing the haemocyte phagocytosis towards <em>V. splendidus</em>, and the phagocytosis rate of haemocytes was descended in blockage assay with <em>C</em>gGal-CUB antibody. These results suggested that <em>Cg</em>Gal-CUB served as a pattern recognition receptor to bind various PAMPs and bacteria, and enhanced the haemocyte phagocytosis towards <em>V. splendidus</em>.</p></div>\",\"PeriodicalId\":11228,\"journal\":{\"name\":\"Developmental and comparative immunology\",\"volume\":\"159 \",\"pages\":\"Article 105222\"},\"PeriodicalIF\":2.7000,\"publicationDate\":\"2024-07-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Developmental and comparative immunology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0145305X24000946\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"FISHERIES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Developmental and comparative immunology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0145305X24000946","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"FISHERIES","Score":null,"Total":0}
A novel lectin with a distinct Gal_Lectin and CUB domain mediates haemocyte phagocytosis in oyster Crassostrea gigas
Invertebrate lectins exhibit structural diversity and play crucial roles in the innate immune responses by recognizing and eliminating pathogens. In the present study, a novel lectin containing a Gal_Lectin, a CUB and a transmembrane domain was identified from the Pacific oyster Crassostrea gigas (defined as CgGal-CUB). CgGal-CUB mRNA was detectable in all the examined tissues with the highest expression in adductor muscle (11.00-fold of that in haemocytes, p < 0.05). The expression level of CgGal-CUB mRNA in haemocytes was significantly up-regulated at 3, 24, 48 and 72 h (8.37-fold, 12.13-fold, 4.28-fold and 10.14-fold of that in the control group, respectively) after Vibrio splendidus stimulation. The recombinant CgGal-CUB (rCgGal-CUB) displayed binding capability to Mannan (MAN), peptidoglycan (PGN), D-(+)-Galactose and L-Rhamnose monohydrate, as well as Gram-negative bacteria (Escherichia coli, V. splendidus and Vibrio anguillarum), Gram-positive bacteria (Micrococcus luteus, Staphylococcus aureus, and Bacillus sybtilis) and fungus (Pichia pastoris). rCgGal-CUB was also able to agglutinate V. splendidus, and inhibit V. splendidus growth. Furthermore, rCgGal-CUB exhibited the activities of enhancing the haemocyte phagocytosis towards V. splendidus, and the phagocytosis rate of haemocytes was descended in blockage assay with CgGal-CUB antibody. These results suggested that CgGal-CUB served as a pattern recognition receptor to bind various PAMPs and bacteria, and enhanced the haemocyte phagocytosis towards V. splendidus.
期刊介绍:
Developmental and Comparative Immunology (DCI) is an international journal that publishes articles describing original research in all areas of immunology, including comparative aspects of immunity and the evolution and development of the immune system. Manuscripts describing studies of immune systems in both vertebrates and invertebrates are welcome. All levels of immunological investigations are appropriate: organismal, cellular, biochemical and molecular genetics, extending to such fields as aging of the immune system, interaction between the immune and neuroendocrine system and intestinal immunity.