Phan Le Ha Nguyen, Nguyen Thi Thanh Thuy, Nguyen Thi Nhu Mai, Ho Cam Khanh Hoa, Hoang Dac Khai, Hoang Thanh Tung, Do Manh Cuong, Vu Quoc Luan, Hoang Thi Nhu Phuong, Nguyen Quang Vinh, Doan Manh Dung, Duong Tan Nhut
{"title":"MoO3NPs对离体培养菊花的再生、生长和发育的作用","authors":"Phan Le Ha Nguyen, Nguyen Thi Thanh Thuy, Nguyen Thi Nhu Mai, Ho Cam Khanh Hoa, Hoang Dac Khai, Hoang Thanh Tung, Do Manh Cuong, Vu Quoc Luan, Hoang Thi Nhu Phuong, Nguyen Quang Vinh, Doan Manh Dung, Duong Tan Nhut","doi":"10.1007/s11240-024-02806-5","DOIUrl":null,"url":null,"abstract":"<p>This study substituted sodium molybdate dehydrate (Na<sub>2</sub>MoO<sub>4</sub>.2H<sub>2</sub>O) in MS medium (Murashige and Skoog 1962) with molybdenum trioxide nanoparticles (MoO<sub>3</sub>NPs) to evaluate their impact on the morphogenesis, growth, absorption of metal-mineral elements and the activity of antioxidant enzymes of chrysanthemum. The results indicated that 100% callus formation was observed when 1-month-old leaf explants were cultured on medium supplemented with MoO<sub>3</sub>NPs and Na<sub>2</sub>MoO<sub>4</sub>.2H<sub>2</sub>O in basic MS medium, while leaf explants cultured on MS medium without Na<sub>2</sub>MoO<sub>4</sub>.2H<sub>2</sub>O did not induce callus induction. In addition, the treatment with 223.5 µL/L MoO<sub>3</sub>NPs resulted in the highest shoot regeneration (33.33%), with 1 shoot per explant, and shoot height (1.14 cm), and fresh weight (1.21 g) compared to those in others and control treatments. Meanwhile, 1-month-old stem node (1 cm) explants cultured on medium supplemented with 149 µL/L MoO<sub>3</sub>NPs recorded 100% shoot regeneration and the highest number of shoots larger than 2 cm (5 shoots/stem node), shoot height (3.23 cm) and fresh weight (1.87 g) of the shoot cluster after 30 days of culture. During the regeneration stage, the activity of antioxidant enzymes in 149 µg/L MoO<sub>3</sub>NPs treatment was better than the control (+) treatment (except for SOD) and all the other treatments. Similar results were also observed during the shoot multiplication phase, where either the lack or surplus of Mo in the culture medium also caused the decline of SOD, CAT, and APX enzymes activity. Besides, at the concentration of 6.4 µg/L MoO<sub>3</sub>NPs in the culture media, nutrients are absorbed more efficiently and rapidly by explants. These findings suggest that substituting ion salt in the culture medium with MoO<sub>3</sub>NPs led to enhanced absorption, providing a micro-mineral source for plants to support biosynthesis and essential functions. The chrysanthemum plantlets exhibited enhanced rooting and growth when treated with 149 µg/L MoO<sub>3</sub>NPs, particularly during the rooting stage after 15 days of culture.</p>","PeriodicalId":20219,"journal":{"name":"Plant Cell, Tissue and Organ Culture","volume":"15 1","pages":""},"PeriodicalIF":2.3000,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The role of MoO3NPs on regeneration, growth and development of chrysanthemum cultured in vitro\",\"authors\":\"Phan Le Ha Nguyen, Nguyen Thi Thanh Thuy, Nguyen Thi Nhu Mai, Ho Cam Khanh Hoa, Hoang Dac Khai, Hoang Thanh Tung, Do Manh Cuong, Vu Quoc Luan, Hoang Thi Nhu Phuong, Nguyen Quang Vinh, Doan Manh Dung, Duong Tan Nhut\",\"doi\":\"10.1007/s11240-024-02806-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>This study substituted sodium molybdate dehydrate (Na<sub>2</sub>MoO<sub>4</sub>.2H<sub>2</sub>O) in MS medium (Murashige and Skoog 1962) with molybdenum trioxide nanoparticles (MoO<sub>3</sub>NPs) to evaluate their impact on the morphogenesis, growth, absorption of metal-mineral elements and the activity of antioxidant enzymes of chrysanthemum. The results indicated that 100% callus formation was observed when 1-month-old leaf explants were cultured on medium supplemented with MoO<sub>3</sub>NPs and Na<sub>2</sub>MoO<sub>4</sub>.2H<sub>2</sub>O in basic MS medium, while leaf explants cultured on MS medium without Na<sub>2</sub>MoO<sub>4</sub>.2H<sub>2</sub>O did not induce callus induction. In addition, the treatment with 223.5 µL/L MoO<sub>3</sub>NPs resulted in the highest shoot regeneration (33.33%), with 1 shoot per explant, and shoot height (1.14 cm), and fresh weight (1.21 g) compared to those in others and control treatments. Meanwhile, 1-month-old stem node (1 cm) explants cultured on medium supplemented with 149 µL/L MoO<sub>3</sub>NPs recorded 100% shoot regeneration and the highest number of shoots larger than 2 cm (5 shoots/stem