{"title":"橙皮甙通过减少 ROS 介导的线粒体损伤和内质网应激，保护 C2C12 肌细胞免受氧化损伤","authors":"Yung Hyun Choi","doi":"10.1007/s13273-024-00446-6","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Background</h3><p>Hesperidin, a flavanone glycoside found primarily in the peel of citrus fruits, has benefits as a natural compound for preventing various diseases, but its inhibitory effect against oxidative injury in muscle cells has not been reported.</p><h3 data-test=\"abstract-sub-heading\">Objective</h3><p>The current study aimed to investigate whether hesperidin can prevent oxidative damage in C2C12 murine myoblasts.</p><h3 data-test=\"abstract-sub-heading\">Results</h3><p>Hesperidin was able to inhibit cytotoxicity while blocking hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) H<sub>2</sub>O<sub>2</sub>-induced DNA damage and apoptosis. Hesperidin also significantly improved the antioxidant capacity of C2C12 cells exposed to H<sub>2</sub>O<sub>2</sub> by suppressing cellular reactive oxygen species production and increasing glutathione level. Additionally, H<sub>2</sub>O<sub>2</sub>-induced mitochondrial dysfunction and endoplasmic reticulum (ER) stress were effectively attenuated in the presence of hesperidin. Moreover, hesperidin neutralized H<sub>2</sub>O<sub>2</sub>-induced calcium ion (Ca<sup>2+</sup>) overload in mitochondria and mitigated the expression of cytosolic Ca<sup>2+</sup>-dependent proteases.</p><h3 data-test=\"abstract-sub-heading\">Conclusion</h3><p>These results imply that hesperidin protects against mitochondrial impairment and Ca<sup>2+</sup>-mediated ER stress by minimizing oxidative stress, thereby suppressing H<sub>2</sub>O<sub>2</sub>-induced cytotoxicity in C2C12 myoblasts.</p>","PeriodicalId":18683,"journal":{"name":"Molecular & Cellular Toxicology","volume":"32 1","pages":""},"PeriodicalIF":1.1000,"publicationDate":"2024-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Hesperidin protects C2C12 myoblasts from oxidative damage by reducing ROS-mediated mitochondrial damage and endoplasmic reticulum stress\",\"authors\":\"Yung Hyun Choi\",\"doi\":\"10.1007/s13273-024-00446-6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<h3 data-test=\\\"abstract-sub-heading\\\">Background</h3><p>Hesperidin, a flavanone glycoside found primarily in the peel of citrus fruits, has benefits as a natural compound for preventing various diseases, but its inhibitory effect against oxidative injury in muscle cells has not been reported.</p><h3 data-test=\\\"abstract-sub-heading\\\">Objective</h3><p>The current study aimed to investigate whether hesperidin can prevent oxidative damage in C2C12 murine myoblasts.</p><h3 data-test=\\\"abstract-sub-heading\\\">Results</h3><p>Hesperidin was able to inhibit cytotoxicity while blocking hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) H<sub>2</sub>O<sub>2</sub>-induced DNA damage and apoptosis. Hesperidin also significantly improved the antioxidant capacity of C2C12 cells exposed to H<sub>2</sub>O<sub>2</sub> by suppressing cellular reactive oxygen species production and increasing glutathione level. Additionally, H<sub>2</sub>O<sub>2</sub>-induced mitochondrial dysfunction and endoplasmic reticulum (ER) stress were effectively attenuated in the presence of hesperidin. Moreover, hesperidin neutralized H<sub>2</sub>O<sub>2</sub>-induced calcium ion (Ca<sup>2+</sup>) overload in mitochondria and mitigated the expression of cytosolic Ca<sup>2+</sup>-dependent proteases.</p><h3 data-test=\\\"abstract-sub-heading\\\">Conclusion</h3><p>These results imply that hesperidin protects against mitochondrial impairment and Ca<sup>2+</sup>-mediated ER stress by minimizing oxidative stress, thereby suppressing H<sub>2</sub>O<sub>2</sub>-induced cytotoxicity in C2C12 myoblasts.</p>\",\"PeriodicalId\":18683,\"journal\":{\"name\":\"Molecular & Cellular Toxicology\",\"volume\":\"32 1\",\"pages\":\"\"},\"PeriodicalIF\":1.1000,\"publicationDate\":\"2024-06-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Molecular & Cellular Toxicology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1007/s13273-024-00446-6\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"TOXICOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular & Cellular Toxicology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s13273-024-00446-6","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"TOXICOLOGY","Score":null,"Total":0}
Hesperidin protects C2C12 myoblasts from oxidative damage by reducing ROS-mediated mitochondrial damage and endoplasmic reticulum stress
Background
Hesperidin, a flavanone glycoside found primarily in the peel of citrus fruits, has benefits as a natural compound for preventing various diseases, but its inhibitory effect against oxidative injury in muscle cells has not been reported.
Objective
The current study aimed to investigate whether hesperidin can prevent oxidative damage in C2C12 murine myoblasts.
Results
Hesperidin was able to inhibit cytotoxicity while blocking hydrogen peroxide (H2O2) H2O2-induced DNA damage and apoptosis. Hesperidin also significantly improved the antioxidant capacity of C2C12 cells exposed to H2O2 by suppressing cellular reactive oxygen species production and increasing glutathione level. Additionally, H2O2-induced mitochondrial dysfunction and endoplasmic reticulum (ER) stress were effectively attenuated in the presence of hesperidin. Moreover, hesperidin neutralized H2O2-induced calcium ion (Ca2+) overload in mitochondria and mitigated the expression of cytosolic Ca2+-dependent proteases.
Conclusion
These results imply that hesperidin protects against mitochondrial impairment and Ca2+-mediated ER stress by minimizing oxidative stress, thereby suppressing H2O2-induced cytotoxicity in C2C12 myoblasts.
期刊介绍：
Molecular & Cellular Toxicology publishes original research and reviews in all areas of the complex interaction between the cell´s genome (the sum of all genes within the chromosome), chemicals in the environment, and disease. Acceptable manuscripts are the ones that deal with some topics of environmental contaminants, including those that lie in the domains of analytical chemistry, biochemistry, pharmacology and toxicology with the aspects of molecular and cellular levels. Emphasis will be placed on toxic effects observed at relevant genomics and proteomics, which have direct impact on drug development, environment health, food safety, preventive medicine, and forensic medicine. The journal is committed to rapid peer review to ensure the publication of highest quality original research and timely news and review articles.
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