{"title":"离体芽增殖和茉莉酸甲酯对增殖芽中黄酮类化合物的诱导作用","authors":"Mahboubeh Abedi, Farah Karimi, Azra Saboora","doi":"10.1007/s11240-024-02804-7","DOIUrl":null,"url":null,"abstract":"<p><i>Haplophyllum virgatum</i> var. <i>virgatum</i> is an Iranian endemic plant. Its only natural habitat is the Geno region in the Hormozgan province of Iran. This plant’s limited distribution and special habitat conditions prompted us to investigate the possibility of its micropropagation and different biological aspects. In this study, while evaluating seed germination and seedling production of <i>H. virgatum</i> in two Murashige and Skoog (MS) and Gamborg (B5) culture media, shoot multiplication and rooting of single-node shoot explants of this plant were also investigated in the MS culture medium containing different concentrations of growth regulators. Flavonoids are part of the medicinal metabolites of different <i>Haplophyllum</i> species. Here, the flavonoid elicitation in the proliferated shoots of <i>H. virgatum</i> under 0, 100, and 150 µM methyl jasmonate (MeJA) treatments was done. The results demonstrated that the MS culture medium was more suitable than B5 for seed germination and natural growth of seedlings. Moreover, for <i>H. virgatum</i> shoot multiplication, one mg/L kinetin was more appropriate compared to other concentrations of this cytokinin (0.5, 1.5, 2, 2.5, and 3 mg/L) and the corresponding concentrations of benzyl amino purine. Rooting was achieved only in a limited number of shoots, so it needs further investigation. Meanwhile, MeJA increased total phenolic, total flavonoid, and rutin (the main flavonoid of Rutaceae) contents in the proliferated shoots. This research provided new information about the micropropagation of <i>H. virgatum</i> and flavonoid elicitation by MeJA in this plant.</p>","PeriodicalId":20219,"journal":{"name":"Plant Cell, Tissue and Organ Culture","volume":"1 1","pages":""},"PeriodicalIF":2.3000,"publicationDate":"2024-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"In vitro shoot multiplication of Haplophyllum virgatum and flavonoid elicitation in proliferated shoots by methyl jasmonate\",\"authors\":\"Mahboubeh Abedi, Farah Karimi, Azra Saboora\",\"doi\":\"10.1007/s11240-024-02804-7\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><i>Haplophyllum virgatum</i> var. <i>virgatum</i> is an Iranian endemic plant. Its only natural habitat is the Geno region in the Hormozgan province of Iran. This plant’s limited distribution and special habitat conditions prompted us to investigate the possibility of its micropropagation and different biological aspects. In this study, while evaluating seed germination and seedling production of <i>H. virgatum</i> in two Murashige and Skoog (MS) and Gamborg (B5) culture media, shoot multiplication and rooting of single-node shoot explants of this plant were also investigated in the MS culture medium containing different concentrations of growth regulators. Flavonoids are part of the medicinal metabolites of different <i>Haplophyllum</i> species. Here, the flavonoid elicitation in the proliferated shoots of <i>H. virgatum</i> under 0, 100, and 150 µM methyl jasmonate (MeJA) treatments was done. The results demonstrated that the MS culture medium was more suitable than B5 for seed germination and natural growth of seedlings. Moreover, for <i>H. virgatum</i> shoot multiplication, one mg/L kinetin was more appropriate compared to other concentrations of this cytokinin (0.5, 1.5, 2, 2.5, and 3 mg/L) and the corresponding concentrations of benzyl amino purine. Rooting was achieved only in a limited number of shoots, so it needs further investigation. Meanwhile, MeJA increased total phenolic, total flavonoid, and rutin (the main flavonoid of Rutaceae) contents in the proliferated shoots. 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引用次数: 0
摘要
Haplophyllum virgatum var. virgatum 是一种伊朗特有植物。它唯一的自然栖息地是伊朗霍尔木兹甘省的基诺地区。这种植物的有限分布和特殊的栖息地条件促使我们对其微繁殖的可能性和不同的生物学方面进行研究。在本研究中,我们在两种 Murashige and Skoog(MS)和 Gamborg(B5)培养基中评估了 H. virgatum 的种子萌发和幼苗培育情况,同时还在含有不同浓度生长调节剂的 MS 培养基中研究了该植物单节芽外植体的芽繁殖和生根情况。黄酮类化合物是不同合欢属植物药用代谢产物的一部分。在此,研究人员分别在 0、100 和 150 µM 的茉莉酸甲酯(MeJA)处理条件下,研究了黄酮类化合物在虎尾兰增殖嫩枝中的激发情况。结果表明,MS 培养基比 B5 培养基更适合种子萌发和幼苗的自然生长。此外,与其他浓度的细胞分裂素(0.5、1.5、2、2.5 和 3 毫克/升)和相应浓度的苄基氨基嘌呤相比,1 毫克/升的细胞分裂素更适合病毒菜的芽繁殖。只有少数嫩芽实现了生根,因此还需要进一步研究。同时,MeJA 增加了增殖芽中总酚、总黄酮和芦丁(芦竹科主要黄酮类化合物)的含量。该研究为 virgatum 的微繁殖和 MeJA 对该植物黄酮类化合物的激发提供了新的信息。
In vitro shoot multiplication of Haplophyllum virgatum and flavonoid elicitation in proliferated shoots by methyl jasmonate
Haplophyllum virgatum var. virgatum is an Iranian endemic plant. Its only natural habitat is the Geno region in the Hormozgan province of Iran. This plant’s limited distribution and special habitat conditions prompted us to investigate the possibility of its micropropagation and different biological aspects. In this study, while evaluating seed germination and seedling production of H. virgatum in two Murashige and Skoog (MS) and Gamborg (B5) culture media, shoot multiplication and rooting of single-node shoot explants of this plant were also investigated in the MS culture medium containing different concentrations of growth regulators. Flavonoids are part of the medicinal metabolites of different Haplophyllum species. Here, the flavonoid elicitation in the proliferated shoots of H. virgatum under 0, 100, and 150 µM methyl jasmonate (MeJA) treatments was done. The results demonstrated that the MS culture medium was more suitable than B5 for seed germination and natural growth of seedlings. Moreover, for H. virgatum shoot multiplication, one mg/L kinetin was more appropriate compared to other concentrations of this cytokinin (0.5, 1.5, 2, 2.5, and 3 mg/L) and the corresponding concentrations of benzyl amino purine. Rooting was achieved only in a limited number of shoots, so it needs further investigation. Meanwhile, MeJA increased total phenolic, total flavonoid, and rutin (the main flavonoid of Rutaceae) contents in the proliferated shoots. This research provided new information about the micropropagation of H. virgatum and flavonoid elicitation by MeJA in this plant.
期刊介绍:
This journal highlights the myriad breakthrough technologies and discoveries in plant biology and biotechnology. Plant Cell, Tissue and Organ Culture (PCTOC: Journal of Plant Biotechnology) details high-throughput analysis of gene function and expression, gene silencing and overexpression analyses, RNAi, siRNA, and miRNA studies, and much more. It examines the transcriptional and/or translational events involved in gene regulation as well as those molecular controls involved in morphogenesis of plant cells and tissues.
The journal also covers practical and applied plant biotechnology, including regeneration, organogenesis and somatic embryogenesis, gene transfer, gene flow, secondary metabolites, metabolic engineering, and impact of transgene(s) dissemination into managed and unmanaged plant systems.