node), shoot height (3.23 cm) and fresh weight (1.87 g) of the shoot cluster after 30 days of culture. During the regeneration stage, the activity of antioxidant enzymes in 149 µg/L MoO<sub>3</sub>NPs treatment was better than the control (+) treatment (except for SOD) and all the other treatments. Similar results were also observed during the shoot multiplication phase, where either the lack or surplus of Mo in the culture medium also caused the decline of SOD, CAT, and APX enzymes activity. Besides, at the concentration of 6.4 µg/L MoO<sub>3</sub>NPs in the culture media, nutrients are absorbed more efficiently and rapidly by explants. These findings suggest that substituting ion salt in the culture medium with MoO<sub>3</sub>NPs led to enhanced absorption, providing a micro-mineral source for plants to support biosynthesis and essential functions. The chrysanthemum plantlets exhibited enhanced rooting and growth when treated with 149 µg/L MoO<sub>3</sub>NPs, particularly during the rooting stage after 15 days of culture.</p>\",\"PeriodicalId\":20219,\"journal\":{\"name\":\"Plant Cell, Tissue and Organ Culture\",\"volume\":\"15 1\",\"pages\":\"\"},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2024-06-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant Cell, Tissue and Organ Culture\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s11240-024-02806-5\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Cell, Tissue and Organ Culture","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s11240-024-02806-5","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
The role of MoO3NPs on regeneration, growth and development of chrysanthemum cultured in vitro
This study substituted sodium molybdate dehydrate (Na2MoO4.2H2O) in MS medium (Murashige and Skoog 1962) with molybdenum trioxide nanoparticles (MoO3NPs) to evaluate their impact on the morphogenesis, growth, absorption of metal-mineral elements and the activity of antioxidant enzymes of chrysanthemum. The results indicated that 100% callus formation was observed when 1-month-old leaf explants were cultured on medium supplemented with MoO3NPs and Na2MoO4.2H2O in basic MS medium, while leaf explants cultured on MS medium without Na2MoO4.2H2O did not induce callus induction. In addition, the treatment with 223.5 µL/L MoO3NPs resulted in the highest shoot regeneration (33.33%), with 1 shoot per explant, and shoot height (1.14 cm), and fresh weight (1.21 g) compared to those in others and control treatments. Meanwhile, 1-month-old stem node (1 cm) explants cultured on medium supplemented with 149 µL/L MoO3NPs recorded 100% shoot regeneration and the highest number of shoots larger than 2 cm (5 shoots/stem node), shoot height (3.23 cm) and fresh weight (1.87 g) of the shoot cluster after 30 days of culture. During the regeneration stage, the activity of antioxidant enzymes in 149 µg/L MoO3NPs treatment was better than the control (+) treatment (except for SOD) and all the other treatments. Similar results were also observed during the shoot multiplication phase, where either the lack or surplus of Mo in the culture medium also caused the decline of SOD, CAT, and APX enzymes activity. Besides, at the concentration of 6.4 µg/L MoO3NPs in the culture media, nutrients are absorbed more efficiently and rapidly by explants. These findings suggest that substituting ion salt in the culture medium with MoO3NPs led to enhanced absorption, providing a micro-mineral source for plants to support biosynthesis and essential functions. The chrysanthemum plantlets exhibited enhanced rooting and growth when treated with 149 µg/L MoO3NPs, particularly during the rooting stage after 15 days of culture.
期刊介绍:
This journal highlights the myriad breakthrough technologies and discoveries in plant biology and biotechnology. Plant Cell, Tissue and Organ Culture (PCTOC: Journal of Plant Biotechnology) details high-throughput analysis of gene function and expression, gene silencing and overexpression analyses, RNAi, siRNA, and miRNA studies, and much more. It examines the transcriptional and/or translational events involved in gene regulation as well as those molecular controls involved in morphogenesis of plant cells and tissues.
The journal also covers practical and applied plant biotechnology, including regeneration, organogenesis and somatic embryogenesis, gene transfer, gene flow, secondary metabolites, metabolic engineering, and impact of transgene(s) dissemination into managed and unmanaged plant systems.