{"title":"基于碳量子点的无标记荧光生物传感器检测大肠杆菌","authors":"Xiaolian Bai;Lu Ga;Yanqing Du;Jun Ai","doi":"10.1109/JSEN.2024.3419135","DOIUrl":null,"url":null,"abstract":"We prepared carbon quantum dots (CQDs) by hydrothermal method using celery juice as carbon source. And the fluorescence of CQDs was burst by silver nanoparticles (Ag NPs) through inner filter effect (IFE), using Ag NPs as absorbers to construct label-free sensor. This strategy relies on the absorption spectra of Ag NPs overlapping with the fluorescence excitation or emission of fluorophores and the specific binding ability of \n<inline-formula> <tex-math>$\\textit {Escherichia coli (E. coli)}$ </tex-math></inline-formula>\n aptamers with target. The free aptamer sequence is first depleted to form complexes, leading to Ag NPs aggregation at high salt concentrations when adding E. coli to system. At this point, the absorption spectrum of Ag NPs changes and no longer overlaps with the fluorescence emission spectrum of CQDs, leading to a significant fluorescence recovery of the system. The excitation wavelength of the prepared CQDs was 348 nm and the maximum emission wavelength was 514 nm. The average particle size was about 8.84 nm with uniform dispersion. The linear range of this sensor was \n<inline-formula> <tex-math>$2\\times 10^{\\mathbf {{2}}}\\sim ~2\\times 10^{\\mathbf {{7}}}$ </tex-math></inline-formula>\nCFU\n<inline-formula> <tex-math>$\\cdot $ </tex-math></inline-formula>\nmL\n<inline-formula> <tex-math>$^{\\mathbf {-{1}}}$ </tex-math></inline-formula>\n, and the detection limit for E. coli was as low as 185 CFU\n<inline-formula> <tex-math>$\\cdot $ </tex-math></inline-formula>\nmL\n<inline-formula> <tex-math>$^{\\mathbf {-{1}}}$ </tex-math></inline-formula>\n.","PeriodicalId":447,"journal":{"name":"IEEE Sensors Journal","volume":null,"pages":null},"PeriodicalIF":4.3000,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Carbon Quantum Dot-Based Label-Free Fluorescent Biosensor to Detect E. coli\",\"authors\":\"Xiaolian Bai;Lu Ga;Yanqing Du;Jun Ai\",\"doi\":\"10.1109/JSEN.2024.3419135\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"We prepared carbon quantum dots (CQDs) by hydrothermal method using celery juice as carbon source. And the fluorescence of CQDs was burst by silver nanoparticles (Ag NPs) through inner filter effect (IFE), using Ag NPs as absorbers to construct label-free sensor. This strategy relies on the absorption spectra of Ag NPs overlapping with the fluorescence excitation or emission of fluorophores and the specific binding ability of \\n<inline-formula> <tex-math>$\\\\textit {Escherichia coli (E. coli)}$ </tex-math></inline-formula>\\n aptamers with target. The free aptamer sequence is first depleted to form complexes, leading to Ag NPs aggregation at high salt concentrations when adding E. coli to system. At this point, the absorption spectrum of Ag NPs changes and no longer overlaps with the fluorescence emission spectrum of CQDs, leading to a significant fluorescence recovery of the system. The excitation wavelength of the prepared CQDs was 348 nm and the maximum emission wavelength was 514 nm. The average particle size was about 8.84 nm with uniform dispersion. The linear range of this sensor was \\n<inline-formula> <tex-math>$2\\\\times 10^{\\\\mathbf {{2}}}\\\\sim ~2\\\\times 10^{\\\\mathbf {{7}}}$ </tex-math></inline-formula>\\nCFU\\n<inline-formula> <tex-math>$\\\\cdot $ </tex-math></inline-formula>\\nmL\\n<inline-formula> <tex-math>$^{\\\\mathbf {-{1}}}$ </tex-math></inline-formula>\\n, and the detection limit for E. coli was as low as 185 CFU\\n<inline-formula> <tex-math>$\\\\cdot $ </tex-math></inline-formula>\\nmL\\n<inline-formula> <tex-math>$^{\\\\mathbf {-{1}}}$ </tex-math></inline-formula>\\n.\",\"PeriodicalId\":447,\"journal\":{\"name\":\"IEEE Sensors Journal\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.3000,\"publicationDate\":\"2024-07-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"IEEE Sensors Journal\",\"FirstCategoryId\":\"103\",\"ListUrlMain\":\"https://ieeexplore.ieee.org/document/10582819/\",\"RegionNum\":2,\"RegionCategory\":\"综合性期刊\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENGINEERING, ELECTRICAL & ELECTRONIC\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"IEEE Sensors Journal","FirstCategoryId":"103","ListUrlMain":"https://ieeexplore.ieee.org/document/10582819/","RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, ELECTRICAL & ELECTRONIC","Score":null,"Total":0}
Carbon Quantum Dot-Based Label-Free Fluorescent Biosensor to Detect E. coli
We prepared carbon quantum dots (CQDs) by hydrothermal method using celery juice as carbon source. And the fluorescence of CQDs was burst by silver nanoparticles (Ag NPs) through inner filter effect (IFE), using Ag NPs as absorbers to construct label-free sensor. This strategy relies on the absorption spectra of Ag NPs overlapping with the fluorescence excitation or emission of fluorophores and the specific binding ability of
$\textit {Escherichia coli (E. coli)}$
aptamers with target. The free aptamer sequence is first depleted to form complexes, leading to Ag NPs aggregation at high salt concentrations when adding E. coli to system. At this point, the absorption spectrum of Ag NPs changes and no longer overlaps with the fluorescence emission spectrum of CQDs, leading to a significant fluorescence recovery of the system. The excitation wavelength of the prepared CQDs was 348 nm and the maximum emission wavelength was 514 nm. The average particle size was about 8.84 nm with uniform dispersion. The linear range of this sensor was
$2\times 10^{\mathbf {{2}}}\sim ~2\times 10^{\mathbf {{7}}}$
CFU
$\cdot $
mL
$^{\mathbf {-{1}}}$
, and the detection limit for E. coli was as low as 185 CFU
$\cdot $
mL
$^{\mathbf {-{1}}}$
.
期刊介绍:
The fields of interest of the IEEE Sensors Journal are the theory, design , fabrication, manufacturing and applications of devices for sensing and transducing physical, chemical and biological phenomena, with emphasis on the electronics and physics aspect of sensors and integrated sensors-actuators. IEEE Sensors Journal deals with the following:
-Sensor Phenomenology, Modelling, and Evaluation
-Sensor Materials, Processing, and Fabrication
-Chemical and Gas Sensors
-Microfluidics and Biosensors
-Optical Sensors
-Physical Sensors: Temperature, Mechanical, Magnetic, and others
-Acoustic and Ultrasonic Sensors
-Sensor Packaging
-Sensor Networks
-Sensor Applications
-Sensor Systems: Signals, Processing, and Interfaces
-Actuators and Sensor Power Systems
-Sensor Signal Processing for high precision and stability (amplification, filtering, linearization, modulation/demodulation) and under harsh conditions (EMC, radiation, humidity, temperature); energy consumption/harvesting
-Sensor Data Processing (soft computing with sensor data, e.g., pattern recognition, machine learning, evolutionary computation; sensor data fusion, processing of wave e.g., electromagnetic and acoustic; and non-wave, e.g., chemical, gravity, particle, thermal, radiative and non-radiative sensor data, detection, estimation and classification based on sensor data)
-Sensors in Industrial Practice