Wanlu Zhu, Rui Xiao, Min Xu, Wenbo Chai, Wenlong Liu, Zhengyu Jin, David Ikumi, Huijie Lu
{"title":"揭示甲酸盐在通过部分反硝化-氨氧化作用耦合提高脱氮能力中的作用","authors":"Wanlu Zhu, Rui Xiao, Min Xu, Wenbo Chai, Wenlong Liu, Zhengyu Jin, David Ikumi, Huijie Lu","doi":"10.1007/s11783-024-1872-8","DOIUrl":null,"url":null,"abstract":"<p>The addition of traditional carbon sources (e.g., acetate) could favor heterotrophic overgrowth in partial denitrification coupled with anammox (PD–A) systems, thus hindering the performance and stability of this novel wastewater nitrogen removal technology. Therefore, it is necessary to develop an effective, environmentally friendly, and inexpensive alternative. This study demonstrated the potential of formate to enhance the performance and community stability of PD–A under mainstream conditions. In a laboratory-scale biofilm reactor, formate addition (COD/NO<sub>3</sub><sup>−</sup>–N = 1.75) improved nitrogen removal efficiency (from 72.1 ± 3.5% to 81.7 ± 2.7%), EPS content (from 106.3 ± 8.1 to 163.0 ± 15.5 mg/gVSS) and increased anammox bacteria growth (predominantly <i>Candidatus</i> Brocadia, from 29.5 ± 0.7% to 34.5 ± 5.4%) while maintaining stable heterotrophs dominated by methylotrophic <i>Desulfobacillus</i>. FISH-NanoSIMS revealed a formate uptake using <i>Ca.</i> Brocadia and <i>Desulfobacillus</i>, with <i>Ca.</i> Brocadia being the major contributor to partial nitrate reduction to nitrite. <i>Desulfobacillus</i> can synthesize diverse hydrophobic amino acids and provide key nutrients for <i>Ca.</i> Brocadia. To achieve comparable nitrogen removal, the cost of the formate-driven PD–A process should be 11.2% lower than that of acetate. These results greatly enrich our understanding of C1 metabolism represented by formate in anammox communities and its application in the context of coupling partial denitrification-anammox toward enhanced nitrogen removal in global wastewater treatment systems.\n</p>","PeriodicalId":12720,"journal":{"name":"Frontiers of Environmental Science & Engineering","volume":null,"pages":null},"PeriodicalIF":6.1000,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Unraveling the role of formate in improving nitrogen removal via coupled partial denitrification-anammox\",\"authors\":\"Wanlu Zhu, Rui Xiao, Min Xu, Wenbo Chai, Wenlong Liu, Zhengyu Jin, David Ikumi, Huijie Lu\",\"doi\":\"10.1007/s11783-024-1872-8\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>The addition of traditional carbon sources (e.g., acetate) could favor heterotrophic overgrowth in partial denitrification coupled with anammox (PD–A) systems, thus hindering the performance and stability of this novel wastewater nitrogen removal technology. Therefore, it is necessary to develop an effective, environmentally friendly, and inexpensive alternative. This study demonstrated the potential of formate to enhance the performance and community stability of PD–A under mainstream conditions. In a laboratory-scale biofilm reactor, formate addition (COD/NO<sub>3</sub><sup>−</sup>–N = 1.75) improved nitrogen removal efficiency (from 72.1 ± 3.5% to 81.7 ± 2.7%), EPS content (from 106.3 ± 8.1 to 163.0 ± 15.5 mg/gVSS) and increased anammox bacteria growth (predominantly <i>Candidatus</i> Brocadia, from 29.5 ± 0.7% to 34.5 ± 5.4%) while maintaining stable heterotrophs dominated by methylotrophic <i>Desulfobacillus</i>. FISH-NanoSIMS revealed a formate uptake using <i>Ca.</i> Brocadia and <i>Desulfobacillus</i>, with <i>Ca.</i> Brocadia being the major contributor to partial nitrate reduction to nitrite. <i>Desulfobacillus</i> can synthesize diverse hydrophobic amino acids and provide key nutrients for <i>Ca.</i> Brocadia. To achieve comparable nitrogen removal, the cost of the formate-driven PD–A process should be 11.2% lower than that of acetate. 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Unraveling the role of formate in improving nitrogen removal via coupled partial denitrification-anammox
The addition of traditional carbon sources (e.g., acetate) could favor heterotrophic overgrowth in partial denitrification coupled with anammox (PD–A) systems, thus hindering the performance and stability of this novel wastewater nitrogen removal technology. Therefore, it is necessary to develop an effective, environmentally friendly, and inexpensive alternative. This study demonstrated the potential of formate to enhance the performance and community stability of PD–A under mainstream conditions. In a laboratory-scale biofilm reactor, formate addition (COD/NO3−–N = 1.75) improved nitrogen removal efficiency (from 72.1 ± 3.5% to 81.7 ± 2.7%), EPS content (from 106.3 ± 8.1 to 163.0 ± 15.5 mg/gVSS) and increased anammox bacteria growth (predominantly Candidatus Brocadia, from 29.5 ± 0.7% to 34.5 ± 5.4%) while maintaining stable heterotrophs dominated by methylotrophic Desulfobacillus. FISH-NanoSIMS revealed a formate uptake using Ca. Brocadia and Desulfobacillus, with Ca. Brocadia being the major contributor to partial nitrate reduction to nitrite. Desulfobacillus can synthesize diverse hydrophobic amino acids and provide key nutrients for Ca. Brocadia. To achieve comparable nitrogen removal, the cost of the formate-driven PD–A process should be 11.2% lower than that of acetate. These results greatly enrich our understanding of C1 metabolism represented by formate in anammox communities and its application in the context of coupling partial denitrification-anammox toward enhanced nitrogen removal in global wastewater treatment systems.
期刊介绍:
Frontiers of Environmental Science & Engineering (FESE) is an international journal for researchers interested in a wide range of environmental disciplines. The journal''s aim is to advance and disseminate knowledge in all main branches of environmental science & engineering. The journal emphasizes papers in developing fields, as well as papers showing the interaction between environmental disciplines and other disciplines.
FESE is a bi-monthly journal. Its peer-reviewed contents consist of a broad blend of reviews, research papers, policy analyses, short communications, and opinions. Nonscheduled “special issue” and "hot topic", including a review article followed by a couple of related research articles, are organized to publish novel contributions and breaking results on all aspects of environmental field.