蔷薇藻胼胝体培养物中高价值代谢物的定性和定量分析

IF 2.3 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Hasnat Tariq, Anisa Andleeb, Sannia Batool, Urooj Ali, Muhammad Waqas, Abdul Wahab, Gouhar Zaman, Samantha Drouet, Nathalie Giglioli-Guivarc’h, Christophe Hano, Bilal Haider Abbasi
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引用次数: 0

摘要

本研究探讨了植物生长调节剂(PGRs)如何影响Alcea rosea L.胼胝体培养物的胼胝体诱导、生物量积累、植物化学物质合成以及抗氧化和抗衰老潜力。我们的研究结果表明,1 µM NAA 和 0.1 µM TDZ 的组合可导致 100% 的胼胝体诱导率和最高的生物量积累,鲜重为 267 克/升,干重为 16.5 克/升。在用 1 µM NAA 处理的胼胝体培养物中,观察到总酚(719.19 mg GAE/L)和总黄酮(166.34 mg QE/L)产量最高。高效液相色谱法鉴定出 9 种花青素化合物,其中 PGR 浓度为 1 µM NAA + 0.1 µM TDZ 时花青素总产量最高(265.65 µM DW)。不同 PGR 浓度的抗氧化活性各不相同,显著值包括 DPPH-FRSA 为 83.1%,ORAC 值为 1089.13 µM TEAC,ROS/RNS 水平最大降低 87.56%。抗衰老活性也各不相同,对酪氨酸酶的抑制率最高为 79.12%,对类 vesperlysine AGEs 的抑制率为 68.32%,对类 pentosidine AGEs 的抑制率为 56.42%。这项研究引入了一种新方法,通过研究 PGRs 对胼胝体培养启动和次生代谢物生产的影响以及抗氧化和抗衰老特性,解决了传统提取方法的局限性。这种方法有可能增加我们对蔷薇科植物治疗潜力的了解,并有助于开发生产生物活性化合物的可持续技术。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Qualitative and quantitative analysis of high-value metabolites from callus culture of Alcea rosea L.

Qualitative and quantitative analysis of high-value metabolites from callus culture of Alcea rosea L.

This study explored how plant growth regulators (PGRs) impact callus induction, biomass accumulation, phytochemical synthesis, and the antioxidant and anti-aging potential of Alcea rosea L. callus cultures. Our findings revealed that the combination of 1 µM NAA and 0.1 µM TDZ leads to 100% callus induction frequency and the highest biomass accumulation, with a fresh weight of 267 g/L and a dry weight of 16.5 g/L. Maximum total phenolic (719.19 mg GAE/L) and total flavonoid (166.34 mg QE/L) production were observed in callus cultures treated with 1 µM NAA. High-performance liquid chromatography identified 9 anthocyanin compounds, with the highest total anthocyanin production (265.65 µM DW) recorded with a PGR concentration of 1 µM NAA + 0.1 µM TDZ. Antioxidant activities varied across different PGR concentrations, with notable values including DPPH-FRSA of 83.1%, ORAC value of 1089.13 µM TEAC, and maximum reduction in ROS/RNS levels by 87.56%. Anti-aging activities also varied, with tyrosinase inhibition peaking at 79.12%, vesperlysine-like AGEs’ inhibition at 68.32%, and pentosidine-like AGEs’ inhibition at 56.42%. This study introduces a novel approach that addresses traditional extraction method limitations by examining the impact of PGRs on callus culture initiation and secondary metabolite production, alongside antioxidative and anti-aging characteristics. Such methodologies potentially add to our understanding of A. rosea L.‘s therapeutic potential and contribute to sustainable techniques for producing bioactive compounds.

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来源期刊
Plant Cell, Tissue and Organ Culture
Plant Cell, Tissue and Organ Culture 生物-生物工程与应用微生物
CiteScore
5.40
自引率
13.30%
发文量
203
审稿时长
3.3 months
期刊介绍: This journal highlights the myriad breakthrough technologies and discoveries in plant biology and biotechnology. Plant Cell, Tissue and Organ Culture (PCTOC: Journal of Plant Biotechnology) details high-throughput analysis of gene function and expression, gene silencing and overexpression analyses, RNAi, siRNA, and miRNA studies, and much more. It examines the transcriptional and/or translational events involved in gene regulation as well as those molecular controls involved in morphogenesis of plant cells and tissues. The journal also covers practical and applied plant biotechnology, including regeneration, organogenesis and somatic embryogenesis, gene transfer, gene flow, secondary metabolites, metabolic engineering, and impact of transgene(s) dissemination into managed and unmanaged plant systems.
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