玉米生长根不同区域表达的α-和β-表蛋白

IF 1.1 4区 生物学 Q3 PLANT SCIENCES
T. A. Gorshkova, N. V. Shilova, L. V. Kozlova, O. V. Gorshkov, A. R. Nazipova, A. R. Aglyamova, S. M. Polyakova, A. Yu. Nokel, V. V. Golovchenko, P. V. Mikshina, O. A. Patova, N. V. Bovin
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引用次数: 0

摘要

摘要膨胀蛋白是一种小蛋白,在各种生理过程中,特别是在细胞通过伸长而生长的过程中,在改变细胞壁结构方面起着关键作用。膨胀蛋白由一个庞大的多基因家族编码,分为四个亚家族,主要是α-和β-膨胀蛋白;后者在谷物中得到了特别的发展。扩张素被认为可以改变纤维素与木聚糖(α-扩张素)或阿拉伯木聚糖(β-扩张素)之间的相互作用。同时,扩张素不具有催化活性,其作用的具体机制尚不清楚,如此多种异构体的生理意义也不清楚。为了研究单个扩张素的表达模式,我们利用一个方便的模型系统对玉米基因组中发现的所有扩张素基因进行了转录组分析--玉米主根的生长区在细胞发育阶段和细胞壁组成方面各不相同。在 91 个玉米扩张素基因中,有 67 个在根部表达,大多数扩张素基因的特征是具有最大转录水平的区域范围较窄。通过使用包含来自不同物种植物细胞壁的 183 种多糖的糖谱阵列,我们证明了重组的扩张素 AtEXPA1 和 AtEXPB1 能够与阿拉伯半乳聚糖、鼠李糖半乳聚糖 I 及其他一些细胞壁多糖结合,从而扩大了其潜在的碳水化合物靶标列表。研究表明,α-外显子蛋白和β-外显子蛋白与各种多糖相互作用的特异性在数量和质量上都存在差异。据推测,一种植物机体中扩张素的丰富程度及其表达的精细调节,至少可以部分地通过单个扩张素与特定细胞壁多糖的特异性结合来解释。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Alpha- and Beta-Expansins Expressed in Different Zones of the Growing Root of Maize

Alpha- and Beta-Expansins Expressed in Different Zones of the Growing Root of Maize

Abstract

Expansins are small proteins that play a key role in modifying the structure of the cell wall during various physiological processes, in particular cell growth by elongation. Expansins are encoded by a large multigene family and are divided into four subfamilies, the main ones being alpha- and beta-expansins; the latter have received special development in cereals. Expansins are thought to modify the interactions of cellulose with xyloglucan (alpha-expansins) or with arabinoxylan (beta-expansins). At the same time, expansins do not have catalytic activity, the specific mechanism of their action is unclear, same as the physiological significance of such a wide variety of isoforms. To study the expression patterns of individual expansins, we conducted a transcriptomic analysis of all expansin genes identified in the maize genome using a convenient model system—zones of the maize growing primary root that differ in the stage of cell development and the composition of their cell walls. Of the 91 maize expansin genes, 67 were expressed in the root, and most expansin genes were characterized by a narrow range of zones with maximum transcript levels. Using a glycoarray containing 183 polysaccharides from the cell walls of plants of various species, we demonstrated that recombinant expansins AtEXPA1 and AtEXPB1 are able to bind to arabinogalactans and rhamnogalacturonans I and a number of other cell wall polysaccharides, which expands the list of their potential carbohydrate targets. Differences in the specificity of the interaction of alpha- and beta-expansins with various polysaccharides, both quantitatively and qualitatively, have been demonstrated. It has been hypothesized that the abundance of expansins in one plant organism and the fine regulation of their expression can be explained, at least in part, by the specific binding of individual expansins to specific cell wall polysaccharides.

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来源期刊
CiteScore
4.00
自引率
14.30%
发文量
107
审稿时长
6 months
期刊介绍: Russian Journal of Plant Physiology is a leading journal in phytophysiology. It embraces the full spectrum of plant physiology and brings together the related aspects of biophysics, biochemistry, cytology, anatomy, genetics, etc. The journal publishes experimental and theoretical articles, reviews, short communications, and descriptions of new methods. Some issues cover special problems of plant physiology, thus presenting collections of articles and providing information in rapidly growing fields. The editorial board is highly interested in publishing research from all countries and accepts manuscripts in English.
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