基于掺杂钴-铁-铂-钌/N 的有序介孔碳的 DNA 损伤标记物-8-羟基-2′-脱氧鸟苷的护理点检测技术

IF 2.7 3区 化学 Q2 CHEMISTRY, ANALYTICAL
Yuxin Zhao, Fangxun Liu, Tong Zhang, Man Zheng, Kun Shi, Xin Yang, PinYi Zhao, Xin Li, Yufan Zhang, Pan Li, Huan Wang
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Point-of-care testing of DNA damage markers-8-hydroxy-2′-deoxyguanosine based on cobalt-iron-platinum-ruthenium/N doped ordered mesoporous carbon

In this work, we achieved point of care detection of DNA damage marker 8-hydroxy-2’-deoxyguanosine (8-OH-dG) on tetrapartite metal nanoparticles loaded ordered mesoporous carbon composite nanomaterials (Co-Fex-Pt−Pd@NOMC). Nitrogen doping and co-metal loading were achieved using dopamine as nitrogen, carbon sources and metal linker. In addition, the presence of catechol groups in dopamine with strong affinity for metal ions, make the simultaneous confinement of metals inside and outside the pores of ordered mesoporous carbon. Then, Fe, Pt and Pd metal nanoparticles were introduced to interact with Co nanoparticles to form four-in-one alloy nanoparticles by oil bath and carbonisation. The finally formed Co−Fe6-Pt−Pd@NOMC nanocomposites exhibited excellent catalytic performance for the determination of 8-OH-dG. A lower limit of detection (LOD) of 0.044 μM, a wide linear range (0.175 μM–118.375 μM) and strong stability, reproducibility and selectivity were obtained. In addition, Co−Fe6-Pt−Pd@NOMC realised the detection of 8-OH-dG in real human urine sample. At the same time, 8-OH-dG after DNA damage and guanine damage were also studied in this work. The electrochemical response of 8-OH-dG was combined with DNA damage to verify the mechanism of DNA damage. New solutions and idea were provided for the preparation of inorganic nanocomposites for the detection of 8-OH-dG in this work.

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来源期刊
Electroanalysis
Electroanalysis 化学-电化学
CiteScore
6.00
自引率
3.30%
发文量
222
审稿时长
2.4 months
期刊介绍: Electroanalysis is an international, peer-reviewed journal covering all branches of electroanalytical chemistry, including both fundamental and application papers as well as reviews dealing with new electrochemical sensors and biosensors, nanobioelectronics devices, analytical voltammetry, potentiometry, new electrochemical detection schemes based on novel nanomaterials, fuel cells and biofuel cells, and important practical applications. Serving as a vital communication link between the research labs and the field, Electroanalysis helps you to quickly adapt the latest innovations into practical clinical, environmental, food analysis, industrial and energy-related applications. Electroanalysis provides the most comprehensive coverage of the field and is the number one source for information on electroanalytical chemistry, electrochemical sensors and biosensors and fuel/biofuel cells.
